A self-assembled multilayer based on a mixed MHDA-Biotinyl PE self-assembled monolayer followed by the addition of a biotin-avidin system was built up on a the gold electrode of a quartz crystal microbalance which was used to monitor the deposition.With the view to producing an odorant sensing device, an olfactory receptor (OR), ORI7, was immobilized on the self-assembled multilayer. The ORI7 OR originates from a large group of proteins belonging to the I subfamily of G protein coupled receptors that binds odorant ligands.All steps formation were followed with the QCM-D. Valuable information was obtained regarding the composition of each layer, providing evidence of the high dissipation effect of nanosomes adhesion. Also, based on the results, an explanation for multilayer formation and binding relations between components is proposed.