Purification, cloning, expression and immunological analysis of Scylla serrata arginine kinase, the crab allergen

Abstract

Both of purified nAK and rAK reacted positively with sera from subjects with crustacean allergy in immunoblotting and GICA analysis, indicating AK is a common allergen of mud crab. In vitro expressed AK is proposed as a source of the protein for immunological or clinical studies.

Mapping and characterization of antigenic epitopes of arginine kinase of Scylla paramamosain

“…In recent years, other proteins in shellfish, such as myosin light chain, sarcoplasmic calcium-binding protein, troponin C, and triosephosphate isomerase, have been identified as novel allergenic proteins (Bauermeister et al, 2011). The 40-kDa arginine kinase (AK) from the shrimps Penaeus monodon (Yu et al, 2003) and Penaeus (Litopenaeus) vannamei (Garcia-Orozco et al, 2007) and from the Indo-Pacific swamp crab Scylla serrata (Shen et al, 2011) has also been identified as an important allergen.…”

“…We have previously shown that AK is specifically recognized by IgE collected from crab-sensitized allergic patients (Shen et al, 2011), and have defined two areas of S. paramamosain AK (amino acids 174-181 and 253-256) as the main epitope regions of the protein. Further serological assays of synthetic peptides defined three experimental linear epitopes (amino acids 127-141, 141-155, and 211-225) .…”

Arginine kinase in the cladoceran Daphnia magna: cDNA sequencing and expression is associated with resistance to toxic Microcystis

“…Several AKs were cloned or identified from other crustaceans (Arockiaraj et al, 2011;Shen et al, 2011;Chen et al, 2013;Xie et al, 2014). In this study, an AK gene was cloned from D. magna with an ORF of 1074 bp encoding for 357 AA.…”

Characterization of Seafood Proteins Causing Allergic Diseases

“…AK was recently reported as an allergen in different crustacean species which was identified in white shrimp (Litopenaeus vannamei) (García-Orozco et al, 2007), gulf shrimp (Penaeus aztecus) (France et al, 1997), chinese shrimp (Fenneropenaeus chinensis) ( Y a o et al, 2005), black tiger prawn (Penaeus monodon) and other shrimp species using a proteomics approach (Abdel Rahman et al, 2010a;Yu et al, 2003). Moreover, AK has been identified in other crab species: mud crab (Scylla serrata) (Shen et al, 2011), and by our group in snow crab (Chionoecetes opilio) (Abdel Rahman et al, 2011), where 49% of the participant patient's sera have a reactivity with AK. Arginine kinase also has been reported is allergen in some other invertebrates, such as the house dust mite (Dermatophagoides farinae) (Bi & Chew, 2004), Indian-meal moth (Plodia interpunctella) (Binder et al, 2001), and silkworm larvae (Bombyx mori) (Liu et al, 2009).…”

“…Allergen analysis by MS started by determining the molecular weight of small allergens such as parvalbumin (12 kDa) in fish (Bugajska-Schretter et al, 2000;Cai et al, 2010;Swoboda et al, 2002). After completing the genome sequence and developing the MS-interface databases, some studies use the PMF approach to identify the seafood allergens evaluated by immunoblotting (Ayuso et al, 2009;García-Orozco et al, 2007;Mazzeo et al, 2008;Shen et al, 2011;Yamasaki et al, 2010;Yu et al, 2003). The best example of this kind of research was in 2003, when Pen m 2 shrimp allergen was identified using PMF, where the recognized spot proteins by a pool of patients' sera were tryptic digested and analyzed by MALDI-ToF (Yu et al, 2003).…”