Purification, crystallization and preliminary crystallographic studies on 2-dehydro-3-deoxygalactarate aldolase fromLeptospira interrogans

Abstract

2-Dehydro-3-deoxygalactarate (DDG) aldolase is a member of the class II aldolase family and plays an important role in the pyruvate-metabolism pathway, catalyzing the reversible aldol cleavage of DDG to pyruvate and tartronic semialdehyde. As it is a potential novel antibiotic target, it is necessary to elucidate the catalytic mechanism of DDG aldolase. To determine the crystal structure, crystals of DDG aldolase from Leptospira interrogans were obtained by the hanging-drop vapour-diffusion method. The crystals diffracted to 2.2 Å resolution using a Cu K rotating-anode X-ray source. The crystal belonged to space group C2, with unit-cell parameters a = 293.5, b = 125.6, c = 87.6 Å , = 100.9 . The V M is calculated to be 2.4 Å 3 Da À1 , assuming there to be 12 protein molecules in the asymmetric unit.