It is known that the data acquisition and processing system plays an important role in radar target detection system. In order to meet the requirements of real-time processing and accurate transmission of echo signals in high-frequency ground-wave radar (HFGWR) systems, a new acquisition and transmission framework utilizing the designed acquisition card based on the PCIe (peripheral component interconnect express) has been designed and is presented in this paper. The Xilinx FPGA (Field-Programmable Gate Array) chip Kintex7-XC7K325T is adopted as a hardware carrier in acquisition card. The hardware’s composition, analog front-end circuit, the DMA (Direct Memory Access) transmission, FPGA structure, ADC (Analog-to-Digital Converter) chip, and performance test of this card are showed and discussed. Currently, the acquisition card has been accomplished and applied in the practical system of HFGWR.