1988
DOI: 10.1128/jb.170.4.1789-1794.1988
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Purification and characterization of a bacterial nitrophenol oxygenase which converts ortho-nitrophenol to catechol and nitrite

Abstract: A nitrophenol oxygenase which stoichiometricaly converted ortho-nitrophenol (ONP) to caitechol and nitrite was isolated from Pseudomonas putida B2 and purified. The substrate specificity of the enzymie was broad and included several halogen-and alkyl-substituted ONPs. The oxygenase consisted of a single polypeptide chain with a molecular weight of 58,000 (determined by gel filtration) or 65,000 (determinedon a sodium dodecyl sulfate-polyacrylamide gel). The enzymatic reaction was NADPH dependent, and one hiole… Show more

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Cited by 82 publications
(87 citation statements)
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“…Pseudomonas putida B2 grows on both 2-and 3-nitrophenol, but by use of different pathways (215). 2-Nitrophenol is oxidized by a NADPH-dependent monooxygenase through o-benzoquinone to produce nitrite and catechol (216), which is further metabolized by the ␤-ketoadipate pathway (215, 217) ( Fig. 9 and 10).…”
Section: Pathways For Nitrophenol Catabolismmentioning
confidence: 99%
“…Pseudomonas putida B2 grows on both 2-and 3-nitrophenol, but by use of different pathways (215). 2-Nitrophenol is oxidized by a NADPH-dependent monooxygenase through o-benzoquinone to produce nitrite and catechol (216), which is further metabolized by the ␤-ketoadipate pathway (215, 217) ( Fig. 9 and 10).…”
Section: Pathways For Nitrophenol Catabolismmentioning
confidence: 99%
“…A partially purified THT oxygenase catalyzed ring fission of THT and accumulation of a compound tentatively identified as 3-hydroxy-5-(1-formylethylidene)-2-furanone. Preliminary results indicate that this compound is an artifact of the isolation procedure and suggest that 2,4-dihydroxy-5-methyl-6-oxo-2,4-hexadienoic acid is the actual ring fission product.Microorganisms can directly remove nitro groups from nitroaromatic compounds by either oxidative routes (6,12,16,17,22) or reductive routes (7,11). Both monooxygenase (14,22), and dioxygenase (6, 12, 17) enzyme systems can catalyze the elimination of nitrite from nitroaromatic compounds.…”
mentioning
confidence: 99%
“…Microorganisms can directly remove nitro groups from nitroaromatic compounds by either oxidative routes (6,12,16,17,22) or reductive routes (7,11). Both monooxygenase (14,22), and dioxygenase (6,12,17) enzyme systems can catalyze the elimination of nitrite from nitroaromatic compounds.…”
mentioning
confidence: 99%
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“…This mechanism Isolation and characterization of organisms is observed in microorganisms that degrade mono-and dinitro-A bacterial strain with the capacity to utilize anthranilate as the substituted toluenes (Robertson et al 1992;Spain et al 1979; sole carbon and energy source was isolated from sludge col- Spanggord et al 1991;Valli et al 1992;Zeyer and Kocher lected at a solid waste treatment facility (Alvarez 1993). The 1988).…”
Section: Methodsmentioning
confidence: 99%