QTL Analysis for Resistance to Phytophthora capsici in Pepper Using a High Density SSR-based Map

Abstract

A segregating doubled haploid (DH) population (n = 96) was developed by anther culture of an F 1 plant crossed between susceptible ('Manganji') and resistant ('Criollo de Morelos 334') lines of pepper (Capsicum annuum L.) to conduct a genetic analysis of resistance to Phytophthora rot caused by Phytophthora capsici. In order to perform a quantitative trait locus (QTL) analysis, we constructed a high density simple sequence repeat (SSR)-based map with a total length of 878 cM. Sixteen linkage groups (LGs) and 118 SSR markers were located using the 626 SSR markers that we previously developed. Resistance was evaluated in two root inoculation tests. Interval mapping for the resistance to P. capsici detected a common major QTL in the duplicate tests and a minor QTL specific to the first test. The major QTL was located on LG15 and flanked with an SSR marker, CAMS420. In addition, seven SSR markers were located within 21 cM intervals from the peak of this QTL. In contrast, the QTL on LG3 was detected with small effects in the first test, the nearest marker was a dominant amplified fragment length polymorphism (AFLP) marker, and the QTL was surrounded by eight SSR markers within a distance of 10 cM. Since some of the linkage markers for agriculturally valuable traits cannot detect polymorphism within breeding populations in C. annuum, the present linkage markers may widen the choice in marker-assisted selection in breeding programs for Phytophthora rot resistant pepper cultivars.

Mapping for Axillary Shooting, Flowering Date, Primary Axis Length, and Number of Leaves in Pepper (Capsicum annuum)

“…Previously published SSR markers were used for the present mapping (Huang et al, 2001;Lee et al, 2004;Minamiyama et al, 2006aMinamiyama et al, , 2007Nagy et al, 2007;Yi et al, 2006). In addition, AFLP and some of the newly developed SSR markers were used.…”

“…Hence, the inheritance of these traits has been extensively studied and the quantitative trait loci (QTLs) that contribute to disease resistance and fruit traits have been reported (Ben Chaim et al, 2001a, 2001bLefebvre et al, 2003;Minamiyama et al, 2005Minamiyama et al, , 2007Popovsky and Paran, 2000;Thabuis et al, 2003;Voorrips et al, 2004). In addition to the above-mentioned traits, axillary shooting, flowering date, primary axis length, and number of leaves are also important for pepper production.…”

Identificación de fuentes de resistencia a pudriciones de la raíz en germoplasma de chile serrano (Capsicum annuum L.)

“…Despite these genetic material did not amplify the SCAR marker OpD04.717, can be used to search for other markers associated with genes that confer resistance to the Oomycete like: CaRGA2, CABPR1, CABGLU, CAPO1, CASC1 (Silvar et al, 2008;Zhang et al, 2013), or QTLs Phyto.4.1, Phyto.5.1, Phyto.6.1, Phyto.11.1 and Phyto12.1, located on chromosomes 4, 5, 11 and 12 respectively (Thabuis et al 2003). Because the resistance to this pathogen is polygenic (Pochard and Daubèze, 1980;Ogundiwin et al, 2005;Minamiyama et al, 2007), it is difficult to explain the resistance to the disease just through marker OpD04.717.…”

“…A pesar que estos materiales genéticos no amplificaron el marcador SCAR OpD04.717, pueden ser utilizados para la búsqueda de otros marcadores asociados a genes que confieren resistencia al Oomycete como: CaRGA2, CABPR1, CABGLU, CAPO1, CASC1 (Silvar et al, 2008;Zhang et al, 2013), o a los QTLs Phyto.4.1, Phyto.5.1, Phyto.6.1, Phyto.11.1 y Phyto12.1, localizadas en los cromosomas 4, 5, 11 y 12, respectivamente (Thabuis et al, 2003). Debido a que la resistencia a este fitopatógeno es poligénica (Pochard y Daubèze, 1980;Minamiyama et al, 2007), es difícil explicar la resistencia a la enfermedad mediante únicamente el marcador OpD04.717.…”

The disease reactions of heirloom bell pepper “California Wonder” to <i>Phytophthora capsici</i>

“…Twenty-two previously published SSR primer pairs [11][12][13] were tested as described previously [14] and 9 were found to be polymorphic within or between California Wonder seed lots ( Table 2).…”