Purcell factor based understanding of enhancements in surface plasmon-coupled emission with DNA architectures

Abstract

We demonstrate the successful application of DNA thin films as dynamic bio-spacers in a surface plasmon-coupled emission platform. Site-directed DNA modification using silver and carbon nanomaterials resulted in an amplified Purcell factor (PF) and >130-fold fluorescence enhancements. We present unique architectures of DNA as a plasmonic spacer in metal-dielectric-metal substrates.

Silver Soret Nanoparticles for Femtomolar Sensing of Glutathione in a Surface Plasmon-Coupled Emission Platform

“…In line with the earlier reports that elucidate the utility of FDTD simulations in understanding fluorescence enhancements from cermet nanocavities, Purcell-enhanced emission from metal–DNA hybrid architectures, we have successfully comprehended the plasmonic response from SCs on a silver thin film using FDTD. Earlier reports have systematically deduced the effect of the cavity regions on the PF adopting cubic and spherical NPs .…”

“…In the current study, the TEM and SEM images showcase such anisotropic clustering of AgNPs to yield multifaceted SCs, making them ideal candidates for study using the SPCE platform. Although there are several templated nanomaterials used in cavity architecture depicting their plasmonic response, − the PF as an experimental metric for understanding SPCE enhancements has to date been reported only for intermetallics . In this scenario, understanding and comparing theoretical conclusions drawn using FDTD simulations with the results documented in the previous sections will provide for better insight.…”

“…Additionally, researchers have also utilized spacer layers in applications pertaining to the plasmon-enhanced solar membrane distillation of seawater and smartphone-based surface-enhanced fluorescence for point-of-care diagnostics in resource-limited settings . In this regard, a variety of spacer materials have been exploited on the SPCE platform. ,, In this report, the Ag-SCs are sandwiched between the silver thin film and fluorescent molecules (Rh6G) acting as new spacer nanomaterials. The SPCE enhancements obtained because of the effect of Ag-SCs spacers are provided in Figure a.…”

“…A decrease in the fluorescence lifetime, an increase in the radiative decay rate, enhanced quantum yield, and high PF accompany such phenomenon because of the creation of cavity hotspots . Spontaneous emission from a radiating dipole is directly affected by the cavity environment and is known as the Purcell effect. ,, …”

“…These modifications were viable for biosensing applications such as dopamine and tryptophan sensing at very low limits of detection (LODs). Dissimilar materials1D and 2D carbon substrates, cermet nanocavities, gold-decorated SiO 2 nanohybrids, DNA thin films, and palladium–carbon plasmonic nanocavities in the spacer and cavity configurations has led to interesting advances in plasmonics with wide applications. In this regard, it is to be noted that the use of AgNPs assembled/decorated on different nanotemplates, such as carbon nanotube (CNT), graphene, protein, and low-dimensional carbon, resulted in amplified emission enhancements on the SPCE platform.…”