Purification, characterization and molecular cloning of a novel endo-β-1,4-glucanase AC-EG65 from the mollusc Ampullaria crossean

Purification and characterization of a novel endo-β-1,4-glucanase, AfEG22, from the giant snail, <italic>Achatina fulica frussac</italic>

“…AfEG22 is an enzyme that appears to be optimally adapted to work under acidic conditions as the optimal pH is 4.0. Although it is not clear whether AfEG22 is secreted by the giant snail or its symbiotic microbes, it has maximum activity among animal cellulases under weak acidic conditions [6,7,11,12], meanwhile the optimal pHs of cellulases from other sources vary from acidic to alkaline [27,28]. AfEG22 shows almost acidophilic and alkaliphilic feature, as it could retain more than 80% of its maximum activity when incubating 1 h at pH 11.5 and more than 70% at pH 2 after incubation at 37 for 1 h. The effects of pH on AfEG22 resemble that on EG27, another mollusk-origin cellulase [8].…”

“…A number of laboratories have given great attention to discover novel, more stable, and highly efficient cellulases and hemicellulases to meet industrial requirements. Besides bacteria and fungi, these enzymes have also been found in animals including mollusks [6][7][8], termites [9], urchin [10], abalone [11], blue mussel [12], Ciona intestinalis [13], and corbicula japonica [14,15].…”

Degradation of Lignocellulosic Components in Un-pretreated Vinegar Residue Using an Artificially Constructed Fungal Consortium

“…Xylanase activity was tested by the methodology described by Latif et al (2006). β-glucanase activity was assayed using dextran from leuconostoc as the substrate (Li et al 2009). α-amylase activity was estimated by measuring the maltose content using the method of DNS (Dahlqvist 1962).…”

Evaluation of operational parameters on the precipitation of endoglucanase and xylanase produced by solid state fermentation of Aspergillus niger

“…Li et al (2009) described the purification of a novel endoglucanase from the gastric juice of the mollusc Ampullaria crossean. A recovery of 61.9% was achieved when the enzyme extract was added to 55% saturation of ammonium sulfate and left overnight at 4°C.…”

“…However, most of them describe the precipitation step at a single specific condition (Bokhari et al, 2009;Li et al, 2009;Jabbar et al, 2008;Ninawa et al, 2008;Saha, 2004;Oikawa et al, 1997). Finding the optimum operational condition for precipitation can involve a study of different variables such as the type and concentration of the precipitant agent, temperature, aging time, agitation, pH and so forth.…”