Purification, characterization, and gene cloning of an Aspergillus fumigatus polyhydroxybutyrate depolymerase used for degradation of polyhydroxybutyrate, polyethylene succinate, and polybutylene succinate

Jung, Hsin-Wei; Yang, Ming‐Der; Su, Ruey-Chih

doi:10.1016/j.polymdegradstab.2018.06.002

Cited by 50 publications

(17 citation statements)

References 39 publications

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“…A tick indicates clear evidence for microbial degradation of the polymer, according to our analyses of the contemporary literature (see supplementary material). The category 'other' contains several plastic polymers, including the biopolymers polyhydroxyalkanoate (PHA; Tokiwa and Calabia (2004)) and polybutylene succinate (PBS; Jung et al (2018)) and fossil fuel-based polymers such as poly(butylene adipate-co-terephthalate) (PBAT; Muroi et al (2017)). They are each biodegradable but only produced in relatively minor quantities globally and so are not highlighted individually in this plot (i.e.…”

Section: Introductionmentioning

confidence: 99%

Microbial abilities to degrade global environmental plastic polymer waste are overstated

Lear

Maday

Gambarini

et al. 2022

Environ. Res. Lett.

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Internationally, the environmental damage caused by the improper disposal of approximately 100 Mt of plastic waste per annum is of growing concern. Attempts to address this issue have generated many hundreds of scientific studies announcing the discovery of novel plastic-degrading microorganisms and their respective enzymes. On closer inspection, however, evidence remains sparse for the microbial degradation of most of the plastic polymers produced globally. We systematically surveyed the international literature to confirm how many microorganisms proposed to degrade plastics (n = 664) cause substantial (i.e.,  20% mass) losses of virgin polymer, rather than losses of plastic additives, filler, and/or shedding of polymer micro-fragments. Additionally, we noted where degradation was only demonstrated for artificially aged polymer since physicochemical ageing procedures increase the abundance of monomers and oligomers such that they may be degraded by microbial activity. Additionally, artificial ageing may introduce functional groups to the polymer backbone, creating more locations susceptible to microbial degradation than would otherwise occur in the environment. We identified multiple studies demonstrating the effective microbial degradation of heterochain plastic polymers such as polylactic acid, polycaprolactone and polyethylene terephthalate (i.e., polymers containing elements other than carbon in the backbone structure). However, in the literature, we find no evidence for the substantial degradation of unadulterated polyethylene, polypropylene, polystyrene or polyvinyl chloride, homochain polymers which represent the overwhelming majority of global plastics production. Current research demonstrates that the pre-treatment of plastics with elevated temperature or UV-light may speed physicochemical plastic degradation, with valuable applications for downstream microbial processing. However, evidence for the microbial degradation of most plastic polymers in current circulation is lacking. We outline simple criteria that should be met before announcing the microbial degradation of plastic polymers. We hope this may help to address largely unsubstantiated expectations that microorganisms can degrade many plastic polymers in situ.

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Section: Introductionmentioning

confidence: 99%

Microbial abilities to degrade global environmental plastic polymer waste are overstated

Lear

Maday

Gambarini

et al. 2022

Environ. Res. Lett.

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“…The goal of our work was to study and determine the adsorption kinetics of a hydrolase enzyme catalyzing the decomposition of PHB [25][26][27][28] independently of the chemical reaction itself. In order to achieve this goal, we used the method described above, i.e.…”

Section: Introductionmentioning

confidence: 99%

The role of enzyme adsorption in the enzymatic degradation of an aliphatic polyester

Polyák

Urban

Nagy

et al. 2019

Enzyme and Microbial Technology

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The enzyme catalyzed degradation of poly(3-hydroxybutyrate) (PHB) is a two-step process consisting of the adsorption of the enzyme on the surface of a PHB substrate and the cleavage of ester bonds. A deactivated enzyme was prepared by point mutagenesis to separate the two steps from each other. Measurements carried out with active and inactive enzymes on PHB particles proved that mutagenesis was successful and the modified enzyme did not catalyze degradation. Based on the Michaelis-Menten approach a kinetic model was proposed which could describe the processes quantitatively, the agreement between prediction and the measured data was excellent. The separation of the two processes allowed the determination of the adsorption kinetics of the enzyme; the rate constants of the adsorption and desorption process were determined for the first time. Comparison of these constants to reaction rates showed that adsorption is not instantaneous and can be the ratedetermining step. The area occupied by an enzyme molecule was also determined (13.1 nm 2) and it was found to be smaller than the value published in the literature (178 nm 2). The separation of the two steps makes possible the prediction and control of the degradation process.

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“…Our results demonstrate that recombinant MRCUT1 can efficiently hydrolyze biodegradable polyesters like PES and PCL; however, it can also degrade long-persistence polyesters like PET in residues of water storage bottles in 21 days. After 6 days, PES films can be almost totally degraded (95%) by recombinant F. solani cutinase but cannot be degraded by polyhydroxybutyrate depolymerase from Aspergillus fumigatus ( 23 , 37 ). MRCUT1 cutinase can degrade 43% after 21 days.…”

Section: Discussionmentioning

confidence: 99%

Expression of a Cutinase of Moniliophthora roreri with Polyester and PET-Plastic Residues Degradation Activity

et al. 2021

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Purification, characterization, and gene cloning of an Aspergillus fumigatus polyhydroxybutyrate depolymerase used for degradation of polyhydroxybutyrate, polyethylene succinate, and polybutylene succinate

Cited by 50 publications

References 39 publications

Microbial abilities to degrade global environmental plastic polymer waste are overstated

Microbial abilities to degrade global environmental plastic polymer waste are overstated

The role of enzyme adsorption in the enzymatic degradation of an aliphatic polyester

Expression of a Cutinase of Moniliophthora roreri with Polyester and PET-Plastic Residues Degradation Activity

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