Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells

Lee, Mi Hee; Park, Ye Jin; Hong, Seok Jin; Koo, Min Ah; Cho, Minyoung; Park, Jong Chul

doi:10.3390/cells10112846

Cited by 12 publications

(13 citation statements)

References 51 publications

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“…In many studies, ES has been shown to have significant potential as a physical stimulus for activating intracellular signaling pathways involved in cell migration, proliferation, and differentiation. − Based on the results presented in Section , it appears that the PEDOT:PSS scaffold composites containing MXene (P1) exhibited greater osteoinductive potential compared to P0, as indicated by gene expression levels. This suggests that the addition of MXene to the scaffold may enhance its ability to promote osteogenesis.…”

Section: Resultsmentioning

confidence: 99%

Facilitation of Osteogenic Differentiation of hASCs on PEDOT:PSS/MXene Composite Sponge with Electrical Stimulation

Chen

Lin

Liu

et al. 2023

ACS Appl. Polym. Mater.

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Innovations in biomedical tissue engineering are on the increase as many researchers look for ways to develop biological scaffolds for cells. Studies have shown that the application of external force and electrical stimulation (ES) promotes stem cell chondrogenic and osteogenic differentiation. Therefore, bioscaffolds sensitive to external stimuli can not only influence the cell behavior with their native physical and biochemical properties but also act as a mediator receiving an outside-in signal to alter the cellular activities under specific conditions. For the first time, the synthetic polymer poly(3,4-ethylene dioxythiophene):polystyrene sulfonate) (PEDOT:PSS) was combined with a two-dimensional, nanoconducting material, titanium carbide (MXene, Ti 3 C 2 X 3 ), to prepare three-dimensional (3D) conductive scaffolds. Some studies have shown that MXene has good biocompatibility, osteoinductivity, and bone regeneration activity. The PEDOT:PSS/MXene scaffold was applied to the osteogenic differentiation of hASCs, and ES was used to enhance the osteogenic differentiation. The results showed that the conductive scaffold had low cytotoxicity to hASCs, which could grow and migrate in the 3D scaffold. In addition, osteogenic-specific gene expression significantly differed when the ES was applied. We propose that this PEDOT:PSS/MXene scaffold may serve as a platform for the study of osteogenic differentiation of stem cells with ES and may potentially be ex vivo fabricated as a tissue engineering construct for studying other modes of other ESs such as direct current, capacitive, or inductive coupling in a 3D environment.

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Section: Resultsmentioning

confidence: 99%

Facilitation of Osteogenic Differentiation of hASCs on PEDOT:PSS/MXene Composite Sponge with Electrical Stimulation

Chen

Lin

Liu

et al. 2023

ACS Appl. Polym. Mater.

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“…23.513372 doi: bioRxiv preprint effects of PEF on wound healing 8,15 . This PEF stimulation has been proved to be better than DC-EF in increasing cell migration speed, promoting cell migration persistence 16,17 , and additionally PEF-treated fibroblasts expressed higher levels of type I collagen further benefiting the wound healing 18 . However, hours of exposure in the current stimulation inevitably affects cell viability due to an electrochemical reaction and the Joule heat effect in the medium 19 .…”

Section: Introductionmentioning

confidence: 93%

Microsecond pulse electrical stimulation modulates cell migration

Xiang¹,

Liu²,

Liu³

et al. 2022

Preprint

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Wound healing is a complicated process for maintaining skin integrity after injury, for which electrical stimulations (ES) are ascribed to promote wound healing by facilitating cell migration. Time-shortening of the stimulation treatment from current hours to minutes for efficient wound healing but free of cell damage in return, is however rather a challenge. Here, a novel mechanism of ultrashort pulse electric filed (PEF), microsecond PEF at higher voltage, is proposed and realized to promote wound healing under a much short time (seconds) for the total treatment. We revealed that microsecond PEF regulated actin cytoskeleton reorganization and focal adhesion turnover, promoting fibroblasts migration in 2D cell cultures under the pulse stimulation. This accelerated fibroblast migration was accompanied by the mutual promotion with extracellular matrix (ECM) alignment in 3D microenvironments, which cooperatively benefit the eventual wound healing, and these findings were further confirmed by the enhanced skin wound healing in a classic mouse model. Additionally, we coined an actin- and collagen-dependent mechanism of microsecond PEF-mediated wound healing. The quantitative mechanism proposed here for our novel microsecond pulse electric field (μsPEF) methodology orients the new practical electric treatment in a wide range of biomedical applications, such as wound healing, regenerative medicine, and tissue engineering.

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“…Similarly, a PC, which can be a direct or alternating current, produces a unidirectional or bidirectional current, allowing a dynamic range of electrical frequency, strength, and duration [ 300 , 302 ]. Cellular directional migration during ES stimulation, otherwise known as electrotaxis or galvanotaxis, is influenced by the polarity of the activated intracellular signaling pathways and Golgi apparatus [ 300 , 302 , 303 ]. However, this phenomenon is cell-type dependent, with MSCs and iPSCs directed toward the anode and neural stem cell (NSC) migration toward the cathode [ 302 , 303 , 304 ].…”

Section: Neurotrophic Support In Neuropathy Treatmentmentioning

confidence: 99%

Effects of Physical Cues on Stem Cell-Derived Extracellular Vesicles toward Neuropathy Applications

Berry,

Ene,

Nathani

et al. 2024

Biomedicines

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The peripheral nervous system undergoes sufficient stress when affected by diabetic conditions, chemotherapeutic drugs, and personal injury. Consequently, peripheral neuropathy arises as the most common complication, leading to debilitating symptoms that significantly alter the quality and way of life. The resulting chronic pain requires a treatment approach that does not simply mask the accompanying symptoms but provides the necessary external environment and neurotrophic factors that will effectively facilitate nerve regeneration. Under normal conditions, the peripheral nervous system self-regenerates very slowly. The rate of progression is further hindered by the development of fibrosis and scar tissue formation, which does not allow sufficient neurite outgrowth to the target site. By incorporating scaffolding supplemented with secretome derived from human mesenchymal stem cells, it is hypothesized that neurotrophic factors and cellular signaling can facilitate the optimal microenvironment for nerve reinnervation. However, conventional methods of secretory vesicle production are low yield, thus requiring improved methods to enhance paracrine secretions. This report highlights the state-of-the-art methods of neuropathy treatment as well as methods to optimize the clinical application of stem cells and derived secretory vesicles for nerve regeneration.

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Pulsed Electrical Stimulation Enhances Consistency of Directional Migration of Adipose-Derived Stem Cells

Cited by 12 publications

References 51 publications

Facilitation of Osteogenic Differentiation of hASCs on PEDOT:PSS/MXene Composite Sponge with Electrical Stimulation

Facilitation of Osteogenic Differentiation of hASCs on PEDOT:PSS/MXene Composite Sponge with Electrical Stimulation

Microsecond pulse electrical stimulation modulates cell migration

Effects of Physical Cues on Stem Cell-Derived Extracellular Vesicles toward Neuropathy Applications

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