Putting Opposites Guidelines for Successful SFC/MS

Abstract

Guidelines for Successful SFC/MS n the early days of on-line LC/MS, Arpino likened the union of these two instruments to the unlikely marriage of a bird and a fish: "Many believe this coupling is even more difficult to achieve than the love-match ... between two creatures that are at ease in their own environments but are not at home in both" (1). At first consideration, this combination of high-pressure and vacuum techniques does seem preposterous. Removing a molecule from its high-pressure solvent, transporting it preferentially over vaporized solvent to a partial vacuum, and imparting a charge on the analyte does seem a challenging feat. However, great progress was made and LC/MS applications have become routine.But what if the analyte were dissolved in a fluid that, at high pressure, solvated like a traditional liquid but transformed to an easily removed gas when the pressure dropped, leaving the analyte free to "fly like a bird"? This was the promise offered by supercritical fluid chromatography (SFC)/MS when research in the area began in the late 1960s (2-5). Admittedly, the analogy should not be carried too far. Nevertheless, the commonly used mobile phases in SFC/MS, such as C02, are much more easily pumped from a high to moderate vacuum system than are

Flow Injection Analysis with Turbidity Detection for the Quantitative Determination of Mebeverine Hydrochloride in Pharmaceutical Formulations

“…In the literature, several analytical procedures have been described for the quantitative determination of MbH in the pharmaceutical formulations, bulk and biological fluids. Among the described methods are electrochemical 9 , spectrofluorometric [10][11] , spectrophotometric [12][13][14][15][16][17][18][19] , chromatographic method 20, 21 , MIP 22 , HPLC [23][24][25][26][27][28][29] , potentiometric 30,31 , online micellar chromatography 32 , HPTLC 33 , super critical fluid GC-MS 34 , and reversed-phase LC-GC 35 . Mainly the chromatographic methods in spite of their high sensitivity and accuracy but are timeconsuming, expensive and require special laboratory training.…”