Purine Photoproducts*

Duker, Nahum J.; Gallagher, Patricia E.

doi:10.1111/j.1751-1097.1988.tb02783.x

Cited by 30 publications

(2 citation statements)

References 31 publications

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“…CPDs, characterized by covalent linkage between adjacent pyrimidines and (6-4) pyrimidone photoproducts (6–4 PPs), several purine photoproducts have also been reported. These include the adenine dimer, the Porschke photoproduct and the T-A photoadduct ( 79 , 81 , 82 ). Accordingly, we found that processes, which are involved in synthesis and uptake of nucleotides, are strongly affected upon UV treatment in the MW001 + pyrEF strain and the tfb3::pyrEF strain.…”

Section: Discussionmentioning

confidence: 99%

Effect of UV irradiation on Sulfolobus acidocaldarius and involvement of the general transcription factor TFB3 in the early UV response

Schult

Albersmeier

et al. 2018

Nucleic Acids Research

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Exposure to UV light can result in severe DNA damage. The alternative general transcription factor (GTF) TFB3 has been proposed to play a key role in the UV stress response in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius. Reporter gene assays confirmed that tfb3 is upregulated 90–180 min after UV treatment. In vivo tagging and immunodetection of TFB3 confirmed the induced expression at 90 min. Analysis of a tfb3 insertion mutant showed that genes encoding proteins of the Ups pili and the Ced DNA importer are no longer induced in a tfb3 insertion mutant after UV treatment, which was confirmed by aggregation assays. Thus, TFB3 plays a crucial role in the activation of these genes. Genome wide transcriptome analysis allowed a differentiation between a TFB3-dependent and a TFB3-independent early UV response. The TFB3-dependent UV response is characterized by the early induction of TFB3, followed by TFB3-dependent expression of genes involved in e.g. Ups pili formation and the Ced DNA importer. Many genes were downregulated in the tfb3 insertion mutant confirming the hypothesis that TFB3 acts as an activator of transcription. The TFB3-independent UV response includes the repression of nucleotide metabolism, replication and cell cycle progression in order to allow DNA repair.

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Section: Discussionmentioning

confidence: 99%

Effect of UV irradiation on Sulfolobus acidocaldarius and involvement of the general transcription factor TFB3 in the early UV response

Schult

Albersmeier

et al. 2018

Nucleic Acids Research

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“…Although dipyrimidine photoproducts are the preferential outcome of UV-B radiation, the biological importance of UV radiation-induced modifications of DNA purine bases has also been recognized [ 64 ]. These comprise the photoproducts that involve, at least, one adenine residue that undergoes photocycloaddition reactions with contiguous adenine or thymine ( Figure 6 ) upon exposure to UV-B radiation [ 65 , 66 ].…”

Section: Uv-induced Purine Photoproductsmentioning

confidence: 99%

Molecular Mechanisms of Ultraviolet Radiation‐Induced DNA Damage and Repair

Rastogi

Richa

Kumar

et al. 2010

Journal of Nucleic Acids

971

735

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DNA is one of the prime molecules, and its stability is of utmost importance for proper functioning and existence of all living systems. Genotoxic chemicals and radiations exert adverse effects on genome stability. Ultraviolet radiation (UVR) (mainly UV-B: 280–315 nm) is one of the powerful agents that can alter the normal state of life by inducing a variety of mutagenic and cytotoxic DNA lesions such as cyclobutane-pyrimidine dimers (CPDs), 6-4 photoproducts (6-4PPs), and their Dewar valence isomers as well as DNA strand breaks by interfering the genome integrity. To counteract these lesions, organisms have developed a number of highly conserved repair mechanisms such as photoreactivation, base excision repair (BER), nucleotide excision repair (NER), and mismatch repair (MMR). Additionally, double-strand break repair (by homologous recombination and nonhomologous end joining), SOS response, cell-cycle checkpoints, and programmed cell death (apoptosis) are also operative in various organisms with the expense of specific gene products. This review deals with UV-induced alterations in DNA and its maintenance by various repair mechanisms.

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Nucleic Acids: Chemical Damage

Friedberg

Kodadek

2008

Wiley Encyclopedia of Chemical Biology

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Nucleic acids are highly reactive macromolecules that can undergo a variety of chemical changes that may be deleterious to their function. In particular, deoxyribonucleic acid (DNA) undergoes multiple spontaneous chemical changes and is reactive with reactive oxygen species generated during oxidative metabolism and with naturally occurring chemicals, ultraviolet radiation, and a myriad of environmental chemicals. This review highlights many of these chemical changes.

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Purine Photoproducts*

Cited by 30 publications

References 31 publications

Effect of UV irradiation on Sulfolobus acidocaldarius and involvement of the general transcription factor TFB3 in the early UV response

Effect of UV irradiation on Sulfolobus acidocaldarius and involvement of the general transcription factor TFB3 in the early UV response

Molecular Mechanisms of Ultraviolet Radiation‐Induced DNA Damage and Repair

Nucleic Acids: Chemical Damage

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