Purification, characterization and end product analysis of dextran degrading endodextranase from Bacillus licheniformis KIBGE-IB25

Zohra, Rashida Rahmat; Aman, Afsheen; Ansari, Asma; Haider, Muhammad Samee; Qader, Shah Ali Ul

doi:10.1016/j.ijbiomac.2015.04.007

Cited by 34 publications

(22 citation statements)

References 45 publications

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“…Endo-type Cadex showed high specificity towards dextrans containing α-1,6 glucosidic bonds. Moreover, the main hydrolysis products of Cadex were isomaltooligomers [ 30 , 32 , 33 ]. Dextranase from Chaetomium [ 34 ], Aspergillus [ 35 ], Penicillium [ 36 ], and Fusarium [ 37 ] synthesize comparatively low amounts of glucose and higher amounts of isomaltooligosaccharides.…”

Section: Discussionmentioning

confidence: 99%

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Purification and Characterization of a Biofilm-Degradable Dextranase from a Marine Bacterium

et al. 2018

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This study evaluated the ability of a dextranase from a marine bacterium Catenovulum sp. (Cadex) to impede formation of Streptococcus mutans biofilms, a primary pathogen of dental caries, one of the most common human infectious diseases. Cadex was purified 29.6-fold and had a specific activity of 2309 U/mg protein and molecular weight of 75 kDa. Cadex showed maximum activity at pH 8.0 and 40 °C and was stable at temperatures under 30 °C and at pH ranging from 5.0 to 11.0. A metal ion and chemical dependency study showed that Mn2+ and Sr2+ exerted positive effects on Cadex, whereas Cu2+, Fe3+, Zn2+, Cd2+, Ni2+, and Co2+ functioned as inhibitors. Several teeth rinsing product reagents, including carboxybenzene, ethanol, sodium fluoride, and xylitol were found to have no effects on Cadex activity. A substrate specificity study showed that Cadex specifically cleaved the α-1,6 glycosidic bond. Thin layer chromatogram and high-performance liquid chromatography indicated that the main hydrolysis products were isomaltoogligosaccharides. Crystal violet staining and scanning electron microscopy showed that Cadex impeded the formation of S. mutans biofilm to some extent. In conclusion, Cadex from a marine bacterium was shown to be an alkaline and cold-adapted endo-type dextranase suitable for development of a novel marine agent for the treatment of dental caries.

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Section: Discussionmentioning

confidence: 99%

“…Isomaltooligosaccharides can promote the growth and proliferation of Bifidobacteria and Lactobacillus [ 1 , 38 ]. Numerous isomaltooligosaccharides are prebiotics, which are produced endodextranases and have garnered much commercial interest [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Purification and Characterization of a Biofilm-Degradable Dextranase from a Marine Bacterium

et al. 2018

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“…mutant with the optimal temperature of 60 °C. Rashida et al [11] purified a dextranase from Bacillus licheniformis KIBGE-IB25, of which the specific activity was 1405 U/mg. Compared with dextranases from bacteria, those from fungi may achieve higher activities.…”

Section: Introductionmentioning

confidence: 99%

Purification, Characterization and Degradation Performance of a Novel Dextranase from Penicillium cyclopium CICC-4022

RuiJie

Zhong

Xie

et al. 2019

IJMS

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A novel dextranase was purified from Penicillium cyclopium CICC-4022 by ammonium sulfate fractional precipitation and gel filtration chromatography. The effects of temperature, pH and some metal ions and chemicals on dextranase activity were investigated. Subsequently, the dextranase was used to produce dextran with specific molecular mass. Weight-average molecular mass (Mw) and the ratio of weight-average molecular mass/number-average molecular mass, or polydispersity index (Mw/Mn), of dextran were measured by multiple-angle laser light scattering (MALS) combined with gel permeation chromatography (GPC). The dextranase was purified to 16.09-fold concentration; the recovery rate was 29.17%; and the specific activity reached 350.29 U/mg. Mw of the dextranase was 66 kDa, which is similar to dextranase obtained from other Penicillium species reported previously. The highest activity was observed at 55 °C and a pH of 5.0. This dextranase was identified as an endodextranase, which specifically degraded the α-1,6 glucosidic bonds of dextran. According to metal ion dependency tests, Li+, Na+ and Fe2+ were observed to effectively improve the enzymatic activity. In particular, Li+ could improve the activity to 116.28%. Furthermore, the dextranase was efficient at degrading dextran and the degradation rate can be well controlled by the dextranase activity, substrate concentration and reaction time. Thus, our results demonstrate the high potential of this dextranase from Penicillium cyclopium CICC-4022 as an efficient enzyme to produce specific clinical dextrans.

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“…The freeze-dried crude EPS was redissolved in deionized water and applied to a Sephacryl S-200 gel filtration column (2.0 cm × 60 cm) for further purification [ 46 ]. The sample was detected by UV wavelength detector and elution with deionized water at a flow rate of 0.6 mL/min.…”

Section: Methodsmentioning

confidence: 99%

Optimization of EPS Production and Characterization by a Halophilic Bacterium, Kocuria rosea ZJUQH from Chaka Salt Lake with Response Surface Methodology

Jiao²,

et al. 2017

Molecules

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With the rising awareness of microbial exopolysaccharides (EPSs) application in various fields, halophilic microorganisms which produce EPSs have received broad attention. A newly identified Kocuria rosea ZJUQH CCTCC M2016754 was determined to be a moderate halobacterium on account of its successful adaption to the environment containing 10% NaCl. The optimal combination of fermentation medium compositions on EPS production was studied. In this work, a fractional factorial design was adopted to investigate the significant factors that affected EPS production. The factors of KCl and MgSO4 were found to have a profound impact on EPS production. We utilized central composite design and response surface methodology to derive a statistical model for optimizing the submerged culture medium composition. Judging from these experimental results, the optimum culture medium for producing EPSs was composed of 0.50% casein hydrolysate, 1.00% sodium citrate, 0.30% yeast extract, 0.50% KCl, 0.50% peptone, and 5.80% MgSO4 (initial pH 7.0). The maximal EPS was 48.01 g/L, which is close to the predicted value (50.39 g/L). In the validation experiment, the highest concentration of 70.64 g/L EPSs was obtained after 120 h under the optimized culture medium in a 5-L bioreactor. EPS from this bacterium was also characterized by differential scanning calorimetry (DSC) and Fourier transform infrared analysis (FT-IR). The findings in this study imply that Kocuria rosea ZJUQH has great potential to be exploited as a source of EPSs utilized in food, the pharmaceutical and agriculture industry, and in the biotreatment of hypersaline environments.

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Purification, characterization and end product analysis of dextran degrading endodextranase from Bacillus licheniformis KIBGE-IB25

Cited by 34 publications

References 45 publications

Purification and Characterization of a Biofilm-Degradable Dextranase from a Marine Bacterium

Purification and Characterization of a Biofilm-Degradable Dextranase from a Marine Bacterium

Purification, Characterization and Degradation Performance of a Novel Dextranase from Penicillium cyclopium CICC-4022

Optimization of EPS Production and Characterization by a Halophilic Bacterium, Kocuria rosea ZJUQH from Chaka Salt Lake with Response Surface Methodology

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