Purification and characterization of a novel high molecular weight alkaline protease produced by an endophytic Bacillus halotolerans strain CT2

Gharbi, Dorra; Karkouch, Ines; Imen, Ben Slimene; Coquet, Laurent; Azaiez, S.; Tabbene, Olfa; Cosette, Pascal; Thierry, Jouenne; Limam, Férid

doi:10.1016/j.ijbiomac.2018.01.024

Cited by 53 publications

(36 citation statements)

References 35 publications

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“…On the other hand, for the isolate B. thuringiensis MB-3 the maximum protease activity was achieved at 50 and 60 °C. In agreement with these results, maximum activity at 50 or 60 °C was also observed for several other proteases produced by Bacillus strains (Qureshi et al, 2018;Dorra et al, 2018;Bashir et al, 2018).…”

Section: Discussionsupporting

confidence: 86%

Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29

Devaraj

Aathika

Periyasamy

et al. 2018

Natural Product Research

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A strain of Bacillus amyloliquefaciens KUB29 was identified by 16S ribosomal RNA sequencing (Genbank: MF772779.1). Production of thermostable protease, amylase and lipase were done by the isolated strain. The produced enzymes were partially purified by ammonium precipitation followed by dialysis process. Protease and lipase enzymes are effectively used in bio-oil extraction from proteinaceous sample followed by transesterification to produce methyl ester. Amylase enzyme is widely used in food and laundry industry. The produced enzymes are active at thermophilic condition of 55 °C. Use of these enzymes in biofuel production process will make the process cleaner and greener.

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Section: Discussionsupporting

confidence: 86%

Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29

Devaraj

Aathika

Periyasamy

et al. 2018

Natural Product Research

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“…These results are in accordance with several previous reports showing optimal activity at alkaline pH (between 8 and 12) for alkaline proteases from micro-organisms (Nadeem et al 2013). Dorra et al (2018) identified Bacillus halotolerans, a protease-producing strain CT2 isolated from Tunisian potatoes, which exhibits an optimal protease activity at pH 9Á0. Microbial proteases have attracted considerable attention and account for approximately 60% of the total enzyme market (Cheng et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Biotechnological applications of the medicinal plant Pseudobrickellia brasiliensis and its isolated endophytic bacteria

Cardoso

Campos

Santos

et al. 2020

J. Appl. Microbiol.

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Aim This study aimed to isolate Pseudobrickellia brasiliensis endophytic bacteria and evaluate the production of hydrolytic enzymes and antibiotics by these bacterial strains. The study also measured the antibacterial activity of P. brasiliensis. Methods and Results Thirteen endophytic bacteria strains were isolated from stem and leaf fragments of P. brasiliensis. Extracellular enzyme production by the isolated endophytic bacteria was evaluated in an agar plate‐based assay. The highest protease production was achieved by Bacillus subtilis P4 in alkaline medium. Antimicrobial activity of endophytic bacteria and P. brasiliensis extracts was investigated using microbroth dilution. An MIC value of 1000 μg ml−1 against Pseudomonas aeruginosa was found for B. subtilis P3, B. subtilis P5, Pseudomonas sp. P8 and Pseudomonas sp. P12. Leaf extract of P. brasiliensis showed the highest antibacterial activity against P. aeruginosa, with an MIC value of 0·781 mg ml−1. Conclusions Pseudobrickellia brasiliensis is a source of bacterial endophytes, which can produce antibacterial compounds and enzymes. This work also demonstrated the antibacterial potential of P. brasiliensis. Significance and Impact of the Study This is the first study that revealed the antibacterial activity of P. brasiliensis and bioactive metabolite production by P. brasiliensis endophytic bacteria.

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“…B. licheniformis NH1 generated protease with 100% activity at 60 °C (El Hadj-Ali et al, 2007). Bacillus halotolerants strain CT2 showed maximum protease activity at pH 9 (Dorra et al, 2018). Protease from Bacillus subtilis AKAL7 and Exigubacterium indicum AKAL11showed similar pH activity (Hakim et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Proteases may be classified by the optimal pH in which they are active: Acidic proteases, Neutral proteases and Basic proteases (or Alkaline proteases) (Dorra et al, 2018). Alkaline proteases possess wide application in industrial plans.…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of alkaline protease-producing bacterium in the presence of washing-powder from Geinarje hot spring, Ardabil, Iran

Taheri

Ebrahimipour

Sadeghi

2019

nbr

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Proteases are important industrial enzymes used in different areas of industry, mainly detergent, food and leather industries. In this study, novel alkaline protease-producing bacterium was isolated from Geinarje hot spring and examined for maximum protease activity to be utilized in washing-powder. The isolated bacterium was cultured in mineral salt medium including 2% Skim Milk. Proteolytic activity of supernatant was measured by caseinolytic method. The effects of pH, temperature, SDS, Tween 80 and EDTA on protease stability and activity were investigated. The detergent compatibility of protease was assayed. On the basis of phylogenetic analysis and morphological as well as biochemical tests, the isolate was identified as a new strain of Brevibacillus borstelensis capable of generating extracellular alkaline protease. The generated protease was determined as alkaline metallo-protease having high activity at 60 o C and pH 9. Moreover, the alkaline protease was stable in the presence of SDS, Tween 80 and H2O2. It is compatible with commercial detergents. Finding proteases capable of degrading proteins in extreme environment (i.e. alkaline pH, high temperature and presence of surfactants) is valuable in biotechnological and industrial practices. Therefore, it can be utilized in detergent formulation in the future.

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Purification and characterization of a novel high molecular weight alkaline protease produced by an endophytic Bacillus halotolerans strain CT2

Cited by 53 publications

References 35 publications

Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29

Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29

Biotechnological applications of the medicinal plant Pseudobrickellia brasiliensis and its isolated endophytic bacteria

Isolation and identification of alkaline protease-producing bacterium in the presence of washing-powder from Geinarje hot spring, Ardabil, Iran

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