Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata

Gao, Na; Chen, Ru; Mou, Rongrong; Xiang, Jingying; Zhou, Kai; Li, Zi; Zhao, Jinhua

doi:10.1016/j.ijbiomac.2020.08.150

Cited by 39 publications

(28 citation statements)

References 32 publications

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“…After being subjected to a Shodex Ohpak SB-804 HQ column, the HPSEC-MALLS-RID of NFG showed only one peak, while NFG-2.0M showed two peaks ( Figure 2 ). As shown in Figure 2 B, NFG-2.0M appeared to contain NFG (peak 2) and a small amount of sulfated fucan SF-II (peak 1) according to the studies reported previously [ 20 ]. The

and the polydispersity (

) of NFG and NFG-2.0M by HPSEC-MALLS-RID (only the NFG peak was involved in the calculation) are presented in Table 2 .…”

Section: Resultssupporting

confidence: 84%

“…Meanwhile, the RSD of stability (0 h, 4 h, 8 h, 12 h, 24 h at room temperature) for NFG solutions was 1.27% (n = 5), which indicates that NFG was stable in 0.1 mol/L sodium nitrate (containing 0.02% sodium azide) at room temperature during the tested period. It was found that the

and the

of the NFG peak (peak 2) of the NFG-2.0M sample could be not precisely determined due to the relatively poor resolution of the column and the co-elution of the sulfated fucan SF-II (peak 1) [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

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Critical Quality Control Methods for a Novel Anticoagulant Candidate LFG-Na by HPSEC-MALLS-RID and Bioactivity Assays

Zheng

Wang

et al. 2022

Molecules

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A low molecular weight fucosylated glycosaminoglycan sodium (LFG-Na) is a novel anticoagulant candidate from the sea cucumber Holothuria fuscopunctata that selectively inhibits intrinsic tenase (iXase). The molecular weight, molecular weight distribution and bioactivities are the critical quality attributes of LFG-Na. The determination of these quality attributes of such an oligosaccharides mixture drug is challenging but critical for the quality control process to ensure its safety and efficacy in clinical use. Herein, the molecular weight and molecular weight distribution of LFG-Na were successfully determined using high performance size exclusion chromatography coupled with multi angle laser light scattering and refractive index detector (HPSEC-MALLS-RID). Comparing to the conventional method, HPSEC-MALLS-RID based on the refractive index increment (dn/dc) did not require the reference substances to establish the calibration curve. The acceptance criteria of LFG-Na were established, the weight-average molecular weight (Mw) should be 4000 to 6000 Da, the polydispersity (Mw/Mn) < 1.40, and the fraction with molecular weights of 1500 to 8000 Da should be no less than 80% of the total. HPSEC-MALLS-RID was also utilized for the determination of the starting material native fucosylated glycosaminoglycan (NFG) to choose a better manufacturing process. Furthermore, APTT assay was selected and the potency of anti-iXase, referring to the parallel line assay (PLA) method, was established to clarify the consistency of its biological activities. The results suggest that HPSEC-MALLS-RID and bioactivity assays are critical quality control methods for multi-component glycosaminoglycan LFG-Na. The methods also provide a feasible strategy to control the quality of other polysaccharide medicines.

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and the polydispersity (

) of NFG and NFG-2.0M by HPSEC-MALLS-RID (only the NFG peak was involved in the calculation) are presented in Table 2 .…”

Section: Resultssupporting

confidence: 84%

and the

Section: Resultsmentioning

confidence: 99%

Critical Quality Control Methods for a Novel Anticoagulant Candidate LFG-Na by HPSEC-MALLS-RID and Bioactivity Assays

Zheng

Wang

et al. 2022

Molecules

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“…Sulfated polysaccharides (SPs) are sulfated derivatives of polysacc multifunctional active compounds formed by substituting some hydrox osaccharide molecules in the chain of polysaccharides by sulfate radi special environment of the sea, polysaccharides from marine organism more sulfate groups than polysaccharides from terrestrial microorgani plants [8,9]. A variety of MSPs have been isolated from seaweed and ma as fucosylated chondroitin sulfate, chondroitin sulfate, fucoidan, kerata sulfate, carrageenans, and ulvans [10,11]. The diverse biological activitie ing antiobesity, anticoagulant, immunomodulating, antihyperlipidem tive, have attracted remarkable interest.…”

Section: Introductionmentioning

confidence: 99%

“…Due to the special environment of the sea, polysaccharides from marine organisms tend to contain more sulfate groups than polysaccharides from terrestrial microorganisms, animals, and plants [8,9]. A variety of MSPs have been isolated from seaweed and marine animals, such as fucosylated chondroitin sulfate, chondroitin sulfate, fucoidan, keratan sulfate, heparin sulfate, carrageenans, and ulvans [10,11]. The diverse biological activities of MSPs, including antiobesity, anticoagulant, immunomodulating, antihyperlipidemic and antioxidative, have attracted remarkable interest.…”

Section: Introductionmentioning

confidence: 99%

Marine Sulfated Polysaccharides: Preventive and Therapeutic Effects on Metabolic Syndrome: A Review

et al. 2021

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Metabolic syndrome is the pathological basis of cardiovascular and cerebrovascular diseases and type 2 diabetes. With the prevalence of modern lifestyles, the incidence of metabolic syndrome has risen rapidly. In recent years, marine sulfate polysaccharides (MSPs) have shown positive effects in the prevention and treatment of metabolic syndrome, and they mainly come from seaweeds and marine animals. MSPs are rich in sulfate and have stronger biological activity compared with terrestrial polysaccharides. MSPs can alleviate metabolic syndrome by regulating glucose metabolism and lipid metabolism. In addition, MSPs prevent and treat metabolic syndrome by interacting with gut microbiota. MSPs can be degraded by gut microbes to produce metabolites such as short chain fatty acids (SCFAs) and free sulfate and affect the composition of gut microbiota. The difference between MSPs and other polysaccharides lies in the sulfation pattern and sulfate content, therefore, which is very important for anti-metabolic syndrome activity of MSPs. This review summarizes the latest findings on effects of MSPs on metabolic syndrome, mechanisms of MSPs in treatment/prevention of metabolic syndrome, interactions between MSPs and gut microbiota, and the role of sulfate group and sulfation pattern in MSPs activity. However, more clinical trials are needed to confirm the potential preventive and therapeutic effects on human body. It may be a better choice to develop new functional foods containing MSPs for dietary intervention in metabolic syndrome.

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“…Recently some evidence has appeared on the possible presence of several structurally different FS in the same holothurian species. Thus, Holothuria fuscopunctata contains, in addition to 4-linked polymer of α-L-fucose 3-sulfate, another FS with tetrasaccharide repeating units -3)-α-L-Fuc2S,4S-(1-4)-α-L-Fuc-(1-3)-α-L-Fuc2S-(1-4)-α-L-Fuc-(1-with alternating 1-3 and 1-4 interfucoside linkages [36]. A new branched FS was isolated from Pattalus mollis.…”

Section: Introductionmentioning

confidence: 99%

Fucose-Rich Sulfated Polysaccharides from Two Vietnamese Sea Cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera: Structures and Anticoagulant Activity

et al. 2022

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Fucosylated chondroitin sulfates (FCSs) FCS-BA and FCS-HS, as well as fucan sulfates (FSs) FS-BA-AT and FS-HS-AT were isolated from the sea cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera, respectively. Purification of the polysaccharides was carried out by anion-exchange chromatography on DEAE-Sephacel column. Structural characterization of polysaccharides was performed in terms of monosaccharide and sulfate content, as well as using a series of non-destructive NMR spectroscopic methods. Both FCSs were shown to contain a chondroitin core [→3)-β-d-GalNAc-(1→4)-β-d-GlcA-(1→]n bearing sulfated fucosyl branches at O-3 of every GlcA residue in the chain. These fucosyl residues were different in pattern of sulfation: FCS-BA contained Fuc2S4S, Fuc3S4S and Fuc4S at a ratio of 1:8:2, while FCS-HS contained these residues at a ratio of 2:2:1. Polysaccharides differed also in content of GalNAc4S6S and GalNAc4S units, the ratios being 14:1 for FCS-BA and 4:1 for FCS-HS. Both FCSs demonstrated significant anticoagulant activity in clotting time assay and potentiated inhibition of thrombin, but not of factor Xa. FS-BA-AT was shown to be a regular linear polymer of 4-linked α-L-fucopyranose 3-sulfate, the structure being confirmed by NMR spectra of desulfated polysaccharide. In spite of considerable sulfate content, FS-BA-AT was practically devoid of anticoagulant activity. FS-HS-AT cannot be purified completely from contamination of some FCS. Its structure was tentatively represented as a mixture of chains identical with FS-BA-AT and other chains built up of randomly sulfated alternating 4- and 3-linked α-L-fucopyranose residues.

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Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata

Cited by 39 publications

References 32 publications

Critical Quality Control Methods for a Novel Anticoagulant Candidate LFG-Na by HPSEC-MALLS-RID and Bioactivity Assays

Critical Quality Control Methods for a Novel Anticoagulant Candidate LFG-Na by HPSEC-MALLS-RID and Bioactivity Assays

Marine Sulfated Polysaccharides: Preventive and Therapeutic Effects on Metabolic Syndrome: A Review

Fucose-Rich Sulfated Polysaccharides from Two Vietnamese Sea Cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera: Structures and Anticoagulant Activity

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