Purification and characterization of two platelet-aggregation inhibitors, named angustatin and H-toxin TA2, from the venom of Dendroaspis angusticeps

Oyama, Etsuko; Takahashi, Hiroyuki

doi:10.1016/j.toxicon.2014.11.002

Cited by 7 publications

(2 citation statements)

References 21 publications

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“…Human envenomation by mambas can lead to neurotoxicity with no reports of local tissue damage 8 . Toxins acting on potassium channels (Kv), L-type calcium channels, acid-sensing ion channels 33 , cholinergic receptors 34 , and others acting on platelet aggregation 35 have been reported. Nonetheless, the evidence suggests that 3FTxs, such as MTs, fasciculins, and α-neurotoxins, coupled with presynaptic-acting Kunitz-type proteinase inhibitor-like (dendrotoxins) could be highly relevant for mamba venoms lethality 36,37 .…”

Section: Discussionmentioning

confidence: 99%

Horse immunization with short-chain consensus α-neurotoxin generates antibodies against broad spectrum of elapid venomous species

et al. 2019

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Antivenoms are fundamental in the therapy for snakebites. In elapid venoms, there are toxins, e.g. short-chain α-neurotoxins, which are quite abundant, highly toxic, and consequently play a major role in envenomation processes. The core problem is that such α-neurotoxins are weakly immunogenic, and many current elapid antivenoms show low reactivity towards them. We have previously developed a recombinant consensus short-chain α-neurotoxin (ScNtx) based on sequences from the most lethal elapid venoms from America, Africa, Asia, and Oceania. Here we report that an antivenom generated by immunizing horses with ScNtx can successfully neutralize the lethality of pure recombinant and native short-chain α-neurotoxins, as well as whole neurotoxic elapid venoms from diverse genera such as Micrurus , Dendroaspis , Naja , Walterinnesia , Ophiophagus and Hydrophis . These results provide a proof-of-principle for using recombinant proteins with rationally designed consensus sequences as universal immunogens for developing next-generation antivenoms with higher effectiveness and broader neutralizing capacity.

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Section: Discussionmentioning

confidence: 99%

Horse immunization with short-chain consensus α-neurotoxin generates antibodies against broad spectrum of elapid venomous species

et al. 2019

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“…6 Additionally, a metalloproteinase in Habu snake venom damages capillary endothelial cells and degrades the extracellular matrix in the vascular wall. 7,8 In a previous study, BODIPY-FL-labeled casein was used in a habu snake venom protease activity test conducted without either animals or cells. 9,10 In this method, the proteolytic cleavagemediated increase in the fluorescence quenching of BODIPY-FL-labeled casein was measured.…”

Section: Introductionmentioning

confidence: 99%

Development of a Simple Permeability Assay Method for Snake Venom-induced Vascular Damage

et al. 2018

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We have developed a novel bioassay method for the detection of snake venom based on the permeability of endothelial cell monolayers cultured in Transwell cell culture inserts. This assay relies on the proteolytic degradation of capillary basement membrane proteins, a pathophysiological event that occurs due to snakebites in vivo. Transwell permeability assays with fluorescence measurements are advantageous with regard to ethical considerations for the use of animals. The assay time was reduced from 24 h for animal tests to 2 h, and many samples could be assayed easily.

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Snake venom toxins: toxicity and medicinal applications

Chan

Cheung

Xia

et al. 2016

Appl Microbiol Biotechnol

107

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Snake venoms are complex mixtures of small molecules and peptides/proteins, and most of them display certain kinds of bioactivities. They include neurotoxic, cytotoxic, cardiotoxic, myotoxic, and many different enzymatic activities. Snake envenomation is a significant health issue as millions of snakebites are reported annually. A large number of people are injured and die due to snake venom poisoning. However, several fatal snake venom toxins have found potential uses as diagnostic tools, therapeutic agent, or drug leads. In this review, different non-enzymatically active snake venom toxins which have potential therapeutic properties such as antitumor, antimicrobial, anticoagulating, and analgesic activities will be discussed.

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Purification and characterization of two platelet-aggregation inhibitors, named angustatin and H-toxin TA2, from the venom of Dendroaspis angusticeps

Cited by 7 publications

References 21 publications

Horse immunization with short-chain consensus α-neurotoxin generates antibodies against broad spectrum of elapid venomous species

Horse immunization with short-chain consensus α-neurotoxin generates antibodies against broad spectrum of elapid venomous species

Development of a Simple Permeability Assay Method for Snake Venom-induced Vascular Damage

Snake venom toxins: toxicity and medicinal applications

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