Purification and functional analysis of the DnaK homologue from<i>Prevotella intermedia</i>OMZ 326

Kadri, Ruslina; DeVine, Deirdre; Ashraf, William

doi:10.1111/j.1574-6968.1998.tb13208.x

Cited by 8 publications

(2 citation statements)

References 22 publications

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“…Predicted virulence factors that also function as molecular chaperones, such as thioredoxin, DnaK, dps and GroEL formed a distinct network. Several years ago, the ability of P. intermedia DnaK to function as a molecular chaperone was assessed by a luciferase folding assay 34 . In periodontitis, stresses such as temperature and pH increase, increased flow of gingival crevicular fluid, and oxidative stress resulting from phagocytic cells.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of the periodontal pathogen Prevotella intermedia secretomes in biofilm and planktonic lifestyles

Karched

Bhardwaj

Qudeimat

et al. 2022

Sci Rep

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Prevotella intermedia is an important species associated with periodontitis. Despite the remarkable clinical significance, little is known about the molecular basis for its virulence. The aim of this study was to characterize the secretome of P. intermedia in biofilm and planktonic life mode. The biofilm secretome showed 109 proteins while the planktonic secretome showed 136 proteins. The biofilm and the planktonic secretomes contained 17 and 33 signal-peptide bearing proteins, 13 and 18 lipoproteins, respectively. Superoxide reductase, sensor histidine kinase, C40 family peptidase, elongation factor Tu, threonine synthase etc. were unique to biofilm. Of the ~ 30 proteins with predicted virulence potential from biofilm and planktonic secretomes, only 6 were common between the two groups, implying large differences between biofilm and planktonic modes of P. intermedia. From Gene Ontology biofilm secretome displayed a markedly higher percent proteins compared to planktonic secretome in terms of cellular amino acid metabolic process, nitrogen compound metabolic process etc. Inflammatory cytokine profile analysis revealed that only the biofilm secretome, not the planktonic one, induced important cytokines such as MIP-1α/MIP-1β, IL-1β, and IL-8. In conclusion, the revealed differences in the protein profiles of P. intermedia biofilm and planktonic secretomes may trigger further questions about molecular mechanisms how this species exerts its virulence potential in the oral cavity.

show abstract

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of the periodontal pathogen Prevotella intermedia secretomes in biofilm and planktonic lifestyles

Karched

Bhardwaj

Qudeimat

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Predicted virulence factors that also function as molecular chaperones, such as thioredoxin, DnaK, dps and GroEL formed a distinct network. Several years ago, the ability of P. intermedia DnaK to function as a molecular chaperone was assessed by a luciferase folding assay 31 . In periodontitis, stresses such as temperature and pH increase, increased ow of gingival crevicular uid, and oxidative stress resulting from phagocytic cells.…”

Section: In Ammatory Potential Of the P Intermedia Secretomesmentioning

confidence: 99%

Proteomic Analysis of the Periodontal Pathogen Prevotella Intermedia Secretomes in Biofilm and Planktonic Lifestyles

Karched

Bhardwaj

Qudeimat

et al. 2021

Preprint

View full text Add to dashboard Cite

Prevotella intermedia is an important species associated with periodontitis. Despite the remarkable clinical significance, little is known about the molecular basis for its virulence. The aim of this study was to characterize the secretome of P. intermedia in biofilm and planktonic life mode. The biofilm secretome showed 109 proteins while the planktonic secretome showed 136 proteins. The biofilm and the planktonic secretomes contained 17 and 33 signal-peptide bearing proteins, 13 and 18 lipoproteins, respectively. Superoxide reductase, sensor histidine kinase, C40 family peptidase, elongation factor Tu, threonine synthase etc. were unique to biofilm. Of the ~30 proteins with predicted virulence potential from biofilm and planktonic secretomes, only 6 were common between the two groups, implying large differences between biofilm and planktonic modes of P. intermedia. From gene ontology biofilm secretome displayed a markedly higher percent proteins compared to planktonic secretome in terms of cellular amino acid metabolic process, nitrogen compound metabolic process etc. Inflammatory cytokine profile analysis revealed that only the biofilm secretome, not the planktonic one, induced important cytokines such as MIP-1a/MIP-1b, IL-1b, and IL-8. In conclusion, the revealed differences in the protein profiles of P. intermedia biofilm and planktonic secretomes may trigger further questions about molecular mechanisms how this species exerts its virulence potential in the oral cavity.

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Immune responses to heat shock protein in Porphyromonas gingivalis‐infected periodontitis and atherosclerosis patients

Chung¹,

Kang²,

Park

et al. 2003

J of Periodontal Research

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Purification and functional analysis of the DnaK homologue fromPrevotella intermediaOMZ 326

Cited by 8 publications

References 22 publications

Proteomic analysis of the periodontal pathogen Prevotella intermedia secretomes in biofilm and planktonic lifestyles

Proteomic analysis of the periodontal pathogen Prevotella intermedia secretomes in biofilm and planktonic lifestyles

Proteomic Analysis of the Periodontal Pathogen Prevotella Intermedia Secretomes in Biofilm and Planktonic Lifestyles

Immune responses to heat shock protein in Porphyromonas gingivalis‐infected periodontitis and atherosclerosis patients

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