Purification, biochemical characterization and self-assembled structure of a fengycin-like antifungal peptide from Bacillus thuringiensis strain SM1

Abstract

An antifungal lipopeptide fengycin, producing strain SM1 was isolated from farm land soil sample and identified as Bacillus thuringiensis strain SM1 by using 16S rDNA analysis. Fengycin detected in the culture extract was further purified using HPLC and showed a molecular mass of 1492.8 Da by MALDI-TOF-MS analysis. Purified fengycin was allowed to construct their self-assembled structure onto a hydrophobic surface showing a clear improvement of antibacterial activity. In self-assembly, fengycin adapts a spherical micelle core shell like structure. Self-assembled fengycin may be a successful antimicrobial compound modifying its action from confined antifungal function. Besides it can open up a new area of research in supramolecular lipopeptide based compound making. This can revealed the mode of action of this unique self-assembled structure to fully evaluate its potential for use as an antimicrobial drug to control the emergence of bacterial infection.

<i>Bacillus subtilis Strains</i> with Antifungal Activity against the Phytopathogenic Fungi

“…Fengycin is known to affect the biological membranes of fungi, causing membrane pore formation, however, the precise mechanism of fengycin's action and the intrinsic biochemical determinants implicated in its antifungal activity have not been fully elucidated [31]. Simultaneous production of fengycins and surfactins could be important for the efficiency of F. graminearum control by strain B. subtilis SG6 [26].…”

Structural insights into Cn‐AMP1, a short disulfide‐free multifunctional peptide from green coconut water

“…It is observed that the integral values of NOE i À i + 3 and NH-NH are in the same order of magnitude, suggesting close spatial distances (Table S3). On the other hand, the interactions i À i + 3 and NH-NH for 5HN-2HA and 9HN-8HN, show clear differences due to structural flexibility caused by 4 Gly and 8 Gly, respectively.…”

“…Furthermore, in addition to multiple functions, there are also a wide number of AMP sources including microorganisms, invertebrates, animals and plants [3][4][5]. In the case of plant tissues, AMPs have also been isolated from flowers, leaves, roots and seeds, among others [5][6][7][8].…”

Antifungal Screening of Bioprotective Isolates against Botrytis cinerea, Fusarium pallidoroseum and Fusarium moniliforme

“…B. megaterium, B. coagulans, B. thuringiensis BT2 and several B. amyloliquefaciens isolates demonstrated both strong biosurfactant activity and suppression of B. cinerea, F. pallidoroseum, and F. moniliforme, implying the ability to synthesize antifungal lipopeptides. The production of antifungal lipopeptides has been identified in other strains of the species of Bacillus used in this study, including B. amyloliquefaciens [11,12,24], B. coagulans [21], B. megaterium [23], and B. thuringiensis [15,16,57]. B. pumilus and O. sojae demonstrated weak biosurfactant activity but no fungal suppression.…”