Purification and characterization of a lectin from wild sunflower (Helianthus tuberosus L.) tubers

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“…Earlier studies presented the wild lectins viz. Glycine soja (Pueppke et al, 1982), Acacia constricta (Guzmán-Partida et al, 2004), Helianthus tuberosus L. (Suseelan et al, 2002), Bradyrhizobium japonicum (Larry and Gary, 1986), Boletus edulis (Zheng et al, 2007;Singh et al, 2004), Phaseolus vulgaris L. (Sotelo et al, 1994) have been reported isolated and characterised subsequently.…”

Helianthus tuberosus

“…Heltuba was found to be a cytosolic, tetrameric protein composed of four identical subunits of 15.5 kDa and exhibited a preferential specifi city towards oligomannosides . Another lectin (HTTL) with native molecular mass of 72 kDa and subunit molecular masses of 17 and 18.5 kDa was isolated from Helianthus tuberosus tubers (Suseelan et al 2002 ). The lectin agglutinated both untreated and trypsin-treated rabbit erythrocytes and did not agglutinate human blood cells of groups A, B and O.…”

Affinity Chromatography of Lectins

“…Recently, isolation of lectins from various sources has been described using numerous sugars, such as lactose (Almanza et al, 2004;Braga et al, 2006;Fujii et al, 2011;Hamako et al, 2007;Kawsar et al, 2009;Mendonça-Franqueiro et al, 2011;Naeem et al, 2007b;Pohleven et al, 2009;Rocha et al, 2009;Silva et al, 2007), sucrose and glucose (Pohleven et al, 2011), galactose (Konami et al, 1991;Lavanya Latha et al, 2006;Mo et al, 2000;Moreira et al, 1998;Naeem et al, 2007b;Nagata, 2005), mannose (Andon et al, 2003;Ooi et al, 2010;Suseelan et al, 2002), fucose (Cammarata et al, 2007;Mansour & Abdul-Salam, 2009), L-rhamnose (Jimbo et al, 2007;Watanabe et al, 2008), and sugars with 2'-acetamido groups N-acetylgalactosamine (Gerlach et al, 2005;Perçin et al, 2009;Qureshi et al, 2006), N-acetylglucosamine (Kaur et al, 2005;Kim et al, 2006;Maheswari et al, 2002;Wang & Ng, 2003), and N,N'-diacetylchitobiose (Konami et al, 1991;Koyama et al, 2002).…”

“…Commercially available agarose matrices pre-coupled with carbohydrates or glycoproteins have also been used, such as agarose beads with immobilized lactose (EY Laboratories) (Hamako et al, 2007), galactose (Pierce) (Trindade et al, 2006), mannose (Sigma-Aldrich) (Ooi et al, 2002;Suseelan et al, 2002), N-acetylgalactosamine (Gerlach et al, 2005;Qureshi et al, 2006), N-acetylglucosamine (Sigma-Aldrich) (Kim et al, 2006;Wang & Ng, 2003), N,N'-diacetylchitobiose (Sigma-Aldrich) (Koyama et al, 2002), mannan (Sigma-Aldrich) (Naeem et al, 2007a;Ourth et al, 2005), and fetuin (Sigma-Aldrich) (Ooi et al, 2002;Rittidach et al, 2007).…”

“…Several authors report the elution of lectins using acidic (20-100 mM glycine-HCl or ǐ-alanine buffer, pH ~2.5, or 1-3 % acetic acid) or alkaline (10 mM NaOH, 0.1 M triethanolamine buffer, pH 11, or Tris-OH buffer, pH 11.4) solutions as well as buffers containing 1 M NaCl or 3 M MgCl 2 (gradient was also used) (Table 1). In some cases, lectins have been eluted using 6 M or 8 M urea (Suseelan et al, 2002). The carbohydrate binding activity of some lectins, such as C-type lectins, depends on divalent metal ions (Ca 2+ ), therefore can be eluted using buffers containing chelating agents, such as 2-100 mM ethylenediaminetetraacetic acid (EDTA) ( …”