Purification and characterization of Treponema hyodysenteriae hemolysin

Saheb, Samir A.; Massicotte, Luc; Picard, B

doi:10.1016/s0300-9084(80)80133-7

Cited by 33 publications

(33 citation statements)

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“…Both Saheb et al (1980) and Knoop (1 98 1) used culture supernates harvested when the maximum amount of haemolysin was present. Saheb et al (1980) found that this was at the end of the logarithmic phase whereas Knoop (198 1) obtained highest yields after incubation for only 8 h. In our study, spirochaetes were harvested when they could most actively produce haemolysin in RNA-core buffer; this occurred in the mid-tolate logarithmic phase when a very high percentage of the spirochaetes was actively motile. Ginsburg (1970) noted that for production of streptolysin S, streptococci must be metabolically active.…”

Section: Discussionmentioning

confidence: 99%

“…The unpurified haemolysin thus provided a more concentrated starting material for purification than that used by earlier workers. Moreover, since the haemolysin was produced in a simple defined buffer the initial step of ammonium sulphate precipitation used by Saheb et al (1980) and Knoop (198 1) in purifying haemolysin from culture supernates was unnecessary.…”

Section: Discussionmentioning

confidence: 99%

“…Knoop (1981) showed a 400-fold increase in the amount of haemolysin present in culture supernates if yeast ribonucleic acid core (RNA-core) was used instead of Na-RNA. The haemolysin was purified from culture supernates containing serum by Saheb et al (1980) and Knoop (1981). Saheb et al (1980) reported that the haemolysin was a high mol.…”

Section: Introductionmentioning

confidence: 99%

“…The haemolysin was purified from culture supernates containing serum by Saheb et al (1980) and Knoop (1981). Saheb et al (1980) reported that the haemolysin was a high mol. wt polypeptide or protein associated with lipids and nucleotides.…”

Section: Introductionmentioning

confidence: 99%

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Production, purification and molecular weight determination of the haemolysin of Treponema hyodysenteriae

Kent

Lemcke²,

Lysons³

1988

Journal of Medical Microbiology

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Summary. The production of haemolysin from Treponema hyodysenteriae was increased by an improved culture method and by repeated incubation of spirochaetes suspended in a buffer containing RNA-core. Ion exchange chromatography on DEAE cellulose followed by gel filtration on Sephadex GlOO yielded purified haemolysin free from extraneous protein, as judged by silver-stained polyacrylamide gels. The mol. wt of the purified haemolysin, determined by gel filtration was 19 000, a value similar to that of streptolysin S, but much lower than that previously reported.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Production, purification and molecular weight determination of the haemolysin of Treponema hyodysenteriae

Kent

Lemcke²,

Lysons³

1988

Journal of Medical Microbiology

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“…Joens et al (1982) also developed an enzyme-linked immunosorbent assay (ELISA), which uses a water soluble antigen from hot phenol and water extract of T. hyodysenteriae. Saheb et al (1980) have purified and characterized hemolysin from T. hyodysenteriae.…”

Section: Resistancementioning

confidence: 99%

Immunologic response of swine to solubilized outer envelope components of Treponema hyodysenteriae

Peterson

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Brachyspira

Stanton

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Bra.chy.spi'ra. Gr. adj. brachys short; L. fem. n. spira a coil, spiral; N.L. fem. n. Brachyspira a short spiral, describing a bacterium that resembles a short spiral. Spirochaetes / Spirochaetia / Spirochaetales / Brachyspiraceae / Brachyspira Brachyspira spirochetes are helical shaped bacteria with regular coiling patterns . Cells measure 2–11 µm by 0.2–0.4 µm. Unicellular, but dividing pairs and occasional chains of three or more cells can be observed in growing cultures. Under unfavorable growth conditions, spherical or round bodies are formed. Gram‐stain negative. Obligately anaerobic, aerotolerant . Cell ends may be blunt or pointed. Cells have a typical spirochete cell ultrastructure, consisting of an outer sheath, helical protoplasmic cylinder, and internal flagella in the space between the protoplasmic cylinder and outer sheath. Brachyspire cells have 8–30 flagella per cell depending on the species (flagellar number usually correlates with cell size and species of smaller cells have fewer flagella). Flagella attach subterminally in equal numbers at each cell end, wrap around the protoplasmic cylinder, and their free ends overlap in the middle of the cells. Flexing and creeping motility at 22°C; translational movement in liquids at 37–42°C. Cultured anaerobically on commercially available media (trypticase soy or brain heart infusion broths) containing a carbohydrate growth substrate and supplemented with defibrinated blood or animal (calf) serum. Grows at 36–42°C, optimally at 37–39°C. Population doubling times on glucose in broth cultures are 1–5 h (not reported for Brachyspira aalborgi ). Chemoorganotrophic, using various carbohydrates for growth. Possess NADH oxidase for reducing molecular oxygen. Consume oxygen during growth in culture broth beneath a 1 % oxygen atmosphere. Acetate, butyrate, H 2 , and CO 2 are major endproducts of glucose metabolism. Higher amounts of H 2 than CO 2 are produced . Weakly hemolytic except for Brachyspira hyodysenteriae which exhibits β‐hemolysis (strongly hemolytic). Associated with animal and human hosts. Some species are pathogenic. The genus Brachyspira is distinguished from other spirochete genera based on 16S rRNA gene sequences . Brachyspira species share high 16S rRNA sequence similarity with each other. Species can be differentiated by DNA–DNA relative reassociation and MLEE (multilocus enzyme electrophoresis) analyses. Similar to other spirochete genera, Brachyspira is insensitive to the antibiotic rifampin. DNA G + C content ( mol %): 24.5–27.1 ( T m ). Type species : Brachyspira aalborgi Hovind‐Hougen, Birch‐Andersen, Henrik‐Nielsen, Orholm, Pedersen, Teglbjaerg and Thaysen 1 9 8 3, 896 VP (Effective publication: Hovind‐Hougen, Birch‐Andersen, Henrik‐Nielsen, Orholm, Pedersen, Teglbjaerg and Thaysen 1982, 1135 VP .).

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Purification and characterization of Treponema hyodysenteriae hemolysin

Cited by 33 publications

References 17 publications

Production, purification and molecular weight determination of the haemolysin of Treponema hyodysenteriae

Production, purification and molecular weight determination of the haemolysin of Treponema hyodysenteriae

Immunologic response of swine to solubilized outer envelope components of Treponema hyodysenteriae

Brachyspira

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