Purification, characterization, and anticancer and antioxidant activities of l-glutaminase from Aspergillus versicolor Faesay4

Awad, Mohamed F.; El-Shenawy, Fareed Shawky; El-Gendy, Mervat Morsy Abbas Ahmed; El-Bondkly, Esraa Ahmed Mohamed

doi:10.1007/s10123-020-00156-8

Cited by 19 publications

(9 citation statements)

References 29 publications

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“…9 Many terrestrial fungi, including Penicillium, Phoma, Aspergillus, Phomopsis, Trichoderma, Cladosporium, Drechslera, Mucor, Chaetomium, and Emericella species, have been shown to produce L-glutaminase. [10][11][12][13][14][15][16] Apart from terrestrial fungus, marine fungus are also used to produce L-glutaminase. There are relatively few reports available on L-glutaminase production from marine fungi.…”

Section: Discussionmentioning

confidence: 99%

“…L-glutaminase formation increased significantly on the 6th day and 7 th day of fermentation and then gradually decreased production course in Aspergillus versicolor Faesay4 and M. circinelloides due to enzyme degradation. 10 Five different carbon sources such as glucose, fructose, lactose, starch and maltose were studied for L-glutaminase production. Fusarium solani-melongenea strain CRI 24 produced highest enzyme activity with starch (0.2%) as substrate; maltose and lactose supplementation decreased enzyme activity.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, substrate such as sucrose and dextrose were found to be good carbon source for L-glutaminase production by A. versicolor Faesay4, F. oxysporum and T. longibrachiatum. 10,26,27 It has been reported that fungi show remarkable selectivity for nitrogen source that is present in the medium. The production of enzymes is significantly impacted by the availability of nitrogen.…”

Section: Discussionmentioning

confidence: 99%

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Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation

Vineetha,

Aldabaan,

et al. 2024

J. Pure Appl. Microbiol.

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L-glutaminase is a unique enzyme with catalytic activity and the ability to modulate glutamine levels, making it a valuable enzyme with numerous potential applications. L-glutaminase triggers a distinctive reaction by converting L-glutamine into glutamic acid while releasing ammonia concurrently. This enzymatic process holds potential applications across diverse industries, notably in food and pharmaceuticals. The primary objective of the present research was to identify and isolate a fungal strain proficient in L-glutaminase production from soil found in maritime environments. The physical and nutritional conditions were optimized for maximum synthesis of L-glutaminase under solid state fermentation (SSF) and submerged fermentation conditions (SmF). The isolated organism was identified as Fusarium solani-melongenae strain CRI 24 by morphological and 18S rRNA analysis. The optimum carbon source under SmF and SSF was found to be starch (0.2% w/v). Wheat bran as solid substrate among others showed optimum enzyme activity. On the seventh day of incubation, at pH 8.0 and 0.7% L-glutamine concentration under SSF and SmF, the highest enzyme activity was detected. The greatest enzyme activity in SSF was seen at a moisture content of 10%. Fusarium solani-melongenae species produced the enzyme under optimal conditions and 4.20 and 4.73-fold increase (from 0.8 U/mL to 3.61 U/mL and from 0.781 U/mL to 3.69 U/mL) was achieved after optimization in submerged and in solid state fermentation, respectively. The selective isolation and optimization processes described in this work are a promising technique for the industrial production of L-glutaminase and can be applied in the pharmaceutical and food industries.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation

Vineetha,

Aldabaan,

et al. 2024

J. Pure Appl. Microbiol.

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“…Matrix metalloproteinase catalyzes the breakdown of the peptide bond in the clot formation molecule by adding a water molecule. These proteins showed the expression against tumor cells [217].…”

Section: Medical and Pharmaceutical Industrymentioning

confidence: 99%

Fungal Proteases as Emerging Biocatalysts to Meet the Current Challenges and Recent Developments in Biomedical Therapies: An Updated Review

Naeem

Manzoor²,

Abid

et al. 2022

JoF

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With the increasing world population, demand for industrialization has also increased to fulfill humans’ living standards. Fungi are considered a source of essential constituents to produce the biocatalytic enzymes, including amylases, proteases, lipases, and cellulases that contain broad-spectrum industrial and emerging applications. The present review discussed the origin, nature, mechanism of action, emerging aspects of genetic engineering for designing novel proteases, genome editing of fungal strains through CRISPR technology, present challenges and future recommendations of fungal proteases. The emerging evidence revealed that fungal proteases show a protective role to many environmental exposures and discovered that an imbalance of protease inhibitors and proteases in the epithelial barriers leads to the protection of chronic eosinophilic airway inflammation. Moreover, mitoproteases recently were found to execute intense proteolytic processes that are crucial for mitochondrial integrity and homeostasis function, including mitochondrial biogenesis, protein synthesis, and apoptosis. The emerging evidence revealed that CRISPR/Cas9 technology had been successfully developed in various filamentous fungi and higher fungi for editing of specific genes. In addition to medical importance, fungal proteases are extensively used in different industries such as foods to prepare butter, fruits, juices, and cheese, and to increase their shelf life. It is concluded that hydrolysis of proteins in industries is one of the most significant applications of fungal enzymes that led to massive usage of proteomics.

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“…Several secondary metabolites of A . versicolor serve as a source for drug discovery [13–15]. Various secondary metabolites, such as anthraquinone, alkaloids, semi terpenoids, diketone piperazine, and diphenyl ether have been isolated from A .…”

Section: Introductionmentioning

confidence: 99%

Analysis ofAspergillus versicolorexudate composition

2022

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Aspergillus versicolor, a widely distributed fungus, is associated with pollution and carcinogenic hazards. This study aimed to examine the functions of the A. versicolor exudate and laid a scientific foundation for improving our understanding, utilization, and control of A. versicolor. The A. versicolor exudate proteome, ion content, and amino acid components were determined using label-free quantitation, atomic absorption spectrophotometry, and highperformance liquid chromatography, respectively. In total, 502 proteins were identified in the A. versicolor exudate. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and cluster of orthologous group analyses were used to annotate the functional classification and pathways of the aligned proteins.Proteins identified in the exudate were mainly enriched in carbohydrate metabolic process, translation, oxidoreductase activity, oxidoreductase activity, hydrolase activity, cell wall-related processes, catalytic activity, and unknown functions. The exudate comprised Na, K, Ca, Fe, and Mg cations. Among the 17 types of amino acids detected in the exudate, 7 were essential and 10 were nonessential. The exudate may be involved in the vital processes of A. versicolor. Additionally, the exudate may play an important role in the growth, development, reproduction, homeostasis, nutrient supply for regrowth, and virulence of A. versicolor.

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Purification, characterization, and anticancer and antioxidant activities of l-glutaminase from Aspergillus versicolor Faesay4

Cited by 19 publications

References 29 publications

Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation

Production and Optimization of L-glutaminase from Halophilic Fusarium solani-melongenae Strain CRI 24 under Submerged and Solid State Fermentation

Fungal Proteases as Emerging Biocatalysts to Meet the Current Challenges and Recent Developments in Biomedical Therapies: An Updated Review

Analysis ofAspergillus versicolorexudate composition

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