Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereus

Doshi, Minoli Nitin; Nair, Kavita; Hassan, Zahoor Ul; Jaoua, Samir

doi:10.1016/j.biteb.2022.101106

Cited by 5 publications

(7 citation statements)

References 44 publications

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“…Simultaneously, the antibacterial activity of CFSs from the isolates against indicator strains ( S. epidermis ) was evaluated using the AWD method at the same time points. This allowed us to pinpoint the onset and progression of antibacterial metabolite production over time [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

“…Bacteriocins play a crucial role in defensive and competitive mechanisms. These extracellular toxins, produced by ribosomes, work by inhibiting or killing rival strains (those close to the main microorganism), ultimately enhancing survival by securing biological living space and improving access to nutrients [ 24 – 28 ].…”

Section: Introductionmentioning

confidence: 99%

“…This opportunistic Gram-negative gammaproteobacterium is capable of producing an array of extracellular products which ultimately serve to facilitate its spread [ 29 ]. Since 90% of P. aeruginosa strains are capable of producing at least one type of bacteriocins, they are an excellent model for protein based antibiotic investigation [ 28 ]. Bacteriocins produced by P. aeruginosa capable of inhibiting other strains are called pyocins, which are classified into three groups: S, R, and F. S-pyocins are large polypeptides with multiple domains.…”

Section: Introductionmentioning

confidence: 99%

“…Bacteriocins produced by P. aeruginosa capable of inhibiting other strains are called pyocins, which are classified into three groups: S, R, and F. S-pyocins are large polypeptides with multiple domains. They include a cognate immunity protein that deactivates the catalytic domain of the active pyocin [ 28 – 31 ]. S-pyocins are soluble and sensitive to heat and proteases, a trait that distinguishes them from R- and F-pyocins [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

“…S-pyocins are soluble and sensitive to heat and proteases, a trait that distinguishes them from R- and F-pyocins [ 29 , 30 ]. R- and F-type pyocins, also known as tailocins, originated as defective prophages with ancestral connections to P2 and lambda phages [ 28 – 31 ]. R-type pyocins possess a contractile structure but lack flexibility, whereas F-type pyocins are flexible but lack contractility [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of bacteriocin production by clinical Pseudomonas aeruginosa isolates and their potential as therapeutic agents

Charkhian,

Soleimannezhadbari,

Bodaqlouei

et al. 2024

Microb Cell Fact

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Introduction Bacterial infections and the rising antimicrobial resistance pose a significant threat to public health. Pseudomonas aeruginosa produces bacteriocins like pyocins, especially S-type pyocins, which are promising for biological applications. This research focuses on clinical P. aeruginosa isolates to assess their bacteriocin production, inhibitory spectrum, chemical structure, antibacterial agents, and preservative potential. Methods The identification of P. aeruginosa was conducted through both phenotypic and molecular approaches. The inhibitory spectrum and antibacterial potential of the isolates were assessed. The kinetics of antibacterial peptide production were investigated, and the activity of bacteriocin was quantified in arbitrary units (AU ml−1). Physico-chemical characterization of the antibacterial peptides was performed. Molecular weight estimation was carried out using SDS–PAGE. qRT-PCR analysis was employed to validate the expression of the selected candidate gene. Result The antibacterial activity of P. aeruginosa was attributed to the secretion of bacteriocin compounds, which belong to the S-type pyocin family. The use of mitomycin C led to a significant 65.74% increase in pyocin production by these isolates. These S-type pyocins exhibited the ability to inhibit the growth of both Gram-negative (P. mirabilis and P. vulgaris) and Gram-positive (S. aureus, S. epidermidis, E. hirae, S. pyogenes, and S. mutans) bacteria. The molecular weight of S-type pyocin was 66 kDa, and its gene expression was confirmed through qRT-PCR. Conclusion These findings suggest that S-type pyocin hold significant potential as therapeutic agents against pathogenic strains. The Physico-chemical resistance of S-type pyocin underscores its potential for broad applications in the pharmaceutical, hygiene, and food industries.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Assessment of bacteriocin production by clinical Pseudomonas aeruginosa isolates and their potential as therapeutic agents

Charkhian,

Soleimannezhadbari,

Bodaqlouei

et al. 2024

Microb Cell Fact

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Bacteriocins: potentials and prospects in health and agrifood systems

Reuben,

Torres

2024

Arch Microbiol

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Bacteriocins are highly diverse, abundant, and heterogeneous antimicrobial peptides that are ribosomally synthesized by bacteria and archaea. Since their discovery about a century ago, there has been a growing interest in bacteriocin research and applications. This is mainly due to their high antimicrobial properties, narrow or broad spectrum of activity, specificity, low cytotoxicity, and stability. Though initially used to improve food quality and safety, bacteriocins are now globally exploited for innovative applications in human, animal, and food systems as sustainable alternatives to antibiotics. Bacteriocins have the potential to beneficially modulate microbiota, providing viable microbiome-based solutions for the treatment, management, and non-invasive bio-diagnosis of infectious and non-infectious diseases. The use of bacteriocins holds great promise in the modulation of food microbiomes, antimicrobial food packaging, bio-sanitizers and antibiofilm, pre/post-harvest biocontrol, functional food, growth promotion, and sustainable aquaculture. This can undoubtedly improve food security, safety, and quality globally. This review highlights the current trends in bacteriocin research, especially the increasing research outputs and funding, which we believe may proportionate the soaring global interest in bacteriocins. The use of cutting-edge technologies, such as bioengineering, can further enhance the exploitation of bacteriocins for innovative applications in human, animal, and food systems.

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Production and SERS characterization of bacteriocin-like inhibitory substances by latilactobacillus sakei in whey permeate powder: exploring natural antibacterial potential

Contessa,

Moreira,

Moraes

et al. 2024

Bioprocess Biosyst Eng

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Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereus

Cited by 5 publications

References 44 publications

Assessment of bacteriocin production by clinical Pseudomonas aeruginosa isolates and their potential as therapeutic agents

Assessment of bacteriocin production by clinical Pseudomonas aeruginosa isolates and their potential as therapeutic agents

Bacteriocins: potentials and prospects in health and agrifood systems

Production and SERS characterization of bacteriocin-like inhibitory substances by latilactobacillus sakei in whey permeate powder: exploring natural antibacterial potential

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