Purification and Properties of Inactive Liver Phosphorylase^*

Abstract

The material presented is taken in part from the thesis submitted in 1962 to the Graduate Faculty of the University of Washington in partial fulfillment of the requirements for the Ph.D. degree.

Molecular Studies on Glycogen Storage Diseases

“…Rabbit muscle phosphorylase was prepared according to Fisch er and Krebs [11] and recrystallized four times. Rabbit liver phosphorlyase was purified according to Appleman et al [2]. Rabbit muscle amylo-1,6-glucosidase was purified according to Brown and Brown [4] up to the DEAE chromatography stage.…”

The control of glycogen metabolism in the liver

“…The liver phosphophosphorylase (phosphorylase a) is the active form of the enzyme; its activity is increased by 15 to 40% upon the addition of 10 -3 M AMP [3,4]. The dephosphoenzyme (phosphorylase b) is completely inactive in the absence of AMP; its activity in the presence of the nucleotide is nearly zero in pig or rabbit liver [5], but reaches 15% of phosphorylase a in dog liver [3] and 25% in mouse liver [6].…”

Phosphorylase: A New Isozyme in Rat Hepatic Tumors and Fetal Liver

“…Major structural similarities of the 24 NOVEMBER 1972 australopithecine pelvis to that in modern man are convincing evidence of advanced adaptation to bipedality (8). However, certain obvious dissimilarities, such as the greater flaring of the iliac blades, may be explicable in terms of a more abducted femoral excursion.…”

“…All three phosphorylases are predominantly in the b form, as indicated by their low activity without adenosine monophosphate (AMP). The tumor form is further distinguished from the liver form in that it does not require sulfate ion for activation (8). Other poorly differentiated, rapid-growing hepatomas such as the Morris 3924A hepatoma give a pattern similar to that of the Novikoff hepatoma, whereas the well-differentiated, slow-growing Morris hepatoma 20 possesses both the tumor form as a minor, and the liver An antiserum was prepared as a rabbit serum globulin fraction after serial injections of a crystalline preparation of rat skeletal muscle phosphorylase prepared as described by Sevilla and Fischer (14), together with complete Freund's adjuvant.…”