Purification and characterization of chitin-binding proteins from the hemolymph of sweet potato hornworm, Agrius convolvuli

Identification of the chitin-binding proteins from the larval proteins of silkworm, Bombyx mori

“…This analysis allowed for identification of peptides of two lysozyme precursors that have been annotated from Bombyx genome. This result is not surprising given that lysozyme has been shown to be a chitin-binding protein (Chae et al, 1999). In this study, a apolipophorin III, a 30 kDa lipoprotein, and a promoting protein were also identified.…”

Molecular characterization of the modular chitin binding protein Cbp50 from Bacillus thuringiensis serovar konkukian

“…g-i Hyphal inhibition assay for A. niger, g is control; h, i represents Cbp-50 protein and CBD-N, respectively systems (Bormann et al 1999;Selitrennikoff 2001). Chitin-binding proteins and peptides with antifungal activity have been isolated from a number of sources, including bacteria (Bormann et al 1999;Kolbe et al 1998), plants, insects, and crustaceans (Chae et al 1999;De Bolle et al 1993;Huang et al 2000;Nielsen et al 1997;Osborn et al 1995). Preliminary assays of Cbp50 and CBD-N have shown that both have antifungal activity against pathogenic fungi (Fig.…”

Antifungal Proteins

“…Chitin-binding proteins and peptides that have antifungal activity but are not PR proteins have been isolated from a number of sources, including bacteria (13), plants, insects, and crustaceans (19,29,61,76,83,131,136). These non-PR-4 chitin-binding proteins include the tachystatins (75, 135) (horseshoe crab, 6.8 to 7.4 kDa), the penaeidins (31-33) (penaeid shrimp, 5.5 to 6.6 kDa), antifungal protein 1 (AFP1) (13) (Streptomyces tendae, 9.8 kDa), and others.…”