Purification and characterization of RGA2, a Rho2 GTPase-activating protein from Tinospora cordifolia

Abstract

Rho GTPases activating protein 2 (RGA2) is primarily involved in the modulation of numerous morphological events in eukaryotes. It protects plants by triggering the defense system which restricts the pathogen growth. This is the first report on the isolation, purification and characterization of RGA2 from the stems of Tinospora cordifolia, a medicinal plant. The RGA2 was purified using simple two-step process using DEAE-Hi-Trap FF and Superdex 200 chromatography columns, with a high yield. The purity of RGA2 was confirmed by SDS-PAGE and identified by MALDI-TOF/MS. The purified protein was further characterized for its secondary structural elements using the far-UV circular dichroism measurements. Our purification procedure is simple two-step process with high yield which can be further used to produce RGA2 for structural and functional studies.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0400-3) contains supplementary material, which is available to authorized users.

Binding studies and biological evaluation of β-carotene as a potential inhibitor of human calcium/calmodulin-dependent protein kinase IV

“…Use of natural products in the prevention of cancer have been extensively used to reduce the side effects of chemotherapy and cancer drugs [52][53][54][55]. Since decades, attention has been given to dysregulation of CAMKIV in different diseases especially in malignancies [8,9], where it can function as an important regulator in both physiological and pathological changes.…”

In vitro Biological Properties and in Silico Studies on Tinospora Cordifolia Stem Aqueous Extract

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