Pyrene‐Excimer Probes Based on the Hybridization Chain Reaction for the Detection of Nucleic Acids in Complex Biological Fluids

Abstract

China Scholarship Council (CSC); ACS; US NIH; China NSFC[20805038]; National Basic Research Program of China[2007CB935603, 2010CB732402]; China National Grand Program on Key Infectious Disease[2009ZX10004-312]; Key Project of Natural Science Foundation of China[90606003]; International Science & Technology Cooperation Program of China[2010DFB30300]; Hunan Provincial Natural Science Foundation of China[10JJ7002

Amplified detection of nucleic acid by G-quadruplex based hybridization chain reaction

“…Most of these works concentrated on the signal reporters, to make HCR a more applicable amplification method: e.g. nano-gold particle (Pierce et al, 2006), pyrene molecule (Huang et al, 2011), fluorophore/quencher (FAM/Dabsyl; Chemeris et al, 2008), and biotin (Niu et al, 2010) modified reporter probes.…”

Signal Amplification for Highly Sensitive Immunosensing

“…With great attention focused on enzyme-free isothermal amplification strategies, hybridization chain reaction (HCR) amplification shows great potential in signal amplification [71][72][73]. In HCR, two sets of DNA monomer hairpin structure (H1 and H2) are designed to be partially complementary, which provides a building blocks via amplifying short sequences of oligonucleotides, and achieves an enzyme-free alternative for selective and specific extension at room temperature.…”

DNA-based advanced logic circuits for nonarithmetic information processing

“…Considering the four resultant signals, it is a critical issue to select dye molecules that attach to DNA strands and can generate different readout signals in the emission-only mode under different conditions. To eliminate spectra overlap and secondary excitation, four different fluorescent groups are selected, including FAM, NMM, HEX and a pyrene-based excimer 34 with excitation/emission at 485/517 nm, 399/608 nm, 535/555 nm and 340/488 nm, respectively. The fluorescence of FAM, NMM, HEX and the excimer is defined as Output 0, Output 1, Output 2 and Output 3, respectively.…”