Purification and Partial Characterization of a Thermostable Laccase from Pycnoporus sanguineus CS-2 with Ability to Oxidize High Redox Potential Substrates and Recalcitrant Dyes

Martínez, Sergio M. Salcedo; Gutiérrez‐Soto, Guadalupe; Garza, C.; Galván, Tania J. Villarreal; ContrerasCordero, Juan F.; Hernández-Luna, Carlos E.

doi:10.5772/56374

Cited by 5 publications

(3 citation statements)

References 42 publications

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“…Even though this is smaller than reported for this strain in another study (Salcedo-Martínez 2013), this may be due to adaptations of the techniques for the other enzymes of this work. All molecular weights reported here are relative.…”

Section: Molecular Weights Optimal Ph and Temperature Estimationcontrasting

confidence: 67%

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Selection and Characterization of a Native Pycnoporus sanguineus Strain as a Lignocellulolytic Extract Producer from Submerged Cultures of Various Agroindustrial Wastes

et al. 2015

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A native strain of Pycnoporus sanguineus (CS2) was selected because of its lignocellulolytic potential on a color-based agar plate screening system; pectinolytic and amylolytic capabilities were also assessed. The effects of different lignocellulosic substrates under submerged cultures of selected fungi on the enzymatic production of cellulases, xylanases, amylases, and laccases were evaluated. Seven different treatments, in which different combinations of wheat straw (WS), wheat bran (WB), orange peels (OP), grapefruit peels (GP), and apple peels (AP) were used, were established. Controls of 2% (w/v) carboxymethyl-cellulose (CMC) and xylan were used in a rich medium for basidiomycetes (RMB). The highest titers were achieved using OP-based cultures, with large titers of CMCases (33.5 U), avicelases (15.7 U), ß-D-glucosidase (72.9U), and xylanases (18.3 U). The best levels of amylase and laccase activity were obtained in the RMB plus CMC (RMB-CMC) (7.2 U) and in the medium OF/AP/GP (6.4 U), respectively. The relative molecular sizes of cellulase, xylanase, and amylase were 66.2, 56.5, and 90.8 kDa, respectively. Laccase and amylase had maximum activities at 60 °C whereas cellulase and hemicellulase had maximum activities at 70 °C. The optimal pH for cellulases, xylanases, and amylases was 5.0 in every case, and more than 95% activity was observed at pH 6. These results reveal some efficient operating parameters for the application of these enzymatic extracts as adjuvants to improve animal nutrition.

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Section: Molecular Weights Optimal Ph and Temperature Estimationcontrasting

confidence: 67%

“…Regarding laccase production from P. sanguineus CS2, maximum production of 7.5 U/mL in a Kirk medium modified with 350-μM cupric sulphate and 3% ethanol as inducers was reported (Salcedo-Martínez et al 2013). Under the culture conditions used in this study, laccase titers were notably lower.…”

Section: Cs2 Uamh 8156mentioning

confidence: 68%

Selection and Characterization of a Native Pycnoporus sanguineus Strain as a Lignocellulolytic Extract Producer from Submerged Cultures of Various Agroindustrial Wastes

et al. 2015

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“…From this work, the laccases from Trametes maxima CU1 (Gutiérrez-Soto et al 2011) and Pycnoporus sanguineus CS2 were purified, and their ability to degrade dyes with high demand in the region were characterized (Salcedo-Martínez et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Native Macrofungi that Produce Lignin-Modifying Enzymes, Cellulases, and Xylanases with Potential Biotechnological Applications

et al. 2015

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With the aim of identifying and exploiting the mycological resources available in the Mexican Sierra Madre Oriental, the lignocellulolytic and pectinolytic potential of autochthonous fungi were evaluated in the present work. A solid media selection system was established in which 74 isolated strains were tested and compared to six international reference strains. The macrofungi Xylaria sp CS121, Inonotus sp CU7, Basidiomycete CH32, Basidiomycete CH23, Xylaria poitei, and Trametes maxima CU1 showed the highest cellulolytic and pectinolytic potential. The greatest lignolytic capability was exhibited by T. maxima CU1 and Pycnoporus sanguineus CS43. Under stirred submerged culture, T. maxima CU1 (cellulases, cellobiose dehydrogenase, manganese peroxidase (MnP), and laccase, with 200, 359, 51, and 267 U/L, respectively) and Xylaria sp CS121 (198 U/L of xylanases) were the highest enzymatic producers. Under stationary conditions, the best producers were Inonotus sp CU7 for cellulases, P. sanguineus CS43 for cellobiose dehydrogenase and laccase, and T. maxima CU1 for xylanases and MnP (242, 467, 35, 165, and 31 U/L, respectively). These results demonstrate the efficiency of enzymatic profiling as a tool for enzyme discovery with Mexican native fungi.

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Combinatorial decolorization performance of Pycnoporus sanguineus MUCL 38531 sourced recombinant laccase/mediator systems on toxic textile dyes

Malcı

Gur

Tamerler

et al. 2022

Int. J. Environ. Sci. Technol.

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Purification and Partial Characterization of a Thermostable Laccase from Pycnoporus sanguineus CS-2 with Ability to Oxidize High Redox Potential Substrates and Recalcitrant Dyes

Cited by 5 publications

References 42 publications

Selection and Characterization of a Native Pycnoporus sanguineus Strain as a Lignocellulolytic Extract Producer from Submerged Cultures of Various Agroindustrial Wastes

Selection and Characterization of a Native Pycnoporus sanguineus Strain as a Lignocellulolytic Extract Producer from Submerged Cultures of Various Agroindustrial Wastes

Native Macrofungi that Produce Lignin-Modifying Enzymes, Cellulases, and Xylanases with Potential Biotechnological Applications

Combinatorial decolorization performance of Pycnoporus sanguineus MUCL 38531 sourced recombinant laccase/mediator systems on toxic textile dyes

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