Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila

Levanova, Nadezhda; Tabakova, Irina; Jank, Thomas; Belyi, Yury

doi:10.1007/978-1-4939-9048-1_18

Cited by 1 publication

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“…Lgt1 (Lpg1368, WP_010947098.1) was the first (and, to our knowledge, the only) Legionella T4BSS effector directly isolated from Legionella culture [ 33 , 57 ]. It was identified by NH 2 -terminal amino acid sequencing of the protein purified from L. pneumophila cells and consisted of 525 amino acid residues with a molecular mass of 60 kDa and an isoelectric point of 7.0 [ 33 ].…”

Section: L Pneumophila Glucosyltransferases 1-3 (Lgt1-3)mentioning

confidence: 99%

Glycosylating Effectors of Legionella pneumophila: Finding the Sweet Spots for Host Cell Subversion

Belyi

Levanova²,

Schroeder

2022

Biomolecules

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Work over the past two decades clearly defined a significant role of glycosyltransferase effectors in the infection strategy of the Gram-negative, respiratory pathogen Legionella pneumophila. Identification of the glucosyltransferase effectors Lgt1-3, specifically modifying elongation factor eEF1A, disclosed a novel mechanism of host protein synthesis manipulation by pathogens and illuminated its impact on the physiological state of the target cell, in particular cell cycle progression and immune and stress responses. Recent characterization of SetA as a general O-glucosyltransferase with a wide range of targets including the proteins Rab1 and Snx1, mediators of membrane transport processes, and the discovery of new types of glycosyltransferases such as LtpM and SidI indicate that the vast effector arsenal might still hold more so-far unrecognized family members with new catalytic features and substrates. In this article, we review our current knowledge regarding these fascinating biomolecules and discuss their role in introducing new or overriding endogenous post-translational regulatory mechanisms enabling the subversion of eukaryotic cells by L. pneumophila.

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Section: L Pneumophila Glucosyltransferases 1-3 (Lgt1-3)mentioning

confidence: 99%