Purification and characterization of a protease from solid state cultures of Aspergillus parasiticus

Tunga, Rashbehari; Shrivastava, Binita; Banerjee, Rintu

doi:10.1016/s0032-9592(03)00048-7

Cited by 100 publications

(87 citation statements)

References 16 publications

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“…This suggested that the bacterial strain was alkaliphillic in nature and optimum pH range between 9.0 and 10.0 for growth and enzymes production is a common feature among alkaliphillic organisms (Fig. 3) [11].…”

Section: Effect Of Incubation Period (Ph 70; 37°c)mentioning

confidence: 93%

“…Alkaline proteases have wide-scale industrial applications including food processing, leather processing as a dehairing agent, textile industry, diagnostic reagents, household waste management, recovery of silver from X-ray film, and bioremediation [9,10]. Proteases have wide applications in many industries, namely, detergents, and food processing, especially for cheese ripening, meat tenderization, animal nutrition, pharmaceutics, paper industry, and food industry [11].…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Purification, and Optimization of Thermophilic and Alkaliphilc Protease Originating From Hot Water Spring Bacteria

Puntambekar

Dake

2017

Asian J Pharm Clin Res

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Objective: The main objective of this study is to investigate the industrial applications of a thermophillic alkaline protease from a hot water spring bacterial isolate "A" and to study its production, optimization, and purification. Methods:The alkaline protease was produced using shake flask studies maintaining a pH of 9.0 and a temperature of 50°C. Optimization studies of the enzyme were carried out using variable pH, temperature, organic carbon, and nitrogen sources followed by purification of the enzyme using DEAE-cellulose ion exchange chromatography technique. Stability of the enzyme was analyzed in the presence of organic solvents and surfactants. The efficiency of the enzyme in the removal of proteinaceous stains in the presence of strong detergents under extreme conditions was assessed. The fibrinolytic activity of the enzyme in dissolving the blood clot was confirmed. Results:The isolated alkaline protease was purified to homogeneity with a 16-fold increase. Media optimization studies revealed that 1% glucose and 1 % casein-induced the production of alkaline protease. The purified enzyme retained stability in the presence of ethanol, methanol, and acetone and surfactants such as 0.5% (w/v) sodium dodecyl sulfate (SDS) and 0.5% (v/v) Triton-X-100. The isolated alkaline protease successfully removed the proteinaceous stains and showed significant results in the dissolution of blood clot. Conclusion:The above experimental results confirm that the isolated enzyme has both thermophilic and alkaliphilic protease properties. Thereby the enzyme finds promising industrial applications even in extreme conditions.

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Section: Effect Of Incubation Period (Ph 70; 37°c)mentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Isolation, Purification, and Optimization of Thermophilic and Alkaliphilc Protease Originating From Hot Water Spring Bacteria

Puntambekar

Dake

2017

Asian J Pharm Clin Res

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“…Proteases known as proteinases or proteolytic enzymes which occur naturally in all organisms, they act on the peptide bonds formed by specific amino acids to hydrolyze them [7]. They represent one of the three largest groups of industrial enzymes and find application in detergents, leather industry, food industry; pharmaceutical industry and bioremediation processes [8].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Action of Streptomyces griseolus Proteases as Bio-Control on Fasciola gigantic Eggs

Gammal¹,

Shalaby²

2014

J Bacteriol Parasitol

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This work evaluated the in vitro bio-control action of actinomycetes isolated from Egyptian soil against Fasciola gigantica eggs. In this respect, the identified Streptomyces griseolus was the best parasite-control agent which gave the highest mortality percent applying three methods; the first method was the application of the grown bacterial disks with the eggs at zero time on the medium (2% w/v water-agar) and incubated for 21 days (75.5% mortality), the second method was the incubation of the bacterial disks using the same medium for 5 days before eggs inoculation, after 21 days of incubation the mortality percent was 80.8%. The application of the third method showed a good effect by using all bacterial culture filtrates. S. griseolus filtrate which had the highest lytic enzyme and proteolytic activities reached 2.0 and 660.7 U/ml, respectively, gave the highest mortality (95.5%) of F. gigantica eggs compared to the other bacterial filtrates. The crude culture filtrate and the diluted culture filtrate (1.33 fold) of S. griseolus gave the highest mortality percent against the parasite´s eggs (95.8 and 94.5%, respectively) compared to the higher diluted filtrates. The culture filtrate of S. griseolus grown on two different media and its partially purified enzyme (precipitated by 60% ammonium sulfate) were tested for their proteolytic activity and inhibitory effect against F. gigantica eggs. Medium no. 2 showed the highest proteolytic activity (1030.3 and 1138.1 U/ml) for the crude and the partially purified enzyme, respectively. While the highest inhibitory effect reached 97.6 and 89.9%, respectively. Finally, the application of crude enzyme filtrate was better than the partially purified one. Streptomyces griseolus was proved as a potential biological control agent for this helminth.

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“…They account for 65% of total world wide enzyme sale [1]. They have major applications in industrial process such as laundry detergents, leather, silk, pharmaceutical, food and degradation of gelatin on X-ray films [1,2,3,4]. They are also used in bioremediation process [5].…”

Section: Introductionmentioning

confidence: 99%

Kinetic study of free and immobilized protease from Aspergillus sp

Sharma¹

2013

IOSR-JPBS

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Purification and characterization of a protease from solid state cultures of Aspergillus parasiticus

Cited by 100 publications

References 16 publications

Isolation, Purification, and Optimization of Thermophilic and Alkaliphilc Protease Originating From Hot Water Spring Bacteria

Isolation, Purification, and Optimization of Thermophilic and Alkaliphilc Protease Originating From Hot Water Spring Bacteria

In Vitro Action of Streptomyces griseolus Proteases as Bio-Control on Fasciola gigantic Eggs

Kinetic study of free and immobilized protease from Aspergillus sp

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