Purification, compositional analysis, and anticoagulant capacity of chondroitin sulfate/dermatan sulfate from bone of corb (Sciaena umbra)

Bougatef, Hajer; Krichen, Fatma; Capitani, Federica; Amor, Ikram Ben; Gargouri, Jalel; Maccari, Francesca; Mantovani, Veronica; Galeotti, Fabio; Volpi, Nicola; Bougatef, Ali; Sila, Assaâd

doi:10.1016/j.ijbiomac.2019.05.036

Cited by 21 publications

(17 citation statements)

References 42 publications

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“…Overall, 85% ethanol-extracted method can be used to obtain ethanol-soluble component without chondroitin sulfate. It has been reported that chondroitin sulfate extracted from the cartilage of smooth-hound, corb (Sciaena umbra) and sturgeon (Acipenser sinensis) had the characteristic absorption peaks of polysaccharides at 200, 210-215, and 206 nm in UV spectrum, respectively (46,48,49). However, ESCH did not show an absorption peak at 200 nm (Figure 1A), which confirmed the electrophoresis result (Figure 1B).…”

Section: Discussionsupporting

confidence: 78%

“…Therefore, hot-pressure combined with double enzyme hydrolysis can be an effective method to promote the release of small molecular peptides from cartilage. Agarose-gel electrophoresis is used to detect the residue of chondroitin sulfate (46,47). Chondroitin sulfate usually exists in the form of proteoglycan.…”

Section: Discussionmentioning

confidence: 99%

“…Chondroitin sulfate from different sources has different molecular weights. The molecular weight of chondroitin sulfate isolated from chicken, bony fishes and cartilaginous fish were 30-37.18 kDa (32, 33), 13.46-48.68 kDa (46,47), and 50-70 kDa (48), respectively. It was found that the molecular weight of chondroitin sulfate obtained in sturgeon was at least 10 kDa, which was consistent with previous reports.…”

Section: Discussionmentioning

confidence: 99%

“…It implied that there may be no free acid group. Values of 1,240-925 cm −1 are the characteristic absorption of D-glucopyranose ring and sulfate groups (31,40,46,48), which descripts the exitance of carbohydrates in ESCH. The characteristic stretching vibration peak of the axial coordination of C-O-S in ESCH appears at 867.34 cm −1 .…”

Section: Discussionmentioning

confidence: 99%

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Anti-inflammatory and Antioxidant Activity of Peptides From Ethanol-Soluble Hydrolysates of Sturgeon (Acipenser schrenckii) Cartilage

Chu

et al. 2021

Front. Nutr.

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Research has shown that cartilage containing chondroitin sulfate and protein presents versatile bioactivities. Chondroitin sulfate in cartilage is beneficial to activate the immune system while the protein/peptide has not been fully understood. The current study investigated the antioxidant and anti-inflammatory properties of ethanol-soluble hydrolysates of sturgeon cartilage (ESCH) prepared through hot-pressure, enzymatic hydrolysis and ethanol extraction. UV spectrum, IR and agarose gel electrophoresis results suggested the successful exclusion of chondroitin sulfate from peptides. Nitric oxide (NO) floods in cells activated by inflammation. It was inhibited when administrated with ESCH. To further explain the observed anti-inflammatory activity, ESCH was separated with Sephadex G-15 into 3 components, among which F3 showed a higher NO inhibition rate and significantly reduced the production of the proinflammatory cytokine IL-6. In addition, the yield of IL-10 increased. Western blotting suggested that F3 downregulated the NO content and IL-6 level by suppressing Mitogen-activated protein kinases (MAPK) channels. Moreover, both ESCH and F3 showed DPPH and ABTS free radical scavenging abilities which was possibly related to the anti-inflammatory property. These results indicated that ESCH behaved anti-inflammatory and antioxidant activities. Cartilage may be a good source to produce anti-inflammatory peptides.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Anti-inflammatory and Antioxidant Activity of Peptides From Ethanol-Soluble Hydrolysates of Sturgeon (Acipenser schrenckii) Cartilage

Chu

et al. 2021

Front. Nutr.

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“…Thus, CBG shows an anticoagulation effect mediated by both an intrinsic and an extrinsic coagulation cascade. The presence of disulfation in the disaccharide at IdoA/GlcA2S and GalNAc4S, or at IdoA/GlcA4S and GalNAc6S, appeared to corroborate the inhibitory thrombin activity of this marine GAG [ 56 ].…”

Section: Marine Antithromboticsmentioning

confidence: 99%

Marine Antithrombotics

Dwivedi

Pomin

2020

Marine Drugs

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Thrombosis remains a prime reason of mortality worldwide. With the available antithrombotic drugs, bleeding remains the major downside of current treatments. This raises a clinical concern for all patients undergoing antithrombotic therapy. Novel antithrombotics from marine sources offer a promising therapeutic alternative to this pathology. However, for any potential new molecule to be introduced as a real alternative to existing drugs, the exhibition of comparable anticoagulant potential with minimal off-target effects must be achieved. The relevance of marine antithrombotics, particularly sulfated polysaccharides, is largely due to their unique mechanisms of action and lack of bleeding. There have been many investigations in the field and, in recent years, results have confirmed the role of potential marine molecules as alternative antithrombotics. Nonetheless, further clinical studies are required. This review covers the core of the data available so far regarding the science of marine molecules with potential medical applications to treat thrombosis. After a general discussion about the major biochemical steps involved in this pathology, we discuss the key structural and biomedical aspects of marine molecules of both low and high molecular weight endowed with antithrombotic/anticoagulant properties.

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