Pulse Field Gel Electrophoresis

Sharma-Kuinkel, Batu K.; Rude, Thomas H.; Fowler, Vance G.

doi:10.1007/7651_2014_191

Cited by 38 publications

(30 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…40 The importance of agr locus has been well established in a variety of animal models of infection. 41 Mechanistically, previous studies have demonstrated that the psm-mec RNA influences virulence, at least in part, by inhibiting the translation of agrA , a key positive regulator of all other PSM subtypes. 32,38 Thus, high PSM-mec production should result in lower production of other PSMs, although it has been shown that this effect and the overall impact on virulence of the psm-mec locus is highly strain-dependent.…”

Section: Discussionmentioning

confidence: 99%

Clinical MRSA isolates from skin and soft tissue infections show increased in vitro production of phenol soluble modulins

Berlon

Sharma-Kuinkel

et al. 2015

Journal of Infection

Self Cite

View full text Add to dashboard Cite

Summary Background Phenol-soluble modulins (PSMs) are amphipathic, pro-inflammatory proteins secreted by most Staphylococcus aureus isolates. This study tested the hypothesis that in vitro PSM production levels are associated with specific clinical phenotypes. Methods 177 methicillin-resistant S. aureus (MRSA) isolates from infective endocarditis (IE), skin and soft tissue infection (SSTI), and hospital-acquired/ventilator-associated pneumonia (HAP) were matched by geographic origin, then genotyped using spa-typing. In vitro PSM production was measured by high performance liquid chromatography/mass spectrometry. Statistical analysis was performed using Chi-squared or Kruskal–Wallis tests as appropriate. Results Spa type 1 was significantly more common in SSTI isolates (62.7% SSTI; 1.7% IE; 16.9% HAP; p < 0.0001) while HAP and IE isolates were more commonly spa type 2 (0% SSTI; 37.3% IE; 40.7% HAP; p < 0.0001). USA300 isolates produced the highest levels of PSMs in vitro. SSTI isolates produced significantly higher quantities of PSMα1-4, PSMβ1, and δ-toxin than other isolates (p < 0.001). These findings persisted when USA300 isolates were excluded from analysis.

show abstract

Section: Discussionmentioning

confidence: 99%

Clinical MRSA isolates from skin and soft tissue infections show increased in vitro production of phenol soluble modulins

Berlon

Sharma-Kuinkel

et al. 2015

Journal of Infection

Self Cite

View full text Add to dashboard Cite

show abstract

“…Principle Reference agr typing Amplification of the hypervariable segment [86] spa typing Amplification and sequencing of the 24 bp polymorphic zone X of the spa gene [87] Mutilocus sequence typing (MLST) sequence analysis of the allelic variants of the seven housekeeping genes [88] SCCmec typing Analysis of the structure of SCCmec region [89] Pulsed-field gel electrophoresis (PFGE) Macro-restriction profiling based on the digestion of DNA with restriction enzymes [90] Random Amplification of Polymorphic DNA (RAPD)…”

Section: Typing Methodsmentioning

confidence: 99%

Molecular Epidemiology of Staphylococcus aureus Lineages in Wild Animals in Europe: A Review

et al. 2020

View full text Add to dashboard Cite

Staphylococcus aureus is an opportunist pathogen that is responsible for numerous types of infections. S. aureus is known for its ability to easily acquire antibiotic resistance determinants. Methicillin-resistant S. aureus (MRSA) is a leading cause of infections both in humans and animals and is usually associated with a multidrug-resistant profile. MRSA dissemination is increasing due to its capability of establishing new reservoirs and has been found in humans, animals and the environment. Despite the fact that the information on the incidence of MRSA in the environment and, in particular, in wild animals, is scarce, some studies have reported the presence of these strains among wildlife with no direct contact with antibiotics. This shows a possible transmission between species and, consequently, a public health concern. The aim of this review is to better understand the distribution, prevalence and molecular lineages of MRSA in European free-living animals.

show abstract

“…Organisms involved in acquisition events were analyzed for transmission events using pulsed-field gel electrophoresis (PFGE; see Supplementary Material). 16 Finally, each nurse completed a 4-question survey at the end of the shift to describe how the scrubs worn during the shift compared to their standard work scrubs. Ultimately, each nurse answered 3 surveys, corresponding with each arm in the study.…”

Section: Methodsmentioning

confidence: 99%

The Antimicrobial Scrub Contamination and Transmission (ASCOT) Trial: A Three-Arm, Blinded, Randomized Controlled Trial With Crossover Design to Determine the Efficacy of Antimicrobial-Impregnated Scrubs in Preventing Healthcare Provider Contamination

Anderson

Addison

Lokhnygina

et al. 2017

Infect. Control Hosp. Epidemiol.

Self Cite

View full text Add to dashboard Cite

objective. To determine whether antimicrobial-impregnated textiles decrease the acquisition of pathogens by healthcare provider (HCP) clothing.design. We completed a 3-arm randomized controlled trial to test the efficacy of 2 types of antimicrobial-impregnated clothing compared to standard HCP clothing. Cultures were obtained from each nurse participant, the healthcare environment, and patients during each shift. The primary outcome was the change in total contamination on nurse scrubs, measured as the sum of colony-forming units (CFU) of bacteria.participants and setting. Nurses working in medical and surgical ICUs in a 936-bed tertiary-care hospital.intervention. Nurse subjects wore standard cotton-polyester surgical scrubs (control), scrubs that contained a complex element compound with a silver-alloy embedded in its fibers (Scrub 1), or scrubs impregnated with an organosilane-based quaternary ammonium and a hydrophobic fluoroacrylate copolymer emulsion (Scrub 2). Nurse participants were blinded to scrub type and randomly participated in all 3 arms during 3 consecutive 12-hour shifts in the intensive care unit.results. In total, 40 nurses were enrolled and completed 3 shifts. Analyses of 2,919 cultures from the environment and 2,185 from HCP clothing showed that scrub type was not associated with a change in HCP clothing contamination (P = .70). Mean difference estimates were 0.118 for the Scrub 1 arm (95% confidence interval [CI], −0.206 to 0.441; P = .48) and 0.009 for the Scrub 2 rm (95% CI, −0.323 to 0.342; P = .96) compared to the control. HCP became newly contaminated with important pathogens during 19 of the 120 shifts (16%).conclusions. Antimicrobial-impregnated scrubs were not effective at reducing HCP contamination. However, the environment is an important source of HCP clothing contamination. trial registration. Clinicaltrials.gov Identifier: NCT 02645214

show abstract

Pulse Field Gel Electrophoresis

Cited by 38 publications

References 22 publications

Clinical MRSA isolates from skin and soft tissue infections show increased in vitro production of phenol soluble modulins

Clinical MRSA isolates from skin and soft tissue infections show increased in vitro production of phenol soluble modulins

Molecular Epidemiology of Staphylococcus aureus Lineages in Wild Animals in Europe: A Review

The Antimicrobial Scrub Contamination and Transmission (ASCOT) Trial: A Three-Arm, Blinded, Randomized Controlled Trial With Crossover Design to Determine the Efficacy of Antimicrobial-Impregnated Scrubs in Preventing Healthcare Provider Contamination

Contact Info

Product

Resources

About