Purification, Characterization and N-terminal Protein Sequencing of the Enzyme Dextransucrase Produced by Leuconostoc mesenteroides

Dawoud, Turki M.; Alshehrei, Fatimah; Siddiqui, Khaizran; Ameen, Fuad; Akhtar, Jameela; Arif, Afsheen

doi:10.13005/bbra/2915

Cited by 4 publications

(2 citation statements)

References 28 publications

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“…With regard to the Mw, the majority of previous zymogram analysis of Leuconostoc Dsr showed appearance of one band with size of 200 kDa (Dawoud et al 2021 ), 180 kDa (Bounaix et al 2010a ) and 170 kDa (Shukla and Goyal 2014 ). According to the complete protein sequences of the GH family 70 on NCBI Database, the highest Mw of extracellular Lc.…”

Section: Discussionmentioning

confidence: 98%

Leuconostoc mesenteroides and Liquorilactobacillus mali strains, isolated from Algerian food products, are producers of the postbiotic compounds dextran, oligosaccharides and mannitol

Zarour,

Zeid,

Mohedano

et al. 2024

World J Microbiol Biotechnol

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Six lactic acid bacteria (LAB) isolated from Algerian sheep’s milk, traditional butter, date palm sap and barley, which produce dextran, mannitol, oligosaccharides and vitamin B2 have been characterized. They were identified as Leuconostoc mesenteroides (A4X, Z36P, B12 and O9) and Liquorilactobacillus mali (BR201 and FR123). Their exopolysaccharides synthesized from sucrose by dextransucrase (Dsr) were characterized as dextrans with (1,6)-d-glucopyranose units in the main backbone and branched at positions O-4, O-2 and/or O-3, with d-glucopyranose units in the side chain. A4X was the best dextran producer (4.5 g/L), while the other strains synthesized 2.1–2.7 g/L. Zymograms revealed that L. mali strains have a single Dsr with a molecular weight (Mw) of ~ 145 kDa, while the Lc. mesenteroides possess one or two enzymes with 170–211 kDa Mw. As far as we know, this is the first detection of L. mali Dsr. Analysis of metabolic fluxes from sucrose revealed that the six LAB produced mannitol (~ 12 g/L). The co-addition of maltose–sucrose resulted in the production of panose (up to 37.53 mM), an oligosaccharide known for its prebiotic effect. A4X, Z36P and B12 showed dextranase hydrolytic enzymatic activity and were able to produce another trisaccharide, maltotriose, which is the first instance of a dextranase activity encoded by Lc. mesenteroides strains. Furthermore, B12 and O9 grew in the absence of riboflavin (vitamin B2) and synthesized this vitamin, in a defined medium at the level of ~ 220 μg/L. Therefore, these LAB, especially Lc. mesenteroides B12, are good candidates for the development of new fermented food biofortified with functional compounds.

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Section: Discussionmentioning

confidence: 98%

Leuconostoc mesenteroides and Liquorilactobacillus mali strains, isolated from Algerian food products, are producers of the postbiotic compounds dextran, oligosaccharides and mannitol

Zarour,

Zeid,

Mohedano

et al. 2024

World J Microbiol Biotechnol

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“…Fungal mycelia grow on the surface of the anode or in the anodic chamber, releasing their degradative and fermentative enzymes. The traditional biological procedure is insufficient in pollutant remediation because of the absence of “biocatalytic activity” ( Dawoud et al, 2021 ; Al-Enazi et al, 2023a , b ). Deposited catalysts have gained significant interest at electrodes, accelerating the kinetics reaction (the electrodes).…”

Section: Fungal Catalytic Role In Fcsmentioning

confidence: 99%

Harnessing fungal bio-electricity: a promising path to a cleaner environment

Umar,

Mubeen,

Ali

et al. 2024

Front. Microbiol.

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Integrating fungi into fuel cell systems presents a promising opportunity to address environmental pollution while simultaneously generating energy. This review explores the innovative concept of constructing wetlands as fuel cells for pollutant degradation, offering a practical and eco-friendly solution to pollution challenges. Fungi possess unique capabilities in producing power, fuel, and electricity through metabolic processes, drawing significant interest for applications in remediation and degradation. Limited data exist on fungi’s ability to generate electricity during catalytic reactions involving various enzymes, especially while remediating pollutants. Certain species, such as Trametes versicolor, Ganoderma lucidum, Galactomyces reessii, Aspergillus spp., Kluyveromyce smarxianus, and Hansenula anomala, have been reported to generate electricity at 1200 mW/m3, 207 mW/m2, 1,163 mW/m3, 438 mW/m3, 850,000 mW/m3, and 2,900 mW/m3, respectively. Despite the eco-friendly potential compared to conventional methods, fungi’s role remains largely unexplored. This review delves into fungi’s exceptional potential as fuel cell catalysts, serving as anodic or cathodic agents to mitigate land, air, and water pollutants while simultaneously producing fuel and power. Applications cover a wide range of tasks, and the innovative concept of wetlands designed as fuel cells for pollutant degradation is discussed. Cost-effectiveness may vary depending on specific contexts and applications. Fungal fuel cells (FFCs) offer a versatile and innovative solution to global challenges, addressing the increasing demand for alternative bioenergy production amid population growth and expanding industrial activities. The mechanistic approach of fungal enzymes via microbial combinations and electrochemical fungal systems facilitates the oxidation of organic substrates, oxygen reduction, and ion exchange membrane orchestration of essential reactions. Fungal laccase plays a crucial role in pollutant removal and monitoring environmental contaminants. Fungal consortiums show remarkable potential in fine-tuning FFC performance, impacting both power generation and pollutant degradation. Beyond energy generation, fungal cells effectively remove pollutants. Overall, FFCs present a promising avenue to address energy needs and mitigate pollutants simultaneously.

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Evaluating the effect of X ray irradiation in the control of food bacterial pathogens

Alanazi

2023

Journal of King Saud University - Science

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Purification, Characterization and N-terminal Protein Sequencing of the Enzyme Dextransucrase Produced by Leuconostoc mesenteroides

Cited by 4 publications

References 28 publications

Leuconostoc mesenteroides and Liquorilactobacillus mali strains, isolated from Algerian food products, are producers of the postbiotic compounds dextran, oligosaccharides and mannitol

Leuconostoc mesenteroides and Liquorilactobacillus mali strains, isolated from Algerian food products, are producers of the postbiotic compounds dextran, oligosaccharides and mannitol

Harnessing fungal bio-electricity: a promising path to a cleaner environment

Evaluating the effect of X ray irradiation in the control of food bacterial pathogens

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