Pyrolysis based bio-refinery for the production of bioethanol from demineralized ligno-cellulosic biomass

Microbial conversion of pyrolytic products to biofuels: a novel and sustainable approach toward second-generation biofuels

“…Wang et al [187] used non-polar hexane and polar linoleic acid to remove the inhibitors from bio-oil hydrolysate, and their method was moderately successful. In more recent study, Luque et al [106] used a 1:2 wt.% of bio-oil filtrate and ethyl acetate to remove the yeast growth inhibitors e.g., phenolic compounds, furans, and aldehydes obtaining 96 % of the theoretical yield in microtiter plate wells. According to Luque et al [106], extraction with ethyl acetate is relatively selective, largely removes the other organic compounds, and there is a no significant loss of levoglucosan.…”

“…In more recent study, Luque et al [106] used a 1:2 wt.% of bio-oil filtrate and ethyl acetate to remove the yeast growth inhibitors e.g., phenolic compounds, furans, and aldehydes obtaining 96 % of the theoretical yield in microtiter plate wells. According to Luque et al [106], extraction with ethyl acetate is relatively selective, largely removes the other organic compounds, and there is a no significant loss of levoglucosan. In another study, Hassan et al [61] compared the efficiency of n-butanol and ethyl acetate on removing phenolic compounds and other enzymatic inhibitors from aqueous fraction bio-oil and they found that n-butanol solvent was more effective than ethyl acetate in removing such compounds.…”

Lipid accumulation from pinewood pyrolysates by Rhodosporidium diobovatum and Chlorella vulgaris for biodiesel production

“…vanillin and cresols) (Saha et al, 2005). These inhibitory compounds can be carried over from pyrolysis through the upgrading steps and can later affect growth and production formation at sufficient concentrations (Garcia-perez et al, 2008a,b;Luque et al, 2014;Wood et al, 2015). It was previously shown that the upgrading process shown in Fig.…”

“…It was previously shown that the upgrading process shown in Fig. 1 can decrease the abundance of inhibitory compounds below their inhibitory concentration allowing unimpeded cultivation of Saccharomyces cerevisiae for ethanol fermentation (Luque et al, 2014).…”

“…Pyrolysis is the thermal decomposition of biomass at temperatures typically around 500°C in the absence of oxygen. Under these conditions, a carbohydrate-rich liquid stream composed primarily of levoglucosan (1,6-anhydro-b-glucopyranose) can be recovered through factional condensation of the condensable gases or 'bio-oil', which can be used after hydrolysis and upgrading as a feedstock for several bioconversion processes generating additional value for the pyrolysis process (Luque et al, 2014).…”