Pulsed Electric Fields Effects on Drying of White Ginseng and Extraction of Soluble Components

임정호,; 심재민,; 이동언,; 김영호,; 박기재,

doi:10.9721/kjfst.2012.44.6.704

Cited by 8 publications

(4 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high molecular weight ginsenosides, such as Re, can be specifically hydrolyzed to the low molecular weight ones through an ultra high-pressure process [42] . The study about PEF on the extraction of ginseng soluble components indicated that the soluble solid content can be increased by application of PEF, and the sugar and free sugar content of the extract is significantly reduced compared with non-PEF-treated samples [9] . Therefore, relatively prominent antioxidant activity of PEF extracts in our study was thought to be due to a different composition of ginsenosides from ginseng.…”

Section: Resultsmentioning

confidence: 99%

“…Pulsed electric field (PEF) has been used for the extraction of ginsenosides, which showed a higher yield than other common methods [8], [9]. Several studies have demonstrated that specific treatments, such as heat processing, can improve the medicinal efficacies of ginsenosides such as antioxidant and anticancer activities, and indicated that the improvement of biological activities was related to the structural change of ginsenosides by heat processing [10], [11], [12].…”

Section: Introductionmentioning

confidence: 99%

“…A variety of studies concentrate on yields of extractions and antioxidant mechanisms of individual ginsenoside; however, little is known about the underlying effect of different extraction methods on the biological activity of ginsenosides under the premise of higher yields. Pulsed electric field (PEF) has been used for the extraction of ginsenosides, which showed a higher yield than other common methods [8] , [9] . Several studies have demonstrated that specific treatments, such as heat processing, can improve the medicinal efficacies of ginsenosides such as antioxidant and anticancer activities, and indicated that the improvement of biological activities was related to the structural change of ginsenosides by heat processing [10] , [11] , [12] .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells

Liu

Zhang

Chen

et al. 2017

Journal of Ginseng Research

View full text Add to dashboard Cite

BackgroundGinsenosides are the main pharmacological components of Panax ginseng root, which are thought to be primarily responsible for the suppressing effect on oxidative stress.Methods2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) cells were incubated with ginsenosides extracted by pulsed electric field (PEF) and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced by 40μM H2O2. The cell viability and surface morphology of HEK-293 cells were studied using MTS assay and scanning electron microscopy, respectively. Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to measure the level of intracellular reactive oxygen species. The intracellular antioxidant activities of ginsenosides were evaluated by cellular antioxidant activity assay in HepG2 cells.ResultsThe PEF extracts displayed the higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen radical absorption capacity (with an oxygen radical absorption capacity value of 14.48 ± 4.04μM TE per μg/mL). The HEK-293 cell model also suggested that the protective effect of PEF extracts was dose-dependently greater than SCSE extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF extracts are more active (8% higher than SCSE extracts) in reducing intracellular reactive oxygen species accumulation. In addition, scanning electron microscopy images showed that the HEK-293 cells, which were treated with PEF extracts, maintained more intact surface morphology. Cellular antioxidant activity values indicated that ginsenosides extracted by PEF had stronger cellular antioxidant activity than SCSE ginsenosides extracts.ConclusionThe present study demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. Furthermore, rather than SCSE, PEF may be more useful as an alternative extraction technique for the extraction of ginsenosides with enhanced antioxidant activity.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells

Liu

Zhang

Chen

et al. 2017

Journal of Ginseng Research

View full text Add to dashboard Cite

show abstract

“…The effects of PEF on the drying of ginseng (at 55 ∘ C) and hot water (95 ∘ C) extraction of dried ginseng was studied [77]. PEF was applied to fresh ginseng using 1 and 2 kV cm −1 electric field strengths, 30 μs pulse duration, 25 and 200 Hz frequencies, and pulse number of 175.…”

Section: Ginsengmentioning

confidence: 99%