Purple acid phosphatase 10c modifies the rice rhizobacterial community and its phosphorus cycling potential

Abstract

Background and AimesPlant roots secret acid phosphatases (ACPs) to mineralize the rhizosphere organic phosphorus (Po) for absorption. However, the ecological effects of such exudates on the rhizobacterial community and function remain unclear. • MethodsPurple acid phosphatase 10c (OsPAP10c) is the major root-secreted ACP under both Pi-su cient and de cient conditions in rice. To study the effect of rootsecreted ACP on the rhizosphere phosphorus (P) cycling and succession of the bacterial community, the previously reported OsPAP10cmutant and overexpression lines were planted in paddy soils with or without P fertilizer (+P or -P). • ResultsThe results showed that the expression of OsPAP10c signi cantly in uenced ACP activity in rhizosphere soil, which changed bacterial composition by 6.72% to 9.54%. The expression of OsPAP10c helped to recruit P solubilizing bacteria (PSB) under -P conditions. However, OsPAP10c increased bacterial P competition potentials and rhizosphere alkali phosphatase (ALP) under +P conditions. Moreover, overexpression of OsPAP10c increased the relative abundance of bacteria with nitrogen, sulfur, and iron potential functions, which bene t the rice nutrient uptake and growth. • ConclusionIn conclusion, this study indicated that P fertilizer and root-secreted ACPs cooperatively regulate rhizosphere P cycling potential by modifying the rhizobacterial composition.