QTL detection and MAS selection efficiency for lipid content in brown rice (Oryza sativa L.)

Yang, Qin; Kim, Suk‐Man; Zhao, Xinhua; Lee, Hyun-Suk; Jia, Bin; Kim, Kyung Min; Eun, Moo-Young; Sohn, Jae-Keun

doi:10.1007/s13258-010-0026-5

Cited by 14 publications

(13 citation statements)

References 11 publications

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“…All measurements were performed with three replicates. FA extraction was performed as described by Yang et al [2]. Quantification of FA A c c e p t e d M a n u s c r i p t 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 11 content was performed using GC-MS by assaying fatty acid methyl esters (FAMEs) as described by Ying et al [5].…”

Section: Analysis Of the Starch Protein And Total Lipid Contents Of mentioning

confidence: 99%

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A lipid transfer protein, OsLTPL36, is essential for seed development and seed quality in rice

Wang

Zhou

et al. 2015

Plant Science

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Section: Analysis Of the Starch Protein And Total Lipid Contents Of mentioning

confidence: 99%

“…Among all the nutritional components in rice endosperm, crude fat content (CFC) is one major substance. CFC or lipids are mostly deposited in bran although their amounts are fewer than that of starch and protein in bran [2]. Fatty acid (FA) in brown rice is an important ingredient of functional foods and industrial products [3].…”

Section: Introductionmentioning

confidence: 99%

A lipid transfer protein, OsLTPL36, is essential for seed development and seed quality in rice

Wang

Zhou

et al. 2015

Plant Science

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“…Rice lipid is mainly concentrated in the embryo, bran, and aleurone layer. Brown rice is rich in oleic acid, linoleic acid, and various trace components such as γ-oryzanol, sterols, tocopherol, and tocotorienol [2,3]. In general, lipid content amounts to 1.6% -3.02% at 14% moisture in brown rice [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…In general, lipid content amounts to 1.6% -3.02% at 14% moisture in brown rice [4][5][6]. The lipids found in rice, especially unsaturated fatty acids, greatly influence grain appearance and quality [3,[6][7][8][9][10][11]. Changes in the crude fat content of rice are associated with aging and deterioration of rice.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Characteristics and QTL Mapping Associated with the Lipid Content of High-Lipid Rice

Kim¹,

Sohn²,

Kim³

2013

AJPS

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This study was conducted to examine the physicochemical characteristics and perform QTL mapping of genetic factors associated with the lipid content of rice. A rice strain with a high lipid content, "P31-2-2-2-B-B", was developed from mutants of "Dongjin" created by T-DNA insertion. The lipid content of "P31-2-2-2-B-B" brown rice was 4.42% whereas that of the donor cultivar "Dongjin" was 2.56%. The total fatty acid content of the high-lipid mutant brown rice was 7.82% and that of "Dongjin" was 3.43%. The unsaturated fatty acid composition of the mutant brown rice was 2.73% oleic acid, 2.74% linoleic acid, and 0.34% linolenic acid. In contrast, the fatty acid composition of the donor cultivar "Dongjin" was 1.30% oleic acid and 0.99% linoleic acid. The percentage of unsaturated fatty acid to total fatty acid in the high-lipid mutant was higher (74.3%) than that of "Dongjin" (66.8%). Continuous frequency distribution and transgressive segregation of the lipid content were observed in the F 3 family (seeds) derived from a cross between the high-lipid mutant "P31-2-2-2-B-B" and a tongil-type cultivar "Samgang". This result implied that the lipid content was a quantitative trait controlled by a polygene. Additionally, the broad sense heritability of lipid content was estimated to be 89.6% based on analysis of the F 3 seeds. A significant QTL, qRLC5, was identified on chromosome 5 with a LOD score of 2.37, and was flanked by 5007 and 5014. Results of the present study should be useful for improving rice nutritional quality through marker-assisted selection.

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“…In addition, a major QTL for protein content in rice, qPC1 , has been cloned and functionally characterized [ 46 ]. A major QTL responsible for crude fat content (FC) in brown rice, qCFC5 , is located on chromosome five [ 47 ]. QTLs controlling FC are governed by time-dependent gene expression; Wang et al [ 48 ] revealed different expression patterns of FC-related QTLs at the grain filling stage.…”

Section: Genetics and Genomics Of Rice Grain Qualitymentioning

confidence: 99%

Applications of the CRISPR/Cas9 System for Rice Grain Quality Improvement: Perspectives and Opportunities

Fiaz

Ahmad

Noor

et al. 2019

IJMS

110

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Grain quality improvement is a key target for rice breeders, along with yield. It is a multigenic trait that is simultaneously influenced by many factors. Over the past few decades, breeding for semi-dwarf cultivars and hybrids has significantly contributed to the attainment of high yield demands but reduced grain quality, which thus needs the attention of researchers. The availability of rice genome sequences has facilitated gene discovery, targeted mutagenesis, and revealed functional aspects of rice grain quality attributes. Some success has been achieved through the application of molecular markers to understand the genetic mechanisms for better rice grain quality; however, researchers have opted for novel strategies. Genomic alteration employing genome editing technologies (GETs) like clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) for reverse genetics has opened new avenues of research in the life sciences, including for rice grain quality improvement. Currently, CRISPR/Cas9 technology is widely used by researchers for genome editing to achieve the desired biological objectives, because of its simple targeting. Over the past few years many genes that are related to various aspects of rice grain quality have been successfully edited via CRISPR/Cas9 technology. Interestingly, studies on functional genomics at larger scales have become possible because of the availability of GETs. In this review, we discuss the progress made in rice by employing the CRISPR/Cas9 editing system and its eminent applications. We also elaborate possible future avenues of research with this system, and our understanding regarding the biological mechanism of rice grain quality improvement.

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QTL detection and MAS selection efficiency for lipid content in brown rice (Oryza sativa L.)

Cited by 14 publications

References 11 publications

A lipid transfer protein, OsLTPL36, is essential for seed development and seed quality in rice

A lipid transfer protein, OsLTPL36, is essential for seed development and seed quality in rice

Physicochemical Characteristics and QTL Mapping Associated with the Lipid Content of High-Lipid Rice

Applications of the CRISPR/Cas9 System for Rice Grain Quality Improvement: Perspectives and Opportunities

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