Purification of Soybean Phosphatidylcholine Using D113‐III Ion Exchange Macroporous Resin Packed Column Chromatography

Liu, Yuanfa; Liang, Shan; Wang, Xingguo

doi:10.1007/s11746-008-1324-9

Cited by 18 publications

(8 citation statements)

References 16 publications

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“…In an alkaline environment (pH [ 7), excessive Na ? may compete with L-a-GPC for adsorption, and the alkaline environment might cause damage to the resin, which is consistent with previous results [19,20]. From the above results, the L-a-GPC solution was kept at pH 7 prior to chromatographic adsorption.…”

Section: Effect Of Ph Of L-a-gpc Solution On the Adsorption Ratiosupporting

confidence: 91%

Purification of l‐α‐Glycerylphosphorylcholine from the Enzyme Reaction Solutions by Resin Column Chromatography

Zhang

Wang

Liu

2012

J Americ Oil Chem Soc

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Colorless L-a-glycerylphosphorylcholine (L-a-GPC) was obtained at 98.8% purity, and with a specific rotation of -2.5°via a four-step procedure. L-a-GPC was first produced by phospholipase A 1 hydrolysis of phosphatidylcholine in an aqueous medium. Almost all the impurities were then removed using two successive D001 cation and D301-111 anion exchange resin column chromatography procedures, followed by decoloration with active carbon to remove minor pigments and remaining impurities. D001 resin and D301-111 resin showed remarkable ability for L-a-GPC isolation after being used eight times. Thus, this study presents a simple and cost-effective method for preparing L-a-GPC with high purity, low costs, and environmental friendliness, and encourages future investigation into its adaptation for industrial applications.

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Section: Effect Of Ph Of L-a-gpc Solution On the Adsorption Ratiosupporting

confidence: 91%

Purification of l‐α‐Glycerylphosphorylcholine from the Enzyme Reaction Solutions by Resin Column Chromatography

Zhang

Wang

Liu

2012

J Americ Oil Chem Soc

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“…PC, PE, and PI, the most important PLs in lecithin, have different head groups that endow them with different polarities and solubilities in organic solvents, and thus with different emulsification properties . Utilizing these features, several methods, such as extraction, precipitation, supercritical carbon dioxide purification, and column chromatographic purification, have been used for the fractionation of PC from soybean and egg yolk lecithin . However, those methods are frequently expensive, time consuming, difficult for the recovery, reutilization of solvents, etc.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous extraction of phosphatidylcholine and phosphatidylethanolamine from soybean lecithin

John

Park

Lee

et al. 2015

Euro J Lipid Sci & Tech

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A high composition of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was simultaneously extracted from soybean lecithin by using a mixed solvent consisting of ethanol and chloroform. The addition of chloroform in ethanol improved the total extraction yield to give high a composition of PC and PE. Thus, the best extraction yield (52%) of PC and PE was achieved at an ethanol/chloroform ratio of 6/4 (v/v). Molecular dynamics calculations of the amorphous cell bearing PC, PE, and solvent molecules were performed in order to understand the distribution of PC and PE molecules in specific solvent conditions. The extraction yield was also sensitive to extraction temperature, time and liquid to solid ratio. The extracted PC and PE mixture was successfully purified by flash chromatography using chloroform/methanol (8/2 v/v) as eluting solvent. The purified yields of PE and PC from lecithin extract were 16 and 35%, respectively. The structure of the extracted products was characterized by thin-layer chromatography and 1 H, 13 C, 31 P NMR, FTIR and UV/vis spectroscopies, and elemental analysis. The purity of PE and PC was higher than 95%.Practical applications: Lecithin composed of phosphoric acid, choline, fatty acids, glycerol, glycolipids, triglycerides, and phospholipids is widely used in human food, animal feed, pharmaceuticals, paints, and other industrial applications. Lecithin can easily be extracted chemically using various solvents in order to obtain pure components. Due to the importance of phospholipids there have been a number of reports on the extraction of PC from lecithin; however, there have been few reports on the extraction of PE or the simultaneous extraction of PC and PE. Thus the simultaneous extraction of PC (35%) and PE (16%) from soybean lecithin by using a mixed solvent consisting of ethanol and chloroform can be a good example to obtain such components in high yields. The protocol employed here is quite straightforward so that pure PC and PE can be obtained economically and may attract interest on mixed solvent systems for the simultaneous extraction of lecithin components.

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“…However, no effective phenol extraction and purification is possible from such complex and heterogeneous matrices, which have to be disposed as solid waste or can eventually combusted for energy production [17]. The use of macroporous resins [19,20], however, offers significant advantages over many other conventional methods, including a minimum of a 10-fold increase in sample loading capacity, higher selectivity, easier desorption, lower solvent consumption, absence of chemical residues in the product, better mechanical strength and ability to reuse and also improved cost efficiency [21][22][23][24]. The pH of the solution constitutes a parameter that strongly influences the adsorption capacity of a macroporous resin, due to its influence on the adsorption mechanisms.…”

Section: Desalination and Water Treatmentmentioning

confidence: 99%

A study on the kinetics of olive mill wastewater (OMWW) polyphenols adsorption on the commercial XAD4 macroporous resin

Petrotos

Gkoutsidis

Kokkora

et al. 2013

Desalination and Water Treatment

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A B S T R A C TThe commercially available macroporous resin XAD4 was evaluated for its capacity to adsorb the polyphenols contained in olive mill wastewater (OMWW). The adsorption was performed in the mode of fixed packed bed contactor. The effects of OMWW flow rate, temperature, dilution and pH on the resin adsorption capacity were investigated. Polyphenols concentration decreased with time. The pattern of the total polyphenols concentration dependence on time was found similar in all cases. The pattern included a very steep section for roughly the first 2 h of the operation, followed by a second section of decreasing gradient down to a final asymptotic equilibrium limit. Neither the time required for approaching the equilibrium nor the magnitude of the equilibrium concentration was substantially affected by the OMWW temperature. The increase of OMWW flow rate affected markedly the speed of approaching the equilibrium but not its position. The variation of the total polyphenols concentration showed that higher concentrations yielded more rapid kinetics at the initial section, but no apparent differentiation of the time was required to approach equilibrium. Acidic pH appeared to be favourable for the adsorption. Finally, a novel modelling approach was developed to simulate the adsorption process kinetics.

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Purification of Soybean Phosphatidylcholine Using D113‐III Ion Exchange Macroporous Resin Packed Column Chromatography

Cited by 18 publications

References 16 publications

Purification of l‐α‐Glycerylphosphorylcholine from the Enzyme Reaction Solutions by Resin Column Chromatography

Purification of l‐α‐Glycerylphosphorylcholine from the Enzyme Reaction Solutions by Resin Column Chromatography

Simultaneous extraction of phosphatidylcholine and phosphatidylethanolamine from soybean lecithin

A study on the kinetics of olive mill wastewater (OMWW) polyphenols adsorption on the commercial XAD4 macroporous resin

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