Purification and partial characterization of fimbriae of Vibrio cholerae O1

Ehara, Masahiko; Ishibashi, Masataka; Ichinose, Yuki; Iwanaga, Masaaki; Shimotori, Shoichi; Naito, Toshiki

doi:10.1016/0264-410x(87)90153-8

Cited by 61 publications

(60 citation statements)

References 29 publications

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“…In the previous characterization of 16K-pili (1, 3), their morphological appearance, molecular weight of the subunit protein, and immunological reactions seemed the same regardless of the strain (1,4). Therefore, within the El Tor biovar, there may be some variety of 16K-pili from the viewpoint of sugar-binding properties.…”

Section: Discussionmentioning

confidence: 88%

Pili of Vibrio cholerae Widely Distributed in Serogroup O1 Strains

Iwanaga

Nakasone

Yamashiro

et al. 1993

Microbiology and Immunology

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The distribution of Vibrio cholerae O1 pili consisting of 16 kDa subunit protein (16K-pili) was examined by Western blotting, using 211 strains from various origins and specific anti-16K-pili sera. The 16 kDa protein was detected in all 211 strains. The pili were purified from 3 El Tor and 3 classical strains, and characterized by hemagglutination and inhibition tests. All purified pili were hemagglutinative. However, the hemagglutinating activity of classical pili disappeared after exposure to 5 M urea and the agglutination induced by the classical pili was inhibited by D-mannose, alpha-methylmannoside, D-glucose and N-acetylglucosamine. On the contrary, El Tor pili were resistant to these sugars and urea.

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Section: Discussionmentioning

confidence: 88%

Pili of Vibrio cholerae Widely Distributed in Serogroup O1 Strains

Iwanaga

Nakasone

Yamashiro

et al. 1993

Microbiology and Immunology

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“…The TCG medium was used to enrich the growth of bacterial pili. TCG medium contained 0.02% thioproline, 0.3% NaHCO 3, 0.1% mono sodium l-glutamate, 1% bacto-tryptone, 0.2% yeast extract, 0.5% NaCl, 2% bacto-agar and 1 mM amino-ethyl ether-N, N, N, "n",-tetra acetic acid (EGTA) [10]. The 250-ml volume bottles were filled with 50 ml TCG media.…”

Section: Methodsmentioning

confidence: 99%

Sensitivity and Specificity of OMP Acinetobacter Baumannii Antigen Dot Blot to Detect Secretory-IgA in Urine of Patients with Acinetobacter BaumanniiInfection

Benaessa¹,

Salah²

2014

IOSRJPBS

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“…Collection pili protein S. flexneri refers to methods performed by Ehara (Ehara, et al, 1987) with modifications Sumarno (Sumarno, et al, 1991). The results of the collection of bacteria collected in a sterile bottle which was then added Tricoleracetic Acid (TCA) with a concentration of 3%.…”

Section: Protein Isolation Pili S Flexnerimentioning

confidence: 99%