QseB/QseC: a two-component system globally regulating bacterial behaviors

Zhu, Yuxiang; Dou, Qin; Du, Liangcheng; Wang, Yan

doi:10.1016/j.tim.2023.02.001

Cited by 22 publications

(7 citation statements)

References 98 publications

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“…The periplasmic QseC domain is preserved among several species of Gram-negative bacteria such as enteropathogenic E. coli (serotype O26: H11 and O111ac: H9), Shigella spp., Salmonella spp., S. enterica Typhimurium, S. typhi , E. cloacae , Yersinia pestis , Y. enterocolitica , Pasteurella multocida and H. influenzae [ 135 ]. AI-3 acts similarly to eukaryotic hormones since QseC is a bacterial adrenergic receptor for the eukaryotic host hormones epinephrine and noradrenaline [ 46 , 136 ]. Another consequence of this structural similarity is that AI-3 is inhibited by adrenergic receptor antagonists [ 135 , 137 ].…”

Section: Genetic Modules and Their Homologues As Regulatory Network D...mentioning

confidence: 99%

“…In addition, epinephrine/norepinephrine can provide a QS signal to the quorum of gut microbiota and activate the QseC/QseB system [ 137 , 138 ]. Enterohemorrhagic E. coli O157:H7 (EHEC) use human hormones such as epinephrine and noradrenaline to activate virulence genes [ 136 , 139 ], which can be associated with irritable bowel syndrome induced by chronic stress and the stress hormone cascade [ 132 ]. In E. coli , mobility and virulence are regulated by QS using an Al-3 signalling molecule [ 46 , 134 , 136 ].…”

Section: Genetic Modules and Their Homologues As Regulatory Network D...mentioning

confidence: 99%

“…Enterohemorrhagic E. coli O157:H7 (EHEC) use human hormones such as epinephrine and noradrenaline to activate virulence genes [ 136 , 139 ], which can be associated with irritable bowel syndrome induced by chronic stress and the stress hormone cascade [ 132 ]. In E. coli , mobility and virulence are regulated by QS using an Al-3 signalling molecule [ 46 , 134 , 136 ]. In the presence of AI-3, the QseC domain undergoes autophosphorylation and then, by phosphorylating QseB, induces the transcription of the main flhDC regulon located in the locus of enterocyte effacement (LEE), which is responsible for cilia biosynthesis, cell mobility, and synthesis Shiga toxin [ 140 ].…”

Section: Genetic Modules and Their Homologues As Regulatory Network D...mentioning

confidence: 99%

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Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on Quorum Sensing (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination

Juszczuk-Kubiak

2024

IJMS

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One of the key mechanisms enabling bacterial cells to create biofilms and regulate crucial life functions in a global and highly synchronized way is a bacterial communication system called quorum sensing (QS). QS is a bacterial cell-to-cell communication process that depends on the bacterial population density and is mediated by small signalling molecules called autoinducers (AIs). In bacteria, QS controls the biofilm formation through the global regulation of gene expression involved in the extracellular polymeric matrix (EPS) synthesis, virulence factor production, stress tolerance and metabolic adaptation. Forming biofilm is one of the crucial mechanisms of bacterial antimicrobial resistance (AMR). A common feature of human pathogens is the ability to form biofilm, which poses a serious medical issue due to their high susceptibility to traditional antibiotics. Because QS is associated with virulence and biofilm formation, there is a belief that inhibition of QS activity called quorum quenching (QQ) may provide alternative therapeutic methods for treating microbial infections. This review summarises recent progress in biofilm research, focusing on the mechanisms by which biofilms, especially those formed by pathogenic bacteria, become resistant to antibiotic treatment. Subsequently, a potential alternative approach to QS inhibition highlighting innovative non-antibiotic strategies to control AMR and biofilm formation of pathogenic bacteria has been discussed.

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Section: Genetic Modules and Their Homologues As Regulatory Network D...mentioning

confidence: 99%

Section: Genetic Modules and Their Homologues As Regulatory Network D...mentioning

confidence: 99%

Section: Genetic Modules and Their Homologues As Regulatory Network D...mentioning

confidence: 99%

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Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on Quorum Sensing (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination

Juszczuk-Kubiak

2024

IJMS

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“…The two-component response regulator QseB, which promotes the expression of various virulence genes in S. Enteritidis (Weigel and Demuth, 2016), was upregulated in EL5 vs. WT and EL4-A2. The increase in expression of OmpR/EnvZ, UspC, and QseB, which are involved in stress adaptation and antibiotic resistance (Su et al, 2007;Viveiros et al, 2007;Deng et al, 2013;Zhu et al, 2023), possibly indirectly contributed to reduced antibiotic susceptibility in the ELs.…”

Section: Figurementioning

confidence: 99%

Transcriptomic analysis using RNA sequencing and phenotypic analysis of Salmonella enterica after acid exposure for different time durations using adaptive laboratory evolution

Ghoshal,

Bechtel,

Gibbons

et al. 2024

Front. Microbiol.

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IntroductionThis study is the final part of a two-part series that delves into the molecular mechanisms driving adaptive laboratory evolution (ALE) of Salmonella enterica in acid stress. The phenotypic and transcriptomic alterations in the acid-evolved lineages (EL) of Salmonella enterica serovar Enteritidis after 70 days of acid stress exposure were analyzed.Materials and methodsThe stability of phenotypic changes observed after 70 days in acetic acid was explored after stress removal using a newly developed evolutionary lineage EL5. Additionally, the impact of short-term acid stress on the previously adapted lineage EL4 was also examined.ResultsThe results indicate that the elevated antibiotic minimum inhibitory concentration (MIC) observed after exposure to acetic acid for 70 days was lost when acid stress was removed. This phenomenon was observed against human antibiotics such as meropenem, ciprofloxacin, gentamicin, and streptomycin. The MIC of meropenem in EL4 on day 70 was 0.094 mM, which dropped to 0.032 mM when removed from acetic acid stress after day 70. However, after stress reintroduction, the MIC swiftly elevated, and within 4 days, it returned to 0.094 mM. After 20 more days of adaptation in acetic acid, the meropenem MIC increased to 0.125 mM. The other human antibiotics that were tested exhibited a similar trend. The MIC of acetic acid in EL4 on day 70 was observed to be 35 mM, which remained constant even after the removal of acetic acid stress. Readaptation of EL4 in acetic acid for 20 more days caused the acetic acid MIC to increase to 37 mM. Bacterial whole genome sequencing of EL5 revealed base substitutions in several genes involved in pathogenesis, such as the phoQ and wzc genes. Transcriptomic analysis of EL5 revealed upregulation of virulence, drug resistance, toxin-antitoxin, and iron metabolism genes. Unstable Salmonella small colony variants (SSCV) of S. Enteritidis were also observed in EL5 as compared to the wild-type unevolved S. Enteritidis.DiscussionThis study presents a comprehensive understanding of the evolution of the phenotypic, genomic, and transcriptomic changes in S. Enteritidis due to prolonged acid exposure through ALE.

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“…The PhoPQ regulatory network has adapted in several ways to accommodate the variable roles it plays in different organisms, including changes to its signal sensing, signal transduction, and DNA-binding properties, and the acquisition of different connector proteins that integrate PhoPQ signalling with other TCS [15,16,20,[22][23][24]. This example is not unique, and different TCSs face variable evolutionary pressures depending upon their unique biology and the unique challenges faced by the organisms that encode them [25][26][27][28]. With few exceptions, very little is known about how the functional properties of TCSs differ in different organisms.…”

Section: Introductionmentioning

confidence: 99%

Conserved patterns of sequence diversification provide insight into the evolution of two-component systems in Enterobacteriaceae

Barretto,

Van,

Fowler

2024

Microbial Genomics

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Two-component regulatory systems (TCSs) are a major mechanism used by bacteria to sense and respond to their environments. Many of the same TCSs are used by biologically diverse organisms with different regulatory needs, suggesting that the functions of TCS must evolve. To explore this topic, we analysed the amino acid sequence divergence patterns of a large set of broadly conserved TCS across different branches of Enterobacteriaceae, a family of Gram-negative bacteria that includes biomedically important genera such as Salmonella, Escherichia, Klebsiella and others. Our analysis revealed trends in how TCS sequences change across different proteins or functional domains of the TCS, and across different lineages. Based on these trends, we identified individual TCS that exhibit atypical evolutionary patterns. We observed that the relative extent to which the sequence of a given TCS varies across different lineages is generally well conserved, unveiling a hierarchy of TCS sequence conservation with EnvZ/OmpR as the most conserved TCS. We provide evidence that, for the most divergent of the TCS analysed, PmrA/PmrB, different alleles were horizontally acquired by different branches of this family, and that different PmrA/PmrB sequence variants have highly divergent signal-sensing domains. Collectively, this study sheds light on how TCS evolve, and serves as a compendium for how the sequences of the TCS in this family have diverged over the course of evolution.

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QseB/QseC: a two-component system globally regulating bacterial behaviors

Cited by 22 publications

References 98 publications

Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on Quorum Sensing (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination

Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on Quorum Sensing (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination

Transcriptomic analysis using RNA sequencing and phenotypic analysis of Salmonella enterica after acid exposure for different time durations using adaptive laboratory evolution

Conserved patterns of sequence diversification provide insight into the evolution of two-component systems in Enterobacteriaceae

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