Purification and Partial Characterization of a Hemagglutinin From Seeds of Jatropha Curcas

Abstract

An extract from Jatropha curcas seeds, purified by gel filtration on Sephadex G‐75 and Sephacryl S‐200, yielded an active hemagglutinin of high purity as assessed by electrophoresis and isoelectric focussing. The hemagglutinin had a molecular weight of around 660,000 and a pI value of 5.75. The molecule was composed of two different subunits of molecular weights 23,450 and 11,500. Amino acid analysis suggested that the molecule lacked 1/2 cystine but contained a high proportion of acidic and basic amino acids. Agglutination of trypsinized erythrocytes, groups A, B and O, took place over the range pH 4–10, and was prevented by D‐galactose, D‐galactosamine and N‐acetyl‐D‐galactosamine. The hemagglutinin has only a weak binding capacity for D‐galactose. Its activity was stable up to 60°C; at 80°C activity was lost in 50 min.

Biochemical evaluation of protein fractions from physic nut (<i>Jatropha curcas</i> L.)

“…3B). Purified hemagglutinin from J. curcas seeds had a pI value of 5.75 (Cano-Asseleih et al, 1989), while in curcin, the pI value was 8.54 (Juan Lin et al, 2010).…”

Assessment of lectin activity in a toxic and a non-toxic variety ofJatropha curcas using latex agglutination and haemagglutination methods and inactivation of lectin by heat treatments

“…The plant is widely distributed in wild or semicultivated stands in Central and South America, Africa, India and South East Asia (Cano 1986 ;Cano et al 1989 ;Heller 1996). The seed weighs about 0É75 g and the kernel represents about 65% of the seed mass.…”

Phytotranslocation of Fe by biodiesel plant Jatropha curcas L. grown on iron rich wasteland soil

“…The seeds contain 27-40% oil that can be processed to produce a high-quality biodiesel fuel. Genus Jatropha with 172 species is native to Central America and is also widely distributed in Africa and Asia (Cano-Asseleih et al, 1989;Mangkoedihardjo and Sunahmadia, 2008). Among the various Jatropha species, J. curcas is one which is widely distributed in India (Fairless, 2007).…”