Purification, characterization and immunoregulatory activity of a polysaccharide isolated from <i>Hibiscus sabdariffa</i> L.

Zheng, Donghai; Zou, Ye; Cobbina, Samuel Jerry; Wang, Wei; Li, Qian; Chen, Yao; Feng, Weiwei; Zou, Yanmin; Zhao, Ting; Zhang, Min; Yang, Liuqing

doi:10.1002/jsfa.7908

Cited by 20 publications

(11 citation statements)

References 52 publications

(54 reference statements)

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“…They are important components involved in many physiological functions in all living organisms. Recently, more and more experiments have indicated that polysaccharides possessed a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant, anti‐inflammatory, and immunomodulatory activity and so on (Song, Li, Hu, Ni, & Li, ; Wang et al, ; Zheng et al, ). In addition, the polysaccharides were widely used in functional food and pharmaceutical industries for no toxic side effects (Xie et al, ).…”

Section: Introductionmentioning

confidence: 99%

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Xiang

Лі

et al. 2019

J Food Biochem

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A neutral polysaccharide (DIP‐1) from Duchesnea indica (Andr.) Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE‐52 cellulose anion‐exchange column and Sephadex G‐100 gel column). The physicochemical properties of DIP‐1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV–visible spectrophotometry, scanning electron microscope and Congo red test. The results indicated that DIP‐1 was consisted of mannose, glucosamine, glucose, galactose and arabinose in a ratio of 1.00:0.42:18.36:14.17:0.81, and its molecular weight was 218.3 kDa. Meanwhile, DIP‐1 presented a straight hexahedron structure, but no triple‐helical conformation. In antioxidant activity tests, DIP‐1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose‐dependent manner. Especially, DIP‐1 demonstrated high inhibitory activities against SKOV‐3 and Hep‐G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively. Practical applications D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on. At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity. In the present study, we purified and characterized a neutral polysaccharide from D. indica for the first time. Moreover, the neutral polysaccharide exhibits significant antioxidant and antitumor activities. Therefore, the present study laid a foundation for the high‐value application of D. indica polysaccharides in functional food and pharmaceutical industries.

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Section: Introductionmentioning

confidence: 99%

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Xiang

Лі

et al. 2019

J Food Biochem

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“…As a result, we hypothesized that the decreased cell viability and phagocytic capacity of I01-2 and I01-4 with an increase in concentration from 400 μg/mL or 1000 μg/mL, respectively, might be caused by an excessive build-up of NO in macrophages. Additionally, the NO levels in groups receiving EPS treatments were weaker than those in the group receiving LPS treatments, indicating that the EPS effects were more moderate than that of LPS [ 60 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of Novel Exopolysaccharides from Enterococcus hirae WEHI01 and Its Immunomodulatory Activity

Jia

Wei

et al. 2022

Foods

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Exopolysaccharide (EPS) from probiotic Enterococcus hirae WEHI01 was isolated and purified by anion exchange chromatography and gel chromatography, the results of which show that the EPS consists of four fractions, namely I01-1, I01-2, I01-3, and I01-4. As the main purification components, I01-2 and I01-4 were preliminarily characterized for their structure and their immunomodulatory activity was explored. The molecular weight of I01-2 was 2.28 × 104 Da, which consists mainly of galactose, and a few other sugars including glucose, arabinose, mannose, xylose, fucose, and rhamnose, while the I01-4 was composed of galactose only and has a molecular weight of 2.59 × 104 Da. Furthermore, the results of an evaluation of immunomodulatory activity revealed that I01-2 and I01-4 could improve the viability of macrophage cells, improve phagocytosis, boost NO generation, and encourage the release of cytokines including TNF-α and IL-6 in RAW 264.7 macrophages. These results imply that I01-2 and I01-4 could improve macrophage-mediated immune responses and might be useful in the production of functional food and medications.

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“…The prebiotic effect of arabinoxylan oligosaccharide isolated from wheat ( 28 ), antihyperglycemic effects of polysaccharide extracted from Memordica charantia ( 29 ), Inonotus obliquus ( 30 ), Hericium erinaceus ( 31 ), Ophiopogon japonicas ( 32 ), immune-modulatory effect of polysaccharide from Hibiscus sabdariffa L . ( 33 ), antioxidantive potential of polysaccharide from Euryale ferox Salisb. ( 34 ), antitumor effect of polysaccharide from Polygonum multiflorum ( 35 ) and other therapeutic efficacies of polysaccharides and oligosaccharides were investigated in the literature.…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic potential of the isolated fractions from the plants of Rosaceae family in streptozotocin-induced diabetic rats

Bahrami

Izadi

Miraghaee

et al. 2021

Research in Pharmaceutical Sciences

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Background and purpose: Diabetes is a group of multifactorial disorders characterized by chronic-elevated blood glucose levels (hyperglycemia). Natural remedies are used as alternative medications to treat diabetes. Here, we tested the protective effect of the plant extracts of the Rosaceae family on improving insulin secretion and repairing the pancreatic beta cells in diabetic rats. Experimental approach: The oligosaccharide fraction was isolated from the Rosaceae family of herbs. LC-MS/MS was applied to characterize the isolated fractions. The male Wistar rats were randomly divided into six groups, 10 each, including the control group with no intervention, diabetic rats without treatment, diabetic rats that received the extract of Malus domestica (apple), Cydonia oblonga (quince), Prunus persica (nectarine), and Prunus persica (peach), separately. Rats were monitored for the weight, fasting plasma glucose, and insulin levels. The effect of extracts in streptozotocin (STZ)-induced diabetic rats on the pancreatic islets was evaluated by morphometric analysis. Findings/Results: LC-MS/MS results indicated a similar mass spectrum of isolated fractions from nectarine and peach with Rosa canina . Oral administration of nectarine and peach extracts to STZ-induced diabetic rats showed restoration of blood glucose levels to normal levels with a concomitant increase in insulin levels. Morphometric analysis of pancreatic sections revealed the increase in number, diameter, volume, and area of the pancreatic islets in the diabetic rats treated with extracts compared to the untreated diabetic rats. Conclusion and implications: Nectarine and peach extracts’ anti-diabetic properties improved insulin secretion and pancreatic beta-cell function and subsequently led to restoring pancreatic islet mass in STZ-induced diabetic rats.

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Purification, characterization and immunoregulatory activity of a polysaccharide isolated from Hibiscus sabdariffa L.

Cited by 20 publications

References 52 publications

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Characterization of Novel Exopolysaccharides from Enterococcus hirae WEHI01 and Its Immunomodulatory Activity

Antidiabetic potential of the isolated fractions from the plants of Rosaceae family in streptozotocin-induced diabetic rats

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