Pyrosequencing Analysis of Cryogenically Ground Samples from Primary and Secondary/Persistent Endodontic Infections

Keskin, Cangül; Demiryürek, Ebru Özsezer; Onuk, Ertan Emek

doi:10.1016/j.joen.2017.03.019

Cited by 68 publications

(81 citation statements)

References 36 publications

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“…In these cases, the teeth investigated were to be retained in the oral cavity. Seven studies (7/18) employed an ex vivo sampling methodology, in which the roots were extracted prior to sampling the microbiota . Six of these studies relied on cryo‐pulverization of root sections, a technique used in sectioning the apical extremity of the root, that is then ground at liquid nitrogen temperatures in dedicated freezer mills .…”

Section: Resultsmentioning

confidence: 99%

“…PAP and SAP samples were compared in five studies . Among these, three studies reported differentially abundant microbiotas between PAPs and SAPs .…”

Section: Resultsmentioning

confidence: 99%

“…and Keskin et al. found no significant differences between PAP and SAP pathologies neither in terms of community composition, nor in terms of diversity …”

Section: Resultsmentioning

confidence: 99%

“…In addition to NECs, negative controls serve to assess the contamination background specific to the sampling site . Herein, 12 of 18 studies have included negative controls mainly taken from endodontic access cavities or external root surfaces after disinfection, to verify the relative “sterility” of the site prior to sampling . Among these 12 studies, four used vital teeth as negative controls, which could also be regarded as biological/healthy controls .…”

Section: Discussionmentioning

confidence: 99%

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A Systematic Review of the Root Canal Microbiota Associated with Apical Periodontitis: Lessons from Next‐Generation Sequencing

Manoil

Al‐Manei

Belibasakis

2020

Proteomics Clinical Apps

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Next-generation sequencing (NGS) has now been applied for a decade to characterize the microbiota composition of infected dental root canals associated with apical periodontitis. Here, the study aims at systematically and critically reviewing these reports within the outcome of interest selected; the microbiota composition in different endodontic infection types. Standard methodological guidelines as stated by the PRISMA and the Joanna Briggs Institute are followed, including a risk of bias assessment. A literature search is conducted using the PubMed Advanced-Search Builder on April 8, 2019; only original research articles that investigated the microbiota of infected root-canals by means of NGS are screened. Among the 26 articles initially identified, 18 are included and evaluated for the following parameters; sampling protocol, sequencing strategy, and microbiota composition. The endodontic infections include primary apical periodontitis (PAP), secondary apical periodontitis (SAP), and apical abscess (AA). All infection types are associated with a highly diverse microbiota. Although some taxa appear differentially abundant between PAPs, SAPs, and AAs, no evident clustering of the microbiota by infection type is observed. These studies collectively formulate a comprehensive map of the taxa associated with endodontic infections and provide evidence of compositionally unspecific, yet abundance differentiates, community profiles according to clinical diagnosis.The ORCID identification number(s) for the author(s) of this article can be found under https://doi.

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Section: Resultsmentioning

confidence: 99%

“…PAP and SAP samples were compared in five studies . Among these, three studies reported differentially abundant microbiotas between PAPs and SAPs .…”

Section: Resultsmentioning

confidence: 99%

“…and Keskin et al. found no significant differences between PAP and SAP pathologies neither in terms of community composition, nor in terms of diversity …”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

A Systematic Review of the Root Canal Microbiota Associated with Apical Periodontitis: Lessons from Next‐Generation Sequencing

Manoil

Al‐Manei

Belibasakis

2020

Proteomics Clinical Apps

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show abstract

“…Some of them might be also implicated in the onset of pulp inflammation and its transition to irreversible acute or chronic pulpitis (11,12). However, commonly the microflora of deep caries is completely different compared to dominant microrganisms genera found in infected root canals, such as Porphyromonas, Prevotella, Peptostreptococcus, Fusobacterium, and others (13,14).…”

Section: Deep Caries Microbiomementioning

confidence: 99%

Molecular Mechanisms of Dentine-Pulp Complex Response Induced by Microbiome of Deep Caries

Alexandru-Andrei

Perlea²,

Gheorghiu³

et al. 2019

ARS Medica Tomitana

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Deep caries progress is associated with tertiary dentin formation and additional reversible or irreversible dental pulp infl ammation. It seems that some particular signs of pain in irreversible pulpitis are associated to a particular caries microfl ora. Streptococcus species, Parvimonas micra and Dialister invisus are prevailing in cases of throbbing pain while Streptococcus mutans is incriminated in sensitivity to vertical percussion of tooth. Continuous pain is thought to be the clinical outcome of Lactobacillus implication. A better understanding of molecular signals and mechanisms induced by microbiome of deep caries that orchestrate the modulation of dental pulp complex response toward tertiary dentinogenesis or pulp infl ammation it is supposed to improve diagnosis and conservative therapies of vital pulp.

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Microbial Biofilms in Root Canal Systems

Paz¹

2022

Endodontic Advances and Evidence‐Based Clinical Guidelines

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Pyrosequencing Analysis of Cryogenically Ground Samples from Primary and Secondary/Persistent Endodontic Infections

Cited by 68 publications

References 36 publications

A Systematic Review of the Root Canal Microbiota Associated with Apical Periodontitis: Lessons from Next‐Generation Sequencing

A Systematic Review of the Root Canal Microbiota Associated with Apical Periodontitis: Lessons from Next‐Generation Sequencing

Molecular Mechanisms of Dentine-Pulp Complex Response Induced by Microbiome of Deep Caries

Microbial Biofilms in Root Canal Systems

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