Pyrolytic profiling nitrosamine specific chemiluminescence detection combined with multivariate chemometric discrimination for non-targeted detection and classification of nitroso compounds in complex samples

Vrzal, Tomáš; Olšovská, Jana

doi:10.1016/j.aca.2019.01.033

Cited by 9 publications

(18 citation statements)

References 18 publications

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“…The mass spectrum of peak 7 may be assigned to a previously published spectrum of the unknown nitroso compound (Figure S3a−c). 15 The MS spectrum of this peak showed similar fragmentation to NPRO−TMS ester, with a difference that this peak's fragments were higher by 14 m/z units. Hence, it was affiliated to N-nitrosopyroglutamic acid− TMS (NPGA−TMS) or any isomer of N-nitrosopipecolic acid−TMS (NPIC−TMS) as standard NPIC−TMS nearly did not match in retention on the TG-200MS column.…”

Section: ■ Results and Discussionmentioning

confidence: 83%

“…It should be noted that this substance was also detected in GC− MS chromatograms performed on samples during a previous study, where an artificially contaminated beer sample by Obesumbacterium proteus (a known microbial agent responsible for the production of nitroso compounds in beer) 9 was analyzed. 15 Mass spectra of peak 3 (Figure S5a,b) showed the highest match with 4-cyanophenol−TMS (98.3%, MW 191). Although the comparison of RI exp matched RI pred , it did not match RI lib (Table 1).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Hence, it was affiliated to N-nitrosopyroglutamic acid− TMS (NPGA−TMS) or any isomer of N-nitrosopipecolic acid−TMS (NPIC−TMS) as standard NPIC−TMS nearly did not match in retention on the TG-200MS column. 15 For this reason, the RI exp and RI pred for all possible structures were compared and showed the best match with NPIC−TMS and NPGA−TMS. Therefore, the other isomers were due to RI comparison denied (Figure S3d).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Another important finding was structural characterization of unknown compounds based on MS/MS fragmentation experiments, which led to the identification of two most significant peaks as N-nitrosoproline (NPRO) and its ethyl ester (NPRO−Et). 15 However, except for N-nitrosoproline, its ethyl ester, and two more unknown nitroso compounds, the other compounds of interest remained unidentified.…”

Section: ■ Introductionmentioning

confidence: 99%

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Characterization of Nitrite-Related Reaction Products in Beer

Malečková

Vrzal

Olšovská

et al. 2021

J. Agric. Food Chem.

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The effect of nitrites in foods and beverages still raises discussion due to the possible formation of harmful nitroso compounds. However, as most of these compounds in beer were not structurally characterized yet, the research about their toxicological relevance for consumers is limited. This study is focused on identification of the products formed by nitrite (or isotopically labeled nitrite 15N) reactions in beer using gas chromatography with tandem mass spectrometry. In total, 19 products were identified, and some of them were structurally characterized and confirmed by comparing retention indices and mass spectra of standard/synthesized compounds. Identified compounds were representatives of nitroso, nitro, oxime, and even cyano compounds. For the peaks which were not structurally identified, primary structural characteristics were also listed. Found products were further screened in 16 authentic beer samples which showed the apparent occurrence of found compounds in non-treated beers.

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Section: ■ Results and Discussionmentioning

confidence: 83%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Nitrite-Related Reaction Products in Beer

Malečková

Vrzal

Olšovská

et al. 2021

J. Agric. Food Chem.

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“…They are therefore determined together with volatile compounds as the apparent sum of all N-nitroso compounds, called ATNC (Fan and Lin, 2018;Lachenmeier and Fügel 2007). However, a new study on the determination of non-volatile N-nitrosamines developed at the RIBM has been published recently (Vrzal and Olšovská, 2019).…”

Section: Nitrosamines and Atncmentioning

confidence: 99%

Monitoring of potential contaminants in beer from the Czech Republic

Olšovská

Jandovská

Běláková

et al. 2019

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The health safety of beer is still a discussed topic. In the Czech Republic, there is a well-developed system for controlling the content of potential beer contaminants, which are regularly attended by a number of large and medium-sized breweries and are organized by laboratories of the Research Institute of Brewing and Malting. For many years aliphatic chlorinated hydrocarbons, biogenic amines, nitrates, phthalates, ATNC, nitrosamines, polychlorinated biphenyls, polycyclic aromatic hydrocarbons, heavy metals, mycotoxins, coliforms, and recently pesticides in beer have been analyzed. This study summarizes five-year results and extends information about the origin, health risks and hygiene limits of controlled substances and bacteria in beer. The study proves that the level of health safety of beers in the Czech Republic is very high, which is closely related to the high hygiene level of controlled breweries. The number of positive samples was only minor, 8 positive samples were found in 220 beer samples controlled for 44 chemical contaminants. Three positive samples contaminated by coliform bacteria were found among 675 controlled ones.

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