Putative carotenoid genes expressed under the regulation of Shine–Dalgarno regions in Escherichia coli for efficient lycopene production

Jin, Weiyue; Xu, Xing‐You; Jiang, Ling; Zhang, Zhidong; Li, Shuang; Huang, He

doi:10.1007/s10529-015-1922-1

Cited by 23 publications

(19 citation statements)

References 22 publications

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“…[32b] Finding an www.advancedsciencenews.com optimal media was also important to enhance the production. [91] In cases of fed-batch fermentation, terpenoids production has been reported to change over different auxiliary carbon sources. The engineered E. coli strain introduced with the entire MEV pathway genes performed the best with glycerol as a main carbon source for the production of lycopene.…”

Section: Optimization Of Fermentation Conditionmentioning

confidence: 99%

Metabolic Engineering of Microorganisms for the Production of Natural Compounds

Park

Yang

et al. 2017

Adv. Biosys.

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Natural products have been attracting much interest around the world for their diverse applications, especially in drug and food industries. Plants have been a major source of many different natural products. However, plants are affected by weather and environmental conditions and their successful extraction is rather limited. Chemical synthesis is inefficient due to the complexity of their chemical structures involving enantioselectivity and regioselectivity. For these reasons, an alternative means of overproducing valuable natural products using microorganisms has emerged. In recent years, various metabolic engineering strategies have been developed for the production of natural products by microorganisms. Here, the strategies taken to produce natural products are reviewed. For convenience, natural products are classified into four main categories: terpenoids, phenylpropanoids, polyketides, and alkaloids. For each product category, the strategies for establishing and rewiring the metabolic network for heterologous natural product biosynthesis, systems approaches undertaken to optimize production hosts, and the strategies for fermentation optimization are reviewed. Taken together, metabolic engineering has enabled microorganisms to serve as a prominent platform for natural compounds production. This article examines both the conventional and novel strategies of metabolic engineering, providing general strategies for complex natural compound production through the development of robust microbial‐cell factories.

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Section: Optimization Of Fermentation Conditionmentioning

confidence: 99%

Metabolic Engineering of Microorganisms for the Production of Natural Compounds

Park

Yang

et al. 2017

Adv. Biosys.

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“…A recombinant E. coli strain overexpressing the genes crtE, crtB, and crtI from Deinococcus radiodurans R12 and optimizing the Shine Dalgarno regions and aligned spacing sequence could produce 88 mg/g lycopene after 40 h fermentation [28]. E. coli K12f could efficiently uptake fructose; overexpressing the crtEBI operon from Pantoea ananatis.…”

Section: Discussionmentioning

confidence: 99%

Effects of lipopolysaccharide structure on lycopene production in Escherichia coli

Cui

Zhou

et al. 2019

Enzyme and Microbial Technology

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A B S T R A C TLipopolysaccharides, the major molecules in the outer membrane of Escherichia coli, affect the behavior of bacteria including outer membrane permeability, but its influence on lycopene production in E. coli has never been reported. In this study, the effects of lipopolysaccharides with different structures on lycopene biosynthesis were investigated. Firstly, the heterogenous crtEBI operon were overexpressed in 10 LPS mutant strains of E. coli W3110 (ΔwaaC, ΔwaaF, ΔwaaY, ΔwaaG, ΔwaaR, ΔwaaO, ΔwaaU, ΔwaaP, ΔwaaY and ΔwaaB), and their ability to produce lycopene were compared. ΔwaaC/pWSK29-crtEBI, ΔwaaF/pWSK29-crtEBI and ΔwaaY/pWSK29-crtEBI produced 4.19, 4.20, and 3.81 mg/g lycopene, respectively, while the control W3110/pWSK29-crtEBI produced 3.71 mg/g lycopene; the other strains produced less lycopene than the control. In order to enhance lycopene production, genes dxr, dxr, ispA, and idi were overexpressed in ΔwaaC/pWSK29-crtEBI, ΔwaaF/pWSK29-crtEBI individually or in combination, and the lycopene production in each strain was analyzed. The maximum yield of 5.39 mg/g was achieved in ΔwaaC/pWSK29-crtEBI-SRA, which is 142% higher than that in W3110/pWSK29-crtEBI. The results indicate that the length of lipopolysaccharide affects lycopene biosynthesis in E. coli, and the shorter lipopolysaccharide and higher outer membrane permeability might be beneficial to lycopene biosynthesis.

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“…Directed evolution has been applied to geranylgeranyl diphosphate GGPP synthase from Archaeoglobus fulgidus being the rate-controlling enzyme to enhance the production of carotenoids in metabolically engineered Escherichia coli. The library of mutated genes that has been created using these tools was transformed into an E. coli strain containing the reconstructed isoprenoid pathway (Jin et al, 2015), and screened for color intensity to optimize the conversion of glucose into astaxanthin or lycopene. In summary, from more than 10,000 colonies, 8 mutants were obtained.…”

Section: Directed Evolution and Molecular Breeding Techniques For Thementioning

confidence: 99%

Microbial Pigments From Bacteria, Yeasts, Fungi, and Microalgae for the Food and Feed Industries

Dufossé

2018

Natural and Artificial Flavoring Agents and Food Dyes

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Putative carotenoid genes expressed under the regulation of Shine–Dalgarno regions in Escherichia coli for efficient lycopene production

Abstract: Based on the optimization of the upstream SD regions and culture medium, the yield of the strain EDW11 reached a high level during microbial lycopene production until now.

Cited by 23 publications

References 22 publications

Metabolic Engineering of Microorganisms for the Production of Natural Compounds

Metabolic Engineering of Microorganisms for the Production of Natural Compounds

Effects of lipopolysaccharide structure on lycopene production in Escherichia coli

Microbial Pigments From Bacteria, Yeasts, Fungi, and Microalgae for the Food and Feed Industries

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