Purification, characterization and anticoagulant activity of the polysaccharides from green tea

Cai, Wei; Xie, Li; Chen, Yong; Zhang, Hong

doi:10.1016/j.carbpol.2012.10.057

Cited by 124 publications

(65 citation statements)

References 19 publications

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“…3). It was observed that all samples exhibited typical major broad stretching peak appeared around 3,600-3,200 cm -1 for the -OH group (Cai et al 2013). The relative stronger absorption peak at 1,650.74 cm -1 for N-H bending vibration might be related to the little content of protein in EPS-1 (Gan et al 2011).…”

Section: Uv and Ft-ir Spectra Of Eps-1 And Eps-2mentioning

confidence: 88%

Purification, characterization, antioxidant activity and anti-aging of exopolysaccharides by Flammulina velutipes SF-06

Cui

Gao²,

Zhang

et al. 2014

Antonie van Leeuwenhoek

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The main objective of this work was to purify the exopolysaccharides (EPS) of Flammulina velutipes SF-06 and investigate the relationship between the different purified fractions and bioactive activity. Two fractions (EPS-1 and EPS-2) were separated and purified by DEAE-52 cellulose and Sephadex G-100 cellulose column chromatography. Monosaccharides composition analysis by gas chromatography indicated that EPS, EPS-1 and EPS-2 were heteropolysaccharides in which rhamnose was a major component. Fourier transform infrared (FT-IR) analysis detected furanose-ring in EPS-1 and EPS-2. All fractions possessed considerable antioxidant activity, while EPS-2 has stronger antioxidant activity than EPS and EPS-1 in vitro. The EPS also exhibited potent anti-aging activation in mice, such as increased catalase and total antioxidant capacity, and decreasing the malondialdehyde (MDA) content. Both the antioxidant in vitro and anti-aging in vivo potentials of EPS could be further utilized in the food industry.

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Section: Uv and Ft-ir Spectra Of Eps-1 And Eps-2mentioning

confidence: 88%

Purification, characterization, antioxidant activity and anti-aging of exopolysaccharides by Flammulina velutipes SF-06

Cui

Gao²,

Zhang

et al. 2014

Antonie van Leeuwenhoek

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“…The peaks toward 1000-1200 cm −1 on the two spectra suggested the presence of C─O─C and C─O─H link bonds, indicating the presence of pyranose. Finally, the peak that appeared at 874 cm −1 of THWP-1 was attributed to D-Man in pyranose [49]. A characteristic band approximately at 816 cm −1 in purified THWP was due to α-D-Glc [49].…”

Section: Ft-ir Spectra Of Thwp Fractionsmentioning

confidence: 94%

Extraction, characterization and antioxidant activity of water-soluble polysaccharides from Tuber huidongense

Chen

Zhang

Chunxia

et al. 2016

International Journal of Biological Macromolecules

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“…The interpretations of the 1 H/ 1 H correlated spectroscopy (COSY), 1 H/ 13 C heteronuclear single-quantum coherence (HSQC), and heteronuclear multiple bond coherence (HMBC) spectra were recorded using a state-time proportion phase incrementation for quadrature detection in the indirect dimension. 13 C spectrum was determined with acetone as the internal standard (methyl C atomic chemical shift of 31.5 ppm), and the 1 H spectrum was done with the HOD peak as calibration standard (δ 4.85 ppm) [27,28].…”

Section: Nuclear Magnetism Spectra Analysismentioning

confidence: 99%

“…H-1-2 was labeled with sodium pertechnetate ( 99m Tc) using a stannous chloride (SnCl 2 ) method [31]. DMSO solution H-1-2 (10 mg/mL pH 6.0) was mixed with 300 mL of NaCl aqueous 99m Tc (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) in the presence of 20 mL of HCl aqueous SnCl 2 (2 mg/mL) reaction, at 50 • C for 60 min. The labeling efficiency of 99 mTc to CS was assessed using thin layer chromatography (TLC), using silica gel coated TLC plates (Ann Arbor, MI, USA).…”

Section: Animal Study-biodistribution Of H-1-2mentioning

confidence: 99%

Structural Elucidation of a Novel Polysaccharide from Pseudostellaria heterophylla and Stimulating Glucose Uptake in Cells and Distributing in Rats by Oral

et al. 2016

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Abstract:The semi-refined polysaccharide of Pseudostellaria heterophylla is a complex polysaccharide that exhibits significantly hypoglycemic activities. A novel homogeneous polysaccharide, named as H-1-2, was isolated from the semi-refined polysaccharide. The mean molecular weight of H-1-2 was 1.4 × 10 4 Da and it was only composed of D-glucose monosaccharide. Structure elucidation indicated that H-1-2 contains pyranride, and has the characteristics of the α-iso-head configuration, a non-reducing end (T-), 4-, 1,6-, and 1,4,6-connection, in all four ways to connect glucose. H-1-2 was a type of glucan, where chemical combination exists in the main chain between 1→4 linked glucose, and contains a small amount of 1,6-linked glucose, which was in the branched chain. In vitro HepG2, 3T3-L1, and L6 cells were used to assess cellular glucose consumption and cellular glucose uptake by glucose oxidase, and the transport of 2-NBDG fluorescence probe results showed that H-1-2 could clearly increase glucose uptake and utilization in muscle and adipose cells, which is beneficial to screen for in the discovery of anti-diabetes lead compounds. H-1-2 was labeled with radioisotopes ( 99m Tc-pertechnetate). 99m Tc-labeled-H-1-2 was performed by SPECT/CT analysis images after oral administration in rats. At 4 h post ingestion, about 50% of the radioactivity was observed in the intestine. No significant radioactivity was found in the heart, liver, and kidney, conjecturing that absorption of 99m Tc-labeled H-1-2 might, via intestinal mucosa, be absorbed into systemic circulation. This problem, as to whether the polysaccharide is absorbed orally, will need further examination.

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Purification, characterization and anticoagulant activity of the polysaccharides from green tea

Cited by 124 publications

References 19 publications

Purification, characterization, antioxidant activity and anti-aging of exopolysaccharides by Flammulina velutipes SF-06

Purification, characterization, antioxidant activity and anti-aging of exopolysaccharides by Flammulina velutipes SF-06

Extraction, characterization and antioxidant activity of water-soluble polysaccharides from Tuber huidongense

Structural Elucidation of a Novel Polysaccharide from Pseudostellaria heterophylla and Stimulating Glucose Uptake in Cells and Distributing in Rats by Oral

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