PuraMatrix™ Facilitates Bone Regeneration in Bone Defects of Calvaria in Mice

Misawa, Haruo; Kobayashi, Naoya; Soto-Gutiérrez, Alejandro; Chen, Yong; Yoshida, Aki; Rivas‐Carrillo, Jorge David; Navarro-Alvarez, Nalú; Tanaka, Kazunori; Miki, Atsushi; Takei, Jiro; Ueda, Tomoya; Tanaka, Masato; Endo, Hidenori; Tanaka, Noriaki; Ozaki, Toshifumi

doi:10.3727/000000006783981369

Cited by 109 publications

(89 citation statements)

References 33 publications

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“…Therefore, injectable strategies using polysaccharide-based hydrogels are being explored. Hydrogels fabricated from fibrin, 44 fibronectin, 45 or Matrigel 46 have favorable characteristics of being injectable and having peptide domains that facilitate cellular attachment for bone tissue regeneration applications. However, these peptide-based hydrogels are slow to solidify and have the potential to efflux from the site of bone defect.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Multifunctional Polysaccharide Hydrogels with Varying Stiffness for Bone Tissue Engineering

Pandit

Zuidema

Venuto

et al. 2013

Tissue Engineering Part A

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The use of hydrogels for bone regeneration has been limited due to their inherent low modulus to support cell adhesion and proliferation as well as their susceptibility to bacterial infections at the wound site. To overcome these limitations, we evaluated multifunctional polysaccharide hydrogels of varying stiffness to obtain the optimum stiffness at which the gels (1) induce proliferation of human dermal fibroblasts, human umbilical vascular endothelial cells (HUVECs), and murine preosteoblasts (MC3T3-E1), (2) induce osteoblast differentiation and mineralization, and (3) exhibit an antibacterial activity. Rheological studies demonstrated that the stiffness of hydrogels made of a polysaccharide blend of methylcellulose, chitosan, and agarose was increased by crosslinking the chitosan component to different extents with increasing amounts of genipin. The gelation time decreased (from 210 to 60 min) with increasing genipin concentrations. Proliferation of HUVECs decreased by 10.7 times with increasing gel stiffness, in contrast to fibroblasts and osteoblasts, where it increased with gel stiffness by 6.37 and 7.8 times, respectively. At day 14 up to day 24, osteoblast expression of differentiation markers-osteocalcin, osteopontin-and early mineralization marker-alkaline phosphatase, were significantly enhanced in the 0.5% (w/v) crosslinked gel, which also demonstrated enhanced mineralization by day 25. The antibacterial efficacy of the hydrogels decreased with the increasing degree of crosslinking as demonstrated by biofilm formation experiments, but gels crosslinked with 0.5% (w/v) genipin still demonstrated significant bacterial inhibition. Based on these results, gels crosslinked with 0.5% (w/v) genipin, where 33% of available groups on chitosan were crosslinked, exhibited a stiffness of 502 -64.5 Pa and demonstrated the optimal characteristics to support bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Multifunctional Polysaccharide Hydrogels with Varying Stiffness for Bone Tissue Engineering

Pandit

Zuidema

Venuto

et al. 2013

Tissue Engineering Part A

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“…We have used Puramatrix (Bioscience, San Jose, CA, USA), a peptide matrix composed by multiple sequences of arginine (R), alanine (A), aspartate (D) and alanine (A). This scaffold has shown to promote cell growth and differentiation (59)(60)(61). In our initial experiments with this scaffold, it has shown compatibility with DPSC allowing them to grow and differentiate (Fig.…”

Section: Scaffolds For Dental Pulp Tissue Engineeringmentioning

confidence: 99%

Dental pulp tissue engineering

et al. 2011

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Dental pulp is a highly specialized mesenchymal tissue that has a limited regeneration capacity due to anatomical arrangement and postmitotic nature of odontoblastic cells. Entire pulp amputation followed by pulp space disinfection and filling with an artificial material cause loss of a significant amount of dentin leaving as life-lasting sequelae a non-vital and weakened tooth. However, regenerative endodontics is an emerging field of modern tissue engineering that has demonstrated promising results using stem cells associated with scaffolds and responsive molecules. Thereby, this article reviews the most recent endeavors to regenerate pulp tissue based on tissue engineering principles and provides insightful information to readers about the different aspects involved in tissue engineering. Here, we speculate that the search for the ideal combination of cells, scaffolds, and morphogenic factors for dental pulp tissue engineering may be extended over future years and result in significant advances in other areas of dental and craniofacial research. The findings collected in this literature review show that we are now at a stage in which engineering a complex tissue, such as the dental pulp, is no longer an unachievable goal and the next decade will certainly be an exciting time for dental and craniofacial research.

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“…PuraMatrix is a repeating polymer of the amino acid sequence R-A-D-A and is formulated as an injectable scaffold that assembles into nanofibers when exposed to physiologic concentrations of salts. This scaffold has been used in several studies and has yielded promising results for cardiac, [8][9][10][11] neural, 12,13 hepatic, 14 and bone 15 tissue regeneration. Furthermore, PuraMatrix has been shown to be capable of maintaining the viability and odontogenic differentiation capacity of DPSCs.…”

Section: Introductionmentioning

confidence: 99%