Pulsed electromembrane extraction for analysis of derivatized amino acids: A powerful technique for determination of animal source of gelatin samples

Rezazadeh, Maryam; Yamini, Yadollah; Seidi, Shahram; Aghaei, Ali

doi:10.1016/j.talanta.2015.01.007

Cited by 34 publications

(14 citation statements)

References 25 publications

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“…Rezazadeh et al (2015) introduced a pulsed electro membrane extraction method of amino acids coupled with high performance liquid chromatography-ultraviolet detector to differentiate bovine and porcine gelatins. Asn and Gln samples were derivatized with o-Phtalaldehyde (OPA) reagent to have UV absorbance and hydrophobicity/ lipophilicity prior to analysis; in addition, pulsed voltages were applied to make the analytes migrate through organic liquid membrane into an aqueous acceptor phase via hollow fiber-based liquid phase micro extraction method.…”

Section: Peme) Followed By Hplc-uv Methodsmentioning

confidence: 99%

Origin Determination and Differentiation of Gelatin Species of Bovine, Porcine, and Piscine through Analytical Methods

Eryılmaz

Işık

Demircan

et al. 2017

Turkish JAF Sci.Tech.

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Gelatin origin determination has been a crucial issue with respect to religion and health concerns. It is necessary to analyze the origin of gelatin with reliable methods to ensure not only consumer choices but also safety and legal requirements such as labeling. There are many analytical methods developed for detection and/or quantification of gelatin from different sources including bovine, porcine and piscine. These analytical methods can be divided into physicochemical, chromatographic, immunochemical, spectroscopic and molecular methods. Moreover, computational methods have been used in some cases consecutively to ensure sensitivity of the analytical methods. Every method has different advantages and limitations due to their own principles, applied food matrix and process conditions of material. The present review intends to give insight into novel analytical methods and perspectives that have been developed to differentiate porcine, bovine and piscine gelatins and to establish their authenticity. Almost every method can be succeeded in origin determination; however, it is a matter of sensitivity in that some researches fail to ensure sufficient differentiation. IntroductionGelatin is a water soluble protein obtained by hydrolysis of collagen to several high molecular weight polypeptides. While the main component of the gelatin is protein (85-92%), it also contains mineral salts and moisture (Baziwane and He, 2003;Schrieber and Gareis, 2007). Gelatin has a great importance in pharmaceutical, cosmetic and especially food industry with its well-known functional properties such as gel formation in jellies, foam formation and stabilization in ice cream, emulsion stabilization in meat products, syneresis stabilization in yogurt (Nhari et al., 2012). Gelatin was produced approximately 326.000 metric tons in 2008 worldwide, and it is estimated to be consumed 395.840 metric tons by the year of 2017 according to Global Industry Analysts, Inc. which is one of the largest and reputed market research companies (Anonymous, 2016).The raw materials used in gelatin production are mainly from bovine and porcine sources: 80% from pig skin, 15% from cattle hide split, the remaining 5 % from bones, fish and other sources (GME, 2016). Gelatin origin determination has been a matter of paramount importance for Muslims, Hindus, Jews, vegetarians and vegans, in that, in Islam and Judaism, porcine derivatives are prohibited to consume, whereas in Hinduism, cow can neither be slaughtered nor consumed. Vegetarians and vegans prefer to eat animal-free foods (Boran et al., 2010;Nhari et al., 2012). Moreover, bovine gelatin has been restricted for human consumption due to Bovine Spongiform Encephalopathy (BSE) outbreak in 1986 in England and other countries (Venien and Levieux, 2005). Therefore, it is necessary to analyze the origin of gelatin with a reliable method. As far as it is concerned; physiochemical, chromatographic, immunochemical, spectroscopic and molecular methods have been developed to determine and differentiate gelatin o...

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Section: Peme) Followed By Hplc-uv Methodsmentioning

confidence: 99%

Origin Determination and Differentiation of Gelatin Species of Bovine, Porcine, and Piscine through Analytical Methods

Eryılmaz

Işık

Demircan

et al. 2017

Turkish JAF Sci.Tech.

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“…The majority of the EME and EE work was applied to drug compounds or relatively hydrophobic compounds, such as aromatic amino acids [21], azo red dyes [22], herbicides [23], and volatile organic compound metabolites [24,25]. Notable exceptions are the EME of inorganic anions [26], pulsed mode EME of histidine, phenylalanine, tryptophan [21], and asparagine and glutamine [27]. Not all papers included in the table reported both ER and EF.…”

Section: Hyphenation Of Eme and Ee: An Overviewmentioning

confidence: 99%

Electroextraction and electromembrane extraction: Advances in hyphenation to analytical techniques

et al. 2016

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Electroextraction (EE) and electromembrane extraction (EME) are sample preparation techniques that both require an electric field that is applied over a liquid‐liquid system, which enables the migration of charged analytes. Furthermore, both techniques are often used to pre‐concentrate analytes prior to analysis. In this review an overview is provided of the body of literature spanning April 2012–November 2015 concerning EE and EME, focused on hyphenation to analytical techniques. First, the theoretical aspects of concentration enhancement in EE and EME are discussed to explain extraction recovery and enrichment factor. Next, overviews are provided of the techniques based on their hyphenation to LC, GC, CE, and direct detection. These overviews cover the compounds and matrices, experimental aspects (i.e. donor volume, acceptor volume, extraction time, extraction voltage, and separation time) and the analytical aspects (i.e. limit of detection, enrichment factor, and extraction recovery). Techniques that were either hyphenated online to analytical techniques or show high potential with respect to online hyphenation are highlighted. Finally, the potential future directions of EE and EME are discussed.

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“…This new micro‐extraction method was nominated electromembrane extraction (EME). During past years, the number of EME‐based analytical methods has been considerably increased and a great number of papers have been published to extend the fundamentals and applications of this microextraction method for the determination of different inorganic and organic compounds .…”

Section: Introductionmentioning

confidence: 99%

A novel method for the preconcentration and determination of ampicillin using electromembrane microextraction followed by high‐performance liquid chromatography

Aghaei

Jazi

Mlsna

et al. 2019

J of Separation Science

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Electromembrane extraction was used with high‐performance liquid chromatography for preconcentration and determination of ampicillin residues in cow milk. Ampicillin is transferred from an aqueous solution through a thin layer containing octan‐1‐ol, silver nanoparticles, and reduced graphene oxide which serves as a supported liquid membrane. Inside the fiber impregnated with supported liquid membrane mixture was filled 10 µL of an acceptor phase. Experimental parameters were optimized for extraction efficiency of ampicillin. Under the optimized conditions, the proposed method provided acceptable linear range (2–100 µg/L), satisfactory repeatability (RSD% < 7.1), low limit of detection (0.6 µg/L), and a high enrichment factor (295) corresponding to extraction recovery of 37%. Consequently, the proposed method was successfully applied for the determination of ampicillin residues in different cow milks.

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Pulsed electromembrane extraction for analysis of derivatized amino acids: A powerful technique for determination of animal source of gelatin samples

Cited by 34 publications

References 25 publications

Origin Determination and Differentiation of Gelatin Species of Bovine, Porcine, and Piscine through Analytical Methods

Origin Determination and Differentiation of Gelatin Species of Bovine, Porcine, and Piscine through Analytical Methods

Electroextraction and electromembrane extraction: Advances in hyphenation to analytical techniques

A novel method for the preconcentration and determination of ampicillin using electromembrane microextraction followed by high‐performance liquid chromatography

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