Putative Primo-Vascular System in Rabbit Placenta

Mustafa, Fatma El‐Zahraa A.

doi:10.1016/j.jams.2019.01.001

Cited by 17 publications

(21 citation statements)

References 12 publications

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“…Negative images were performed using CMEIAS Color Segmentation, an improved computing technology used to process color images by segmenting the foreground object of interest from the background 64 . This has been done by the following steps: open the image with CMEIAS Color Segmentation, then select "Process" from the menu items, and subsequently choose "Negative image" [68][69][70][71] .…”

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confidence: 99%

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Abd‐Elhafeez

Abou-Elhamd

Soliman

2020

Sci Rep

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the current study investigated telocytes (tcs) in the intestinal bulb of Grass carp using light microscopy (LM), transmission electron microscopy (teM), scanning electron microscopy, and immunohistochemistry (iHc). By LM, tcs were distinguished by the typical morphological features that had a cell body and telopodes using He, toluidine blue, methylene blue, Marsland silver stain, Grimelius's silver nitrate, Giemsa, PAS, combined AB pH2,5/PAS, Crossmon's and Mallory triple trichrome, Van Gieson stains, Verhoeff's stain, Sudan black, osmic acid, performic acid with methylene blue and bromophenol blue. TCs were identified under the epithelium as an individual cell or formed a TCs sheath. They detected in the lamina propria, between muscle fibers, around the myenteric plexus and fibrous tissue. TCs acquired immunological features of endocrine cells that exhibited high affinity for silver stain, performic acid with methylene blue, Marsland stain, and immunohistochemical staining using chromogranin A. Sub epithelial tcs were closely related to the endocrine cells. tcs and their secretory activities were recognized using acridine orange. TCs were identified by IHC using CD34, CD117, S100-protein, desmin. TCs formed a3D network that established contact with macrophage, mast cells, dendritic cells, lymphocytes, smooth muscle fibers, fibroblast, Schwann cells and nerve fibers. In conclusion, the localization of TCs in relation to different types of immune cells indicated their potential role in the maintenance of intestinal immunity. Interstitial cells have critical roles in the maintenance of the appropriate 3D scaffold and functional requirements of the organs. Telocytes (TCs) are the cell population that forms a network through a labyrinthine system formed by telopodes. They are long and slender prolongations reached up to hundreds of microns. Telopodes are identified by their segments; the thin segment, podomer and the thick segment, the podoms. TCs established contact to other resident cells or to wandering cells via their telopodes or via the cell body. Telopodes network provide-long-distance cell-cell signaling (intercellular communication) 1. Paracrine signaling is critical for TCS function. TCs produce several secretory molecules and factors contributing to the functional significance.Gene analysis 2 and proteomics analysis 3 are investigated for TCs. The functional contributions of TCS are supposed to be related to angiogenesis 4 , and development, maintenance of homeostatic balance 4 , immunosurveillance 5 , tissue regeneration and repair through providing adequate microenvironment for stem cell niche and promoting their differentiation 6,7. TCs are identified in a wide variety of organs from diverse species including mammals 8-11 , avian 12,13 , reptiles 14 , Amphibians 15 and aquatic species 16,17 and parasitic worm 18. They are located in the trachea and lungs 19 , heart 20 and the blood vessels 21 , kidney, ureter, urinary bladder 22 , tongue 23 , oesophagus, stomach and small and large

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confidence: 99%

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Abd‐Elhafeez

Abou-Elhamd

Soliman

2020

Sci Rep

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“…Negative images were obtained using the CMEIAS color segmentation software, which processes color images by segmenting the object of interest in the foreground from the background 52 . This process is conducted by the following steps: open image file with CMEIAS color segmentation, then select “Process” from the menu items and subsequently choose “Negative image” 53 – 55 .…”

Section: Methodsmentioning

confidence: 99%

Melatonin administration provokes the activity of dendritic reticular cells in the seminal vesicle of Soay ram during the non-breeding season

Abd‐Elhafeez

Hassan

Hussein

2021

Sci Rep

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Dendritic cells (DCs) are innate immune cells which engulf, process and present antigens to the naïve T-lymphocyte cells. However, little is known about the effect of melatonin on the DCs. The present study aimed to investigate the morphology and distribution of the DCs by transmission electron microscopy and Immunohistochemistry after melatonin administration. A total of 8 out of 15 adult ram was randomly selected to receive the melatonin implant and the remaining 7 animals received melatonin free implants. DCs showed positive immunoreactivity for CD117, S-100 protein and CD34. There is an obvious increase in the number of the positive immunoreactive cells to CD3, estrogen receptor alpha and progesterone in the treated groups. The expression of CD56 and MHCII in the DCs was abundant in the treated groups. The ultrastructure study revealed that melatonin exerts a stimulatory effect on the DCs which was associated with increment in the secretory activity of DCs. The secretory activity demarcated by an obvious increase in the number of mitochondria, cisternae of rER and a well-developed Golgi apparatus. The endosomal- lysosomal system was more developed in the treated groups. A rod-shaped Birbeck granule was demonstrated in the cytoplasm of the melatonin treated group. DCs were observed in a close contact to telocytes, T-Lymphocytes, nerve fibers and blood vessels. Taken together, melatonin administration elicits a stimulatory action on the DCs and macrophages through increasing the size, the number and the endosomal compartments which may correlate to increased immunity.

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“…Negative images were obtained using the CMEIAS Color Segmentation, an improved computer program that can process color images by segmenting the foreground objects of interest from the background. Each negative image was obtained through the following steps: the image was opened with CMEIAS Color Segmentation, “Process” was selected from the menu, and “Negative image” was chosen (Gross et al, 2010; Abd-Elkareem, 2017; Abdel-Maksoud et al, 2019; Mustafa, 2019; Soliman et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

Morphological and Histochemical Characterization of the Dermal Plates of Pleco (Hypostomus plecostomus)

et al. 2020

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Studying the dermal skeleton in fish is valuable for phylogenetic specification. The current study describes the detailed structure of the plecostomus dermal skeleton, including its morphogenesis and distribution in the skin. The denticles have a crown and a basal part and are embedded in bony depressions, to which they are attached by denticle ligaments. During denticle morphogenesis, denticle papillae formed from denticle precursor cells align in two cellular layers: an outer ameloblast precursor layer and an inner odontoblast precursor layer. The ameloblast precursors and odontoblast precursors differentiate and secrete enamel and dentine, respectively. We used different histochemical techniques, including Crossmon's trichrome staining, Weigert–Van Gieson staining, periodic acid–Schiff (PAS) staining, combined Alcian blue (AB; pH 2.5)/PAS staining, Weigert–Van Gieson staining, Mallory trichrome staining, and AB staining to distinguish the dentine and denticle ligaments. We used acridine orange to detect lysosome activity during denticle eruption. Transmission electron microscopy was used to detect the denticle ultrastructure, and scanning electron microscopy was used to detect the topographic distributions of different types of dermal tissues in different anatomical regions.

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Putative Primo-Vascular System in Rabbit Placenta

Cited by 17 publications

References 12 publications

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Melatonin administration provokes the activity of dendritic reticular cells in the seminal vesicle of Soay ram during the non-breeding season

Morphological and Histochemical Characterization of the Dermal Plates of Pleco (Hypostomus plecostomus)

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