Purification, composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl.

Luo, Aoxue; He, Xing‐Jin; Zhou, Song‐Dong; Fan, Yujiang; Luo, A-Li; Chun, Ze

doi:10.1016/j.carbpol.2009.10.033

Cited by 242 publications

(126 citation statements)

References 25 publications

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“…-The antioxidant activity was determined using ABTS radical cation measurements (6)(7)(8), with some modifications. ABTS radical cations were produced by reacting 7 mmol L -1 ABTS solution with 2.45 mmol L -1 potassium persulfate.…”

Section: Antioxidant Activitymentioning

confidence: 99%

Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology

Lan

Lin

2014

Acta Pharmaceutica

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Evaluation of the antioxidant activity of Coreopsis tinctoria Nuff. and optimisation of isolation by response surface methodologyThe aim of the present study was to determine the antioxidant activity of Coreopsis tinctoria flowering tops (CTFT). Studies were conducted to obtain suitable extraction conditions for chlorogenic acid, quercetin, luteolin, apigenin and kaempferol, which were identified and quantified by HPLC. Response surface methodology was employed to optimise the ultrasound-assisted extraction conditions including extraction time, ethanol concentration and liquid-solid ratio. The antioxidant activity of the extracts was analysed using various antioxidant models, such as DPPH, ABTS and hydroxyl radical scavenging assay. CTFT extracted for 15.0 min with ethanol at a concentration of 60.4 % and with liquidsolid ratio 27.5:1 possessed a considerable amounts of total flavonoids and polyphenols (18.9 %). This extract showed higher scavenging activity of ABTS and hydroxyl radical activity than rutin, however not in the DPPH test. We may assume that CTFT possess antioxidant and free radical scavenging potentials.

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Section: Antioxidant Activitymentioning

confidence: 99%

Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology

Lan

Lin

2014

Acta Pharmaceutica

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“…The ABTS + assay can be used as an index to reflect the antioxidant activity of the test samples [26]. The scavenging ability of SJP extract and the purified SJP on ABTS free radicals increased obviously (p < 0.05) with the increasing concentration presented in Figure 4C.…”

Section: Abts Scavenging Activitymentioning

confidence: 94%

Optimization Extraction, Preliminary Characterization and Antioxidant Activities of Polysaccharides from Semen Juglandis

Ren

Wang

et al. 2016

Molecules

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Abstract:The optimization extraction process, preliminary characterization and antioxidant activities of polysaccharides from Semen Juglandis (SJP) were studied in this paper. Based on the Box-Behnken experimental design and response surface methodology, the optimal extraction conditions for the SJP extraction were obtained as follows: temperature 88 • C, extraction time 125 min and ratio of liquid to solid 31 mL/g. Under these conditions, experimental extraction yield of SJP was (5.73 ± 0.014)% (n = 5), similar to the predicted value of 5.78%. Furtherly, the purified SJP obtained from SJP extract by DEAE-52 and Sephacryl S-100 chromatography was analyzed to be rhamnose, galacturonic acid, galactose, arabinose and fucose in the molar ratio of 1:6.34:1.38:3.21:1.56. And the weight-average molecular weight and radius of gyration of the purified SJP in 0.1 M NaCl were determined to be 2.76 × 10 4 g/mol and 122 nm by SEC-MALLS, respectively. More importantly, it exhibited appreciable antioxidant activities compared to the standard Vc, such as DPPH radical scavenging activity (IC 50 0.21 mg/mL), strong reducing power, ABTS radical scavenging activity (IC 50 0.29 mg/mL), and hydroxyl radical scavenging activity (IC 50 0.38 mg/mL). These results indicate that SJP may be useful for developing functional health products or natural antioxidant.

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“…Preparation of the crude polysaccharide (PEPW80) from P. emblica was performed by water extraction and ethanol precipitation (27). P. emblica powder was extracted with 95% ethanol and then with distilled water for 2 h at 80°C three times.…”

Section: Preparation and Isolation Of Polysaccharidesmentioning

confidence: 99%

Structural characterization and biological activities of a novel polysaccharide from <i>Phyllanthus emblica </i>

Zeng

Lv²,

Jing³

et al. 2017

DD&T

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Purification, composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl.

Cited by 242 publications

References 25 publications

Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology

Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology

Optimization Extraction, Preliminary Characterization and Antioxidant Activities of Polysaccharides from Semen Juglandis

Structural characterization and biological activities of a novel polysaccharide from <i>Phyllanthus emblica </i>

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