Pyramided rice lines harbouring Allium sativum (asal) and Galanthus nivalis (gna) lectin genes impart enhanced resistance against major sap-sucking pests

Bharathi, Y.; Kumar, Shiv; Pasalu, I. C.; Balachandran, S. M.; Reddy, V. D.; Rao, K. V.

doi:10.1016/j.jbiotec.2011.01.021

Cited by 35 publications

(19 citation statements)

References 31 publications

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“…1, Table 1). Transgenic tobacco expressing garlic lectin offers resistance against Spodoptera litura [77] and Myzus persicae [23], transgenic rice against brown plant hopper, green leafhopper and white backed plant hopper [10,105] and transgenic chickpea against Aphis craccivora [14].…”

Section: Garlic Lectins As Bio-pesticidesmentioning

confidence: 99%

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Receptors of Garlic (Allium sativum) Lectins and Their Role in Insecticidal Action

Upadhyay

Singh

2012

Protein J

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Garlic (Allium sativum) lectins are promising candidate molecules for the protection against chewing (lepidopteran) as well as sap sucking (homopteran) insect pests. Molecular mechanism of toxicity and interaction of lectins with midgut receptor proteins has been described in many reports. Lectins show its effect right from sensory receptors of mouth parts by disrupting the membrane integrity and food detection ability. Subsequently, enter into the gut lumen and interact with midgut glycosylated proteins like alkaline phosphatase (ALP), aminopeptidase-N (APN), cadherin-like proteins, polycalins, sucrase, symbionin and others. These proteins play critical role in life cycle of insect directly or indirectly. Lectins interfere with the activity of these proteins and causes physiological disorders leading to the death of insects. Lectins further transported across the insect gut, accumulated in various body parts (like haemolymph and ovary) and interact with intracellular proteins like symbionin and cytochrome p450. Binding with cytochrome p450 (which involve in ecdysone synthesis) might interfere in the development of insects, which results in growth retardation and pre-mature death.

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Section: Garlic Lectins As Bio-pesticidesmentioning

confidence: 99%

“…These are glycosylated proteins and usually anchored on membranes by single trans-membrane domain. But cadherins with seven-trans-membrane Transgenic rice [10,105] Lypaphis erysimi (Aphid)…”

Section: Cadherin Like Proteinmentioning

confidence: 99%

Receptors of Garlic (Allium sativum) Lectins and Their Role in Insecticidal Action

Upadhyay

Singh

2012

Protein J

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“…Garlic lectins remained the focus of various transformation studies. Studies such as pyramided rice lines (Foissac et al, 2000;Nagadhara et al, 2003); marker genefree transgenic tobacco (Bharathi et al, 2011); chickpea (Chakraborti et al, 2009); rice (Yarasi et al, 2008) show effectiveness of lectins against sucking insect population. The efficacy of snowdrop (G. nivalis) and garlic lectins have been studied against phloem feeding Aphis craccivora, cotton aphids A. gossypii (Sauvion et al, 1996), red spider mite of tea (Roy et al, 2008), cotton leafworm and sapsucking planthoppers (Powell, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Transformation studies have also been conducted to study efficacy of lectins against sucking insects in pyramided rice lines (Bharathi et al, 2011), marker gene-free transgenic rice (Yarasi et al, 2008;Sengupta et al, 2010), tobacco (Sadeghi et al, 2008) and chickpea (Chakraborti et al, 2009). However, studies have not been conducted on the gene expression levels of lectin and roles of its protein structure in insecticidal activity.…”

Section: Introductionmentioning

confidence: 99%

Computational and Real-Time RT-PCR Expression Analyses of Garlic Lectin Gene

Usman¹,

Rehman²,

Khan³

et al. 2015

IJAB

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Sucking insect pests inflict heavy losses to important crops. Bt gene being unable to control sucking insects, lectin seems to be a good alternative insecticidal gene. However, studies on sequence analysis, evolutionary relationship and expression patterns of garlic lectin genes remained elusive. Therefore, this study was envisaged to explore lectin gene sequence diversity in different species, and to analyze differential expression of lectin genes in garlic bulb, leaf and root tissues. Phylogenetic reconstruction did not reveal well-defined clusters indicating divergence in lectin sequences in different plant species. Multiple alignments exhibited conservation in lectin coding sequences in the middle regions. On the other hand, both the N-and Ctermini were quite divergent and size of the coding regions varied greatly in different orthologs probably due to exonization of introns. Semi-quantitative and quantitative RT-PCR exhibited elevated expression of lectin genes in garlic bulb as compared to leaf and root tissues. this study has future implications in transforming insecticidal genes in crops against sucking insects.

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“…Because of the potent entomotoxic properties exhibited by GNA, the GNA gene has been introduced into a number of plants, including tobacco [11], potato [1,6], tomato [33], wheat [18,28] and maize [34]. GNA has also been introduced into rice [2,5,20,23,27,29,30], and transgenic rice plants containing this gene show increased resistance to BPH in bioassays and feeding tests [5,23,29]. This gene has negligible effects on the development, survival, and fecundity of beneficial insects [4].…”

Section: Introductionmentioning

confidence: 99%

Transgenic Rice Expressing Snowdrop Lectin (Galanthus nivalis agglutinin; GNA) Shows Resistance to Rice Brown Planthopper (Nilaparvata lugens Stål)

Lee¹,

Yoon²,

Hong³

et al. 2012

Journal of Life Science

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Transgenic rice plants with increased resistance to rice brown planthopper (Nilaparvata lugens Stål) were generated by particle bombardment-mediated transformation of plants with a gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under control of the rice Rubisco small subunit (rbcS) promoter.. A large number of transgenic rice plants containing the GNA gene were generated.The integration, expression, and inheritance of this gene in the R1 and R2 generations were demonstrated by Southern and western blot analyses. The plants contained one to five copies of the transgene. The GNA protein comprised approximately 0.01-2.0% of total soluble protein in the R1 and R2 transgenic plants. Insect bioassays and feeding studies showed that the GNA protein expressed in the R2 transgenic rice plants reduced the survival of brown planthoppers. The introduction of GNA into rice plants therefore can help to control insect pests.

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Pyramided rice lines harbouring Allium sativum (asal) and Galanthus nivalis (gna) lectin genes impart enhanced resistance against major sap-sucking pests

Cited by 35 publications

References 31 publications

Receptors of Garlic (Allium sativum) Lectins and Their Role in Insecticidal Action

Receptors of Garlic (Allium sativum) Lectins and Their Role in Insecticidal Action

Computational and Real-Time RT-PCR Expression Analyses of Garlic Lectin Gene

Transgenic Rice Expressing Snowdrop Lectin (Galanthus nivalis agglutinin; GNA) Shows Resistance to Rice Brown Planthopper (Nilaparvata lugens Stål)

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